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EMAGE:229

Fgf4 fibroblast growth factor 4 ( MGI:95518)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:229 EMAGE:229 EMAGE:229
Fig 5F of Niswander & Martin, 1992 [PMID:1618140] . Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Fig 5A of Niswander & Martin, 1992 [PMID:1618140] . Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Fig 5E of Niswander & Martin, 1992 [PMID:1618140] . Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
Corresponding bright and darkfield images shown below. Section plane is "oblique frontal" and is depicted in Panel A below. Abbreviations used: d, dermotome; m, myotome; scl, sclerotome.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
regionalExpression continues in myotomal cells, but Fgf-4 RNA becomes limited to a sub-population of the myotome, as illustrated by the observation that only a fraction of the cells that are positive for Actc-1 RNA are also Fgf-4 positive.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:17265
Entity Detected:Fgf4, fibroblast growth factor 4 ( MGI:95518)
Notes:Two Fgf4 probes were used by Niswander et al, 1992 [PMID:1618140] , each giving "identical results": the"full length coding sequence (see Hebert et al, 1990 [PMID:2318343] " and "the 3' untranslated region from nt 3630-3819 (see Brookes et al, 1989 [PMID:2740210] ". Unable to ascertain which was used here.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:Swiss Webster
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Niswander & Martin, 1992 [PMID:1618140] . Indexed by GXD.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1618140] Niswander L, Martin GR 1992 Fgf-4 expression during gastrulation, myogenesis, limb and tooth development in the mouse. Development (114):755-68
 [ doi:10.1016/0012-1606(90)90211-Z] [ PMID:2318343] H�bert JM, Basilico C, Goldfarb M, Haub O, Martin GR 1990 Isolation of cDNAs encoding four mouse FGF family members and characterization of their expression patterns during embryogenesis. Dev Biol (138):454-63
 [ doi:10.1093/nar/17.11.4037] [ PMID:2740210] Brookes S, Smith R, Thurlow J, Dickson C, Peters G 1989 The mouse homologue of hst/k-FGF: sequence, genome organization and location relative to int-2. Nucleic Acids Res (17):4037-45
Links:MGI:1331200 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE