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EMAGE:23720

Igfbp5 insulin-like growth factor binding protein 5 ( MGI:96440)
TS13 (8.5 dpc)
in situ hybridisation

Data Images
EMAGE:23720 EMAGE:23720 EMAGE:23720
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Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:23720Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
23720_wholemount__moderate.wlz
23720_wholemount__weak.wlz
23720_wholemount__notDetected.wlz
23720_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:23720_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Expression is widespread, but is stronger in the notochord, somites, and endoderm than elsewhere in the embryo.
notochord
detected detected
Expression is stronger in the notochord, somites, and endoderm than elsewhere in the embryo.
somite
detected detected
Expression is stronger in the notochord, somites, and endoderm than elsewhere in the embryo.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3069565
Entity Detected:Igfbp5, insulin-like growth factor binding protein 5 ( MGI:96440)
Notes:The Igfbp5 probe template used in this study by Tamplin et al, was PCR amplified from NIA clone H3126F05 using the vector (pSPORT1) specific primers: pSPORT1 FWD GCTATTACGCCAGCTGGCGAAAGGGGGATGTG and pSPORT1 REV CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. SP6 polymerase was used to generate antisense probe.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:8.5 dpc
Theiler Stage:TS13
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Owen J. Tamplin, Brian J. Cox, Janet Rossant.
Principal investigator:Janet Rossant, The Hospital for Sick Children Research Institute, TMDT at MaRS Room 13-305 101 College Street, Toronto, ON, Canada M5G1L7
Submitted by:Owen Tamplin, HHMI/Children's Hospital Boston, Zon Lab, Division of Hematology/Oncology 300 Longwood Avenue, Karp 8, Boston, MA, USA 02115
Experiment type:screen
References:[ doi:10.1016/j.ydbio.2011.10.002] [ PMID:22008794] Tamplin OJ, Cox BJ, Rossant J 2011 Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord. Dev Biol (360)
Links:MGI:5291783 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE