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EMAGE:2256

Klf10 Kruppel-like factor 10 ( MGI:1101353)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:2256 EMAGE:2256
1467d1.jpg This embryo was treated with ProteinaseK for either 3min or 30min. 1467d2.jpg This embryo was treated with ProteinaseK for either 3min or 30min.

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:2256Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
2256_wholemount_strong_3D_1.wlz
2256_wholemount_moderate_3D_1.wlz
2256_wholemount_weak_3D_1.wlz
2256_wholemount_possible_3D_1.wlz
2256_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:2256_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
central nervous system
possible possible
Authors stated that in some instances they could not determine expression levels with certainty.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3506931
Entity Detected:Klf10, Kruppel-like factor 10 ( MGI:1101353)
Sequence:sense strand is shown

>MGI:3506931
CCCAAGGGTCAAATCTGACTGCATCAGCGCCATCTACTGGCCACTTCAAATCTTTCTCCGATGCTGCCAA
GCCTCCAGGCGCCACTCCTTTCAAAGAGGAGGAAAAGAATCCTTTAGCTGCCCCTCCTCTTCCTAAGGCT
CAAGCCACCAGTGTCATCCGTCACACAGCTGATGCCCAACTGTGCAACCACCAGTCCTGCCCCGTGAAAG
CAGCTAGCATCCTCAACTATCAGGACAATTCTTTCCGGAGAAGAACCCACGGAAATGTTGAGGCTACTCG
AAAGAACATACCCTGTGCTGCAGTGTCACCAAACAGATCCAAGCCTGAGCCCAGCACAGTGTCCGATGGT
GATGAGAAGGCGGGCGCTGCACTATATGACTTTGCTGTGCCTTCCTCAGAGACAGTAATTTGTAGGTCTC
AGCCAGCTCCTTCGTCCCCAGTGCAGAAGTCAGTACTGGTGTCTTCACCTACAGTATCCACTGGGGGAGT
GCCACCCCTGCCTGTCATCTGCCAGATGGTTCCCCTTCCTGCCAACAACTCTCTTGTTAGCACAGTTGTC
CCCAGCACTCCTCCTAGCCAGCCACCAGCTGTCTGCTCACCTGTGTTGTTCATGGGCACTCAGGTGCCTG
AGGGCACCGTCGTGTTTGTGGTACCCCAGCCCGTTGTGCAGAGCCCAAGGCCTCCAGTGGTGAGCCCCAG
TGGCACCAGACTGTCTCCCATTGCCCCTGCTCCTGGATTCTCTC
nt 421 - nt 1164 of NM_013692.1
Notes:The template DNA sequence was PCR amplified from E13.5 or P0 mouse brain, embryonic kidney or testis cDNA using: 5' primer: CCCAAGGGTCAAATCTGACTGC 3'primer: GAGAGAATCCAGGAGCAGGGG
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Yu J., Tenzen T., Gray P.A., Fu H., Luo P., Zhao Q., Ferrari A., Yuk D.I., Tsung E.F., Cai Z., Alberta J.A., Cheng L.P., Liu Y., Stenman J.M., Valerius M.T., Billings N., Kim H.A., Greenberg M.E., Rowitch D.H., Stiles C.D., Ma Q., McMahon A.P. Indexed by GXD, Spatially mapped by EMAGE.
Principal investigator:Andrew P. McMahon, Molecular and Cellular Biology, Harvard, Department of Molecular and Cellular Biology Harvard University 16 Divinity Ave Room 1059, Cambridge, U.S.A MA 02138
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1126/science.1104935] [ PMID:15618518] Gray PA, 2004 Mouse brain organization revealed through direct genome-scale TF expression analysis. Science (306):2255-7
Acknowledgments:This work was supported by the Charles Dana Foundation, the National Institute of Neurological Disorders and Stroke (QM, CDS, DHR & APM), the National Institute of Dental and Craniofacial Research (QM), the National Institute of Diabetes and Digestive and Kidney Diseases (APM), a Ford Foundation Postdoctoral Fellowship for Minorities (PAG), a Parker B. Francis Fellowship in Pulmonary Medicine (PAG) and the Pew Trust (QM).
Links:MGI:3509631 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE