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EMAGE:239

Crabp1 cellular retinoic acid binding protein I ( MGI:88490)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:239
Figure 4B of Dupe et al., 1999 [PMID:10529422] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Abbreviations used: B1, B2 and B3, pharyngeal (branchial) arches one to three; B4/6, presumptive 4th and 6th pharyngeal arches; M, mesencephalon; PH, lumen of the pharynx. See EMAGE:240 for the whole mount view of this embryo.
Expression Pattern Description
Spatial Annotation:
EMAGE:239EMAGE:239Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
239_voxel_strong_3D_1.wlz
239_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:239_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
detected detected
single cellCRABPI is expressed in two broad subectodermal sheets of neural crest cells arising from rhombomeres 4, 6 & 7; they colonize the periphery of the 2nd, 3rd & 4th arches, respectively, enveloping negative mesenchymal cells derived from paraxial mesoderm.
2nd branchial arch
detected detected
single cellCRABPI is expressed in two broad subectodermal sheets of neural crest cells arising from rhombomeres 4, 6 & 7; they colonize the periphery of the 2nd, 3rd & 4th arches, respectively, enveloping negative mesenchymal cells derived from paraxial mesoderm.
3rd branchial arch
detected detected
single cellCRABPI is expressed in two broad subectodermal sheets of neural crest cells arising from rhombomeres 4, 6 & 7; they colonize the periphery of the 2nd, 3rd & 4th arches, respectively, enveloping negative mesenchymal cells derived from paraxial mesoderm.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:14729
Entity Detected:Crabp1, cellular retinoic acid binding protein I ( MGI:88490)
Sequence:sense strand is shown

>MGI:14729
CCAGCGTGCGACCGCTCCGCAGCAGAGGTGGGTGCCTGCCCCTGCCTTCGCGCCGCCGTACAGCCAACAC
CACTGCCACCATGCCCAACTTCGCCGGTACCTGGAAGATGCGCAGCAGCGAGAATTTCGACGAGCTCCTC
AAGGCGTTGGGTGTGAACGCCATGCTGAGGAAGGTGGCCGTGGCGGCTGCGTCTAAGCCGCACGTGGAGA
TCCGCCAAGACGGGGATCAGTTCTACATCAAGACATCCACTACTGTGCGCACCACGGAGATCAACTTCAA
GGTCGGAGAGGGCTTCGAGGAGGAGACAGTGGACGGACGCAAATGCAGGAGTTTACCCACGTGGGAGAAT
GAGAACAAGATTCACTGCACACAGACTCTTCTGGAGGGGGATGGCCCCAAAACTTACTGGACCCGAGAGC
TGGCCAACGATGAGCTCATCCTGACATTTGGCGCCGATGATGTGGTCTGCACGAGGATTTATGTCCGGGA
GTGAAAGGTGGCCAGCTTGTTCCTGCTTCATGACGATGCGAGTTCCCCTGAGGGGTATGCCGTGGCCCCA
CACTGCCAGTGGGTCTTTACTCCACACACCTCTCCCCCATGAATATTAGGCAACCCCATTTTCCCCATGA
CATTGTTGTAGTGTCCTCCCCTCAGGCTCTTGTTGCCTTGTGTACTGTGTACCCTTGTTTGGCATTTGCA
TGATGTACCAGTCATTAAACTGGTTGGCTGC
nt 1 - nt 731 of X15481.1
Notes:The probe used in this study by Dupe et al., 1999 [PMID:10529422] is indicated as being described by Dolle et al, 1990 [PMID:2556642] ie. as the "CRABP full length cDNA (Stoner & Gudas, 1989 [PMID:2538228] - see Fig1B in Stoner for sequence). The probe includes an additional poly(A) tail not included in the sequence link above.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount plus section of wholemount
Procedures
General Information
Authors:Dupe et al., 1999 [PMID:10529422] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10529422] Dupe V, Ghyselinck NB, Wendling O, Chambon P, Mark M 1999 Key roles of retinoic acid receptors alpha and beta in the patterning of the caudal hindbrain, pharyngeal arches and otocyst in the mouse. Development (126):5051-9
 [ doi:10.1038/342702a0] [ PMID:2556642] Dolle P, Ruberte E, Kastner P, Petkovich M, Stoner CM, Gudas LJ, Chambon P 1989 Differential expression of genes encoding alpha, beta and gamma retinoic acid receptors and CRABP in the developing limbs of the mouse. Nature (342):702-5
 [ PMID:2538228] Stoner CM, Gudas LJ 1989 Mouse cellular retinoic acid binding protein: cloning, complementary DNA sequence, and messenger RNA expression during the retinoic acid-induced differentiation of F9 wild type and RA-3-10 mutant teratocarcinoma cells. Cancer Res (49):1497-504
Links:MGI:1346588 same experiment
 EMAGE:240 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI