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EMAGE:23738

Piwil4 piwi-like homolog 4 (Drosophila) ( MGI:3041167)
TS12 (8.0 dpc)
in situ hybridisation

Data Images
EMAGE:23738 EMAGE:23738
Lateral view Posterior Ventral Detail View

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:23738Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
23738_wholemount__moderate.wlz
23738_wholemount__weak.wlz
23738_wholemount__notDetected.wlz
23738_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:23738_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
unsegmented mesenchyme
detected detected
Expression is stronger in the presomitic mesoderm than elsewhere in the embryo.
embryo
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3550365
Entity Detected:Piwil4, piwi-like homolog 4 (Drosophila) ( MGI:3041167)
Notes:The Piwil4 probe template used in this study by Tamplin et al, was PCR amplified from RIKEN clone E030032F21 using the vector (pBS KS+) specific primers: pBS FWD GTTTTCCCAGTCACGACGTT and pBS REV TGTGGAATTGTGAGCGGATA. T3 polymerase was used to generate antisense probe.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:8.0 dpc
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Owen J. Tamplin, Brian J. Cox, Janet Rossant.
Principal investigator:Janet Rossant, The Hospital for Sick Children Research Institute, TMDT at MaRS Room 13-305 101 College Street, Toronto, ON, Canada M5G1L7
Submitted by:Owen Tamplin, HHMI/Children's Hospital Boston, Zon Lab, Division of Hematology/Oncology 300 Longwood Avenue, Karp 8, Boston, MA, USA 02115
Experiment type:screen
References:[ doi:10.1016/j.ydbio.2011.10.002] [ PMID:22008794] Tamplin OJ, Cox BJ, Rossant J 2011 Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord. Dev Biol (360)
Links:MGI:5291786 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE