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EMAGE:238

Furin furin (paired basic amino acid cleaving enzyme) ( MGI:97513)
TS11 (7.5 dpc)
in situ hybridisation

Data Images
EMAGE:238 EMAGE:238 EMAGE:238
Figure 1A of Roebroek et al., 1998 [PMID:9811571] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Figure 1B of Roebroek et al., 1998 [PMID:9811571] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Figure 1C of Roebroek et al., 1998 [PMID:9811571] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
The embryo in Panel A was sectioned transversely. The relative positions of section B (upper line in A) and section C (lower line in A) are indicated. Abbreviations used: ave, anterior visceral endoderm; cf, chorionic fold; e and ee, embryonic and extraembryonic region; em, extraembryonic mesoderm; ep, epiblast; m, mesoderm; n, node.
Expression Pattern Description
Spatial Annotation:
EMAGE:238Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
238_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:238_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
extraembryonic component
detected detected
regionalExpression is localized to the mesoderm and primitive endoderm
embryo
strong strong
regionalExpression is limited to the proximal region. Authors describe expression in ectodermal cells in antero-proximal region of the epiblast.
node
detected detected
homogeneousA diffuse signal is detected at the node.
heart
strong strong
regionalExpression is seen in the precardiac mesoderm
primitive endoderm
strong strong
No expression is seen in the most proximal primitive endoderm contacting the extraembryonic ectoderm
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1309863
Entity Detected:Furin, furin (paired basic amino acid cleaving enzyme) ( MGI:97513)
Sequence:sense strand is shown

>MGI:1309863
GGATCCGGCTGGCGAATGGGTCCTAGAGATTGAAAATACCAGTGAAGCCAACAACTATGGGACGCTGACC
AAGTTCACTCTCGTTCTGTATGGCACAGCCCCCGAGGGGCTCTCTACACCTCCAGAAAGCAGCGGCTGCA
AGACCCTCACATCCAGCCAGGCCTGCGTGGTGTGTGAGGAAGGTTACTCTCTGCACCAGAAAAGCTGTGT
CCAGCACTGCCCACCAGGCTTCATCCCCCAAGTCCTTGATACACACTACAGCACTGAGAATGATGTGGAG
ATCATCCGTGCCAGCGTCTGTACCCCCTGCCACGCCTCATGTGCCACTTGCCAGGGCCCGGCCCCCACAG
ACTGCCTCAGCTGCCCCAGCCATGCCTCCCTGGACCCAGTGGAGCAGACCTGCTCCCGGCAAAGCCAGAG
CAGCCGGGAGTCTCGGCCTCAGCAGCAGCCTCCTGCACTGCGCCCTGAGGTGGAGATGGAGCCCCGGCTG
CAGGCTGGGTTGGCCTCTCACCTGCCCGAGGTCCTGGCCGGCCTCAGCTGCCTTATCATCGTGCTCATCT
TTGGCATCGTCTTCCTGTTCCTGCATCGGTGTTCGGGCTTCAGCTTCCGGGGAGTGAAAGTGTACACCAT
GGACCGCGGCCTCATCTCCTACAAGGGGCTGCCCCCTGAAGCCTGGCAGGAGGAGTGCCCATCGGACTCA
GAGGAGGACGAGGGCCGGGGCGAGAGGACCGCCTTCATCAAAGACCAGAGCGCCCTTTGAC
nt 2014 - nt 2774 of L26489.1
Notes:The probe used in this study by Roebroek et al., 1998 [PMID:9811571] is described as "nucleotides 2014-2774, corresponding to the 3' end of the Furin coding sequence (gb:L26489)".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:7.5 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Roebroek et al., 1998 [PMID:9811571] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9811571] Roebroek AJ, Umans L, Pauli IG, Robertson EJ, van Leuven F, Van de Ven WJ, Constam DB 1998 Failure of ventral closure and axial rotation in embryos lacking the proprotein convertase Furin. Development (125):4863-76
Links:MGI:1329808 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI