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EMAGE:22011

Sema3d sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D ( MGI:1860118)
TS22 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images EMAGE genex expression entry
Expression pattern clarity: no stars
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:Sema3d
Entity Detected:Sema3d, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D ( MGI:1860118)
Sequence:sense strand is shown

>Sema3d
ATTGAGTAGATGACCAAGGTGGGGAGTGGGGCTATATATTTGTTGAATGGATGGATAATGGCAAAATTTT
GATGACGGGAAGGGTTAAATTAACTCTTCGACATCCTCTCGTTACATAAACTTTCAAGCAGTGTTGTTTT
CAGCAGTTTCACAAGGAAGTCTTCAACATCTAAAGAAAACACTCACACCGTTAGCATAAGTACCATTTGT
ATTTTGCTAGCCTGTGTCAAATTCAACTTTGCCTTTGAACAGTGTTTTCCTGCCAGTCTCTCCAGAAATC
AACTTTCCTTCCTGTAGCACAAAACCCTTAGCCTTCCTCTGTTTTGCCTTTTCACGCTCTTTATAGTGTG
AAATGAACAATTAGTCACTTCCTCACAAAGAATGCAGCTTTTTAGAAAACCAACAGACCTTGTTTGTTTC
TCATGCATTCTACATGTTTTGAAAGATTCTGTGAGAAGCCTGTGTGATTAAAAAGCAGTTTTACAGAGTC
AAGCAATCTATCTCTTCAAAGTAGCATCAGTATTTTACATTCATTTAATTTGCATTGGGTACCTATCTCA
AGGGCATAACATTATCTGCAAGGGAC
nt 4011 - nt 4597 of NM_028882.4
Notes:Forward Primer sequence:GTCCCTTGCAGATAATGTTATGCCC. Reverse Primer sequence:GTCCCTTGCAGATAATGTTATGCCC
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6-Tyr
<
c-Brd
>
Age:14.5 dpc
Theiler Stage:TS22
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:parafin
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dongrong Chen, Diane Gleeson, Elizabeth Huckle, Anja-Kolb Kokocinksi, David Tannahill
Principal investigator:David Tannahill, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK CB10 1SA
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
Links:EmbryoExpress same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMBRYOEXPRESS