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EMAGE:23916

hs269 VISTA human element 269
TS18 (11.5 embryonic day)
regulatory element reporter

Data Images
EMAGE:23916 EMAGE:23916 EMAGE:23916

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Images reproduced with permission from the VISTA Enhancer Browser. Element hs269 - embryo 2.
Expression Pattern Description
Spatial Annotation:
EMAGE:23916Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
23916_wholemount_strong.wlz
23916_wholemount_moderate.wlz
23916_wholemount_weak.wlz
23916_wholemount_notDetected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:23916_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
forebrain
detected detected
VISTA annotation: forebrain (10 out of 12 embryos)
hindbrain
detected detected
VISTA annotation: hindbrain (rhombencephalon) (10 out of 12 embryos)
midbrain
detected detected
VISTA annotation: midbrain (mesencephalon) (10 out of 12 embryos)
Annotation Validation: EMAGE Editor
Detection Reagent
Type:regulatory element reporter
Entity Detected:hs269 VISTA human element 269
Sequence:chr5:90928612-90929226  (UCSC human hg19 )
>hs269
TATTGGAATTCAGTATTTATTTTGGCATTTAACAAAGTAATAAATATTTCTCTATATTCTGCTTAAGTAA
TGGAACAAATAGGACTCAATATATTACAAGGTTTAAATTATAATATTTTTAAAAAGCAACAGTGTGTGCC
TCAAGTGTTGCAAACTTGATTTGAGCATTTTATGGTACTCCAGTCAGCTGCCTATTAAAAGTGTGATCAG
GTAGTTTAGACTGTGACTAGCTGTGTCAACAGAAGGAAAGTGGAGGATTAGTCTCTATGGCAACGACAAA
GTATCCCTGTTTGTAGACAGACAAATTCTGCTACATCTGAAACTGATTGACAGGTCTATTCCTTTGCAAC
TGACCCATAGAAATTAGCAAGTAAAAGTTATTATACTGGGTTGTATTGAAGGGTTCTCAATATCTTAAAG
TGCTCTTGTAAGTTTTTCTGCAGCATTTGCATGAGAAACCTGGCAGGAATTGTTCTGAGGTTTATATCTA
TGGTTTGATAGATTCTTTGTGGGGGAGTATTGCTGGTGAGAATAACTTAATTTTCAGTTCTCTGTATGAC
AGAATTGTTGGAGCATGGATTGAGGGGAAAACACACATCAAAGACCCAAAGCTGA
(Equivalent mouse sequence - chr13:80762168-80762775  (UCSC mouse mm9 ))
Flanking Genes:ARRDC3 - NR2F1 (human)
Primers:Vector specific   fwd:(+)CACCGGAATTCAGTATTTATTTTGGCATTT rev:(+)TCAGCTTTGGGTCTTTGATG
Vector:Hsp68-Lacz
Labelled with:LacZ
Promoter:Hsp68
Specimen
Organism:mouse
Strain:FVB
Age:embryonic day 11.5
Theiler Stage:TS18
Mutations:Tg(hs269-Hsp68-lacZ)
Preparation:wholemount
Procedures
Fixation:4% PFA (paraformaldehyde)
General Information
Authors:Pennacchio et al., 2007 [PMID:17130149] . Spatially mapped by EMAGE.
Principal investigator:Len Pennacchio, Genomics Division Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Mailstop 84-171, Berkeley, USA CA 94720
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1093/nar/gkl822] [ PMID:17130149] Visel A, Minovitsky S, Dubchak I, Pennacchio LA 2007 VISTA Enhancer Browser-a database of tissue-specific human enhancers. Nucleic Acids Research (35):D88-92
Links:hs269 VISTA human element 269 same experiment
Data SourceVISTA