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EMAGE:23830

Aim1 absent in melanoma 1 ( MGI:109544)
TS11 (7.5 dpc)
in situ hybridisation

Data Images
EMAGE:23830
Anterior View

Expression pattern clarity: no stars
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo endoderm
detected detected
Expression is stronger in the endoderm than elsewhere in the embryo.
embryo
detected detected
Expression was described as Widespread.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1374506
Entity Detected:Aim1, absent in melanoma 1 ( MGI:109544)
Notes:The Aim1 probe template used in this study by Tamplin et al, was PCR amplified from MGC clone 30437 using the vector (pCMV-SPORT6) specific primers: pCMV-SPORT6 FWD ACAAAGATCCCAAGCTAGCAG and pCMV-SPORT6 REV TTGACCTCCATAGAAGACACC. T7 polymerase was used to generate antisense probe.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:7.5 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Owen J. Tamplin, Brian J. Cox, Janet Rossant.
Principal investigator:Janet Rossant, The Hospital for Sick Children Research Institute, TMDT at MaRS Room 13-305 101 College Street, Toronto, ON, Canada M5G1L7
Submitted by:Owen Tamplin, HHMI/Children's Hospital Boston, Zon Lab, Division of Hematology/Oncology 300 Longwood Avenue, Karp 8, Boston, MA, USA 02115
Experiment type:screen
References:[ doi:10.1016/j.ydbio.2011.10.002] [ PMID:22008794] Tamplin OJ, Cox BJ, Rossant J 2011 Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord. Dev Biol (360)
Links:MGI:5291774 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE