Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:226

Fgf4 fibroblast growth factor 4 ( MGI:95518)
TS10
in situ hybridisation

Data Images
EMAGE:226 EMAGE:226
Fig 3E of Niswander & Martin, 1992 [PMID:1618140] . Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Fig 3D of Niswander & Martin, 1992 [PMID:1618140] . Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
This is a mid-streak embryo. Corresponding bright- and dark-field images shown below. Abbreviations used: ec, embryonic ectoderm; em, embryonic mesoderm; pe, parietal endoderm; ps, primitive streak; ve, visceral endoderm; *, promamniotic cavity. Arrowheads indicate approximate lateral limits of the primitive streak.
Expression Pattern Description
Spatial Annotation:
EMAGE:226EMAGE:226Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
226_voxel_strong_3D_1.wlz
226_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:226_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
embryo
not detected not detected
Fgf4 expression was never observed in the head process.
embryo ectoderm
detected detected
regionalFgf-4 RNA is limited to cells adjacent to positive regions of the primitive streak.
definitive endoderm
not detected not detected
extraembryonic component
not detected not detected
embryo mesoderm
detected detected
regionalFgf-4 RNA is not observed as cells move laterally around the embryo, except in those mesodermal cells emerging from the distal (rostral) regions of the primitive streak where expression continues within 4-6 cell diameters of the streak (data not shown).
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:17265
Entity Detected:Fgf4, fibroblast growth factor 4 ( MGI:95518)
Notes:Two Fgf4 probes were used by Niswander et al, 1992 [PMID:1618140] , each giving "identical results": the"full length coding sequence (see Hebert et al, 1990 [PMID:2318343] " and "the 3' untranslated region from nt 3630-3819 (see Brookes et al, 1989 [PMID:2740210] ". Unable to ascertain which was used here.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:Swiss Webster
Theiler Stage:TS10
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Niswander & Martin, 1992 [PMID:1618140] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1618140] Niswander L, Martin GR 1992 Fgf-4 expression during gastrulation, myogenesis, limb and tooth development in the mouse. Development (114):755-68
 [ doi:10.1016/0012-1606(90)90211-Z] [ PMID:2318343] H�bert JM, Basilico C, Goldfarb M, Haub O, Martin GR 1990 Isolation of cDNAs encoding four mouse FGF family members and characterization of their expression patterns during embryogenesis. Dev Biol (138):454-63
 [ doi:10.1093/nar/17.11.4037] [ PMID:2740210] Brookes S, Smith R, Thurlow J, Dickson C, Peters G 1989 The mouse homologue of hst/k-FGF: sequence, genome organization and location relative to int-2. Nucleic Acids Res (17):4037-45
Links:MGI:1331200 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI