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EMAGE:23864

Mnx1 motor neuron and pancreas homeobox 1 ( MGI:109160)
TS12 (8.0 dpc)
in situ hybridisation

Data Images
EMAGE:23864
Posterior Ventral Detail View

Expression pattern clarity: no stars
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
regionalExpression is present in the tail bud.
embryo endoderm
detected detected
notochord
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3829392
Entity Detected:Mnx1, motor neuron and pancreas homeobox 1 ( MGI:109160)
Notes:The Mnx1 probe template used in this study by Tamplin et al, was PCR amplified from clone UI-M-BH2.1-ape-d-05-0-UI.r1 using the vector (pT7T3D-PacI) specific primers: pT7T3D FWD GTTTTCCCAGTCACGACGTT and pT7T3D REV TGTGGAATTGTGAGCGGATA. T3 polymerase was used to generate antisense probe.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:8.0 dpc
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Owen J. Tamplin, Brian J. Cox, Janet Rossant.
Principal investigator:Janet Rossant, The Hospital for Sick Children Research Institute, TMDT at MaRS Room 13-305 101 College Street, Toronto, ON, Canada M5G1L7
Submitted by:Owen Tamplin, HHMI/Children's Hospital Boston, Zon Lab, Division of Hematology/Oncology 300 Longwood Avenue, Karp 8, Boston, MA, USA 02115
Experiment type:screen
References:[ doi:10.1016/j.ydbio.2011.10.002] [ PMID:22008794] Tamplin OJ, Cox BJ, Rossant J 2011 Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord. Dev Biol (360)
Links:MGI:5291677 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE