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EMAGE:23801

Tmem176a transmembrane protein 176A ( MGI:1913308)
TS11 (7.5 dpc)
in situ hybridisation

Data Images
EMAGE:23801
Lateral view

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:23801Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
23801_wholemount__moderate.wlz
23801_wholemount__notDetected.wlz
23801_wholemount__strong.wlz
23801_wholemount__weak.wlz
(what is wlz format?)
Download all expression domains: EMAGE:23801_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
node
detected detected
embryo endoderm
detected detected
regionalExpression is present in the posterior endoderm.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3067117
Entity Detected:Tmem176a, transmembrane protein 176A ( MGI:1913308)
Notes:The Tmem176a probe template used in this study by Tamplin et al, was PCR amplified from NIA clone H3095F05 using the vector (pSPORT1) specific primers: pSPORT1 FWD GCTATTACGCCAGCTGGCGAAAGGGGGATGTG and pSPORT1 REV CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. SP6 polymerase was used to generate antisense probe.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:7.5 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Owen J. Tamplin, Brian J. Cox, Janet Rossant.
Principal investigator:Janet Rossant, The Hospital for Sick Children Research Institute, TMDT at MaRS Room 13-305 101 College Street, Toronto, ON, Canada M5G1L7
Submitted by:Owen Tamplin, HHMI/Children's Hospital Boston, Zon Lab, Division of Hematology/Oncology 300 Longwood Avenue, Karp 8, Boston, MA, USA 02115
Experiment type:screen
References:[ doi:10.1016/j.ydbio.2011.10.002] [ PMID:22008794] Tamplin OJ, Cox BJ, Rossant J 2011 Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord. Dev Biol (360)
Links:MGI:5291816 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE