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EMAGE:24085

mm87 VISTA mouse element 87
TS17 (11.5 embryonic day)
regulatory element reporter

Data Images
EMAGE:24085

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Images reproduced with permission from the VISTA Enhancer Browser. Element mm87 - embryo 1.
Expression Pattern Description
Spatial Annotation:
EMAGE:24085Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
24085_wholemount_strong.wlz
24085_wholemount_moderate.wlz
24085_wholemount_weak.wlz
24085_wholemount_notDetected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:24085_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: EMAGE Editor
Detection Reagent
Type:regulatory element reporter
Entity Detected:mm87 VISTA mouse element 87
Sequence:chr10:44948419-44951592  (UCSC mouse mm9 )
>mm87
TTCCTTGAGAGTGGCTTTCAGCATCTTTCTGTCTCATTGTTTCTTTGACTATAAAATGAAGCTTTTGAAT
GGAATATAGCTTGGTGGTATAGTGCTTATCTAGCAGGTGTGGAGCCCTGGGTTCGATCTCTAGTTCTGTG
GGAAGAGGGAAGAGAGATCATCAATAATGCTGCGCCATCCCAGGAGTTCTGCAAGGATGGTATGGAACCC
GGGATGATCCTTGAACAGTGTCCGGTTCTTAGTCAGCCCACCGTAGATCAGGGCCATTGTTGCTGCTGCC
ATCCTTGCCCCTACTTTTCTCACCACCATGATTATCTGAAGGTCAAGGAGGAGGGACTTATCTACATCCT
TTGATTTGGAATCCTGAGAAAATGTATCCTACCTTCCTACAAGGCTGCTACCTTCATAAAGCATCTGCAT
GAAGAGCCAATTCCCAGCTTCCCTCCCTAAACATGAATGTTACGTAAAATCTGCTTAGGCTAAAAGGAAG
GCTTAATTTCAAGTTATATTCAAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTGTGAGACAGGGT
TTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCGAACTCAGATATCCG
CCTGCCTCTGCCTCCCAAGTGGTGGAATTAAAGGTGGGCACCACCACTCCCTGCCCTATATTCAAGCTTT
ATCTTACATTAAAATTCTTCATTTCAATGAAGGTGTAAAACTGCTAGACTGAGCCAAGGTCTACCTGGAA
AATCATTAGTCCCTAAGCTCTGGGGGAGACACTGAGGGAGAATACACACATGTAATTTTTCTCAGAGAGC
TTCCTCATATATGCATCTGTTAAGCAATACTGAGCCAGACCGCGTTTTTAGTGACCTCTTGCCCTTCTAT
CCCGTTGGCTAAAGCAGAGACTATCATTCTAGTTTGAGTGAATTCTGTTTATTTACTAGACTATTGAGAA
GAATGAAGGGAAATGAAATTTTCCTTCAGTGAATAAGCGGCACATTTTCAGTCAGGTAAATAAAAATGGA
AGAGCAAATATCATCAGTCAAGAACTGTAAGATTTCAGTCAGTATTCTTGAGCTATTTGACCACCTAATG
CTGCTATTTGTCTGCTTAGTAAACTAGGGCTTTATCGTGAATGTACTGGGATCATTATTCAACTCCCAGA
CTGCCGGTGCCTGCCTGGCCTTTATTTAAAGTGACACAATTATCAGATTGGAGAATCTGCCACGCCATTG
ACTCAGCCCAGCAGAACACGAAACTGAGTTACAAGCATTAATTTGCTCTCACCAAGAGACAGGAAGCATT
AAACAAGTCGAGTTCGAAGCAACATAATCTAGTCATCTCATTCACTTCAAAGTCAGCAGAATGGAAAGAG
AAAGTCTGCGACTGACCACTAAATAGGCCACCTCTGCACTCAGAAATTCCTGGAGAGCGTTTCAAGTGTT
CTCTGAAGGAAAGAATGCTGTGAAGTTCAGCTCTGGAAAGCCTCCGGGACACTTCTGAATGCACCAGACT
TGATTGGGTGACACTAGAGGTCCCCCAGAGGCATGTGAGGAGGTTCAGAGAGATTAATGTGCAGGGGGCT
GGGATGTGTCTGCCAACATCTGCTTCTCACTCTTGATAGTAACAGTTTGTGACTGTATCCACTGCTGCCT
GGGGAGATCACATTTCCCAGCTGCCCTTGCAGTTTGATGTGGTCTTTGAAGTTTTTAGAGGAGTAATGTG
AGGAAAAGTGATACTTACCACCTTCAGGTCTGGGACTGAAGACATCAAGAGGAAATTCTCAGATTCACTC
ACAGTTTCTAAAGACAAGAACACAATGACAGTCCTAACTATAGCAATGCAGAAGATGGGAACACCTTAGG
GTCTCACGACTTCAGGGTTCACCCAGGAAGCATTAGAAAGACACAATAAAAGAAAGACTTTACCCAGGAC
CTACTAAAGCAATCTGCATTTTACTTAGGTCCTTATGGGAATCCAATATGCATTCAATTCTGAGGCACAC
AGGCCAGAGGATGGCAGAGCTGTCATGAAACTGGGTCTCCATTTAAGCAGAGATGTACCTCAGAGTGTAA
TGCTCACTCTGTGATACATGAGAAAGGCGACTGTGAAGTCAATTTGTTGCTGGTGCTTAAATTTATCAGA
AACCAAATAGTGTGGCGGAATTCACAGTGTCTTGTTTACCAGAATATACTAGCAACTTCCCATGGGATAC
CTGTCCAGAGCTAACCAGTCTGCTGAAGTTCTGCCACTATGTGAGGATTCTAGAAGGTTCTGCTGCTCAG
AAGTCATTGATAAAGTGGGGAGCTGGGGATGGGGACGAGAGTTCTGAATGGGTAGGTGCTTGACTCCAGG
CTGGAAAACTAGAGGAATTAAAATAACTGAGAATCAGCTCTGCTCCCAGAATGCCCCAGCCATTCCTGGC
AGCTGCAACAAAGCTTCAGGCTCCTTCTCTGCAGCCCTTGCCTGCAGCCAGCCCTAGGGCTCACTGGCCG
AGTCAGAGCACTGCCCTTCCTATCCTAGCCCAGAGTGGAATTACGATCAGCATTAACTCTGTGAAGGAAA
TGACAAACTTACATTCCTAAGAATTCCCACAAGCTCGGTTACCTGGGGAGGTGCATGAGTTCATCAGACA
CCTGCATGTGTGCCAGAGATGTTCAAGGTCCTCTGTCACCCAGAAAGCAAGGACGTGTAGCATCACATCA
GAGACAGGCAGGGGCATCTCCTACTGACAATGCTGAGGACAAGGCAGATATTTATTATAAATGCATGTTT
ATGTGCCAGGTAAACTTCAATGGAGTAAAATCAATTGAGCTCTTAAAAGTCATGGTTTCAGGGTGTTCAC
ATGCATGAGGTTGTGCCTCCGGTGTCTTTCCTCAGGCAGTGTCCTCTGTTTTTTGAGATAGGTCTCGTAT
TGGCCCGAGGTTCACTGATTTGGTTAGACTGGCCCGGCCAGTGAGCCCTAGAGATCTTTCTATCTTTGCC
TCTCCAATGATAGGATTAAAAGTGTGTGCCACCGTGCCAGGCCTTTTTATGTGGGTTCTGGGGAATCGAA
TTTGGGTCCTCACGCATATACAGCAAGCACTTTACTGACTGAGCATCTCCTGAGCCCTGAAGCCATGGAT
TTGTGATACTAGGCCCAACTCCCC
(Equivalent human sequence - chr6:105673389-105675851  (UCSC human hg19 ))
Flanking Genes:Prep - Popdc3 (mouse)
Primers:Vector specific   fwd:(+)CACCTTGAGAGTGGCTTTCAGC rev:(+)GGGGAGTTGGGCCTAGTATC
Vector:Hsp68-Lacz
Labelled with:LacZ
Promoter:Hsp68
Specimen
Organism:mouse
Strain:FVB
Age:embryonic day 11.5
Theiler Stage:TS17
Mutations:Tg(mm87-Hsp68-lacZ)
Preparation:wholemount
Procedures
Fixation:4% PFA (paraformaldehyde)
General Information
Authors:Pennacchio et al., 2007 [PMID:17130149] . Spatially mapped by EMAGE.
Principal investigator:Len Pennacchio, Genomics Division Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Mailstop 84-171, Berkeley, USA CA 94720
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1093/nar/gkl822] [ PMID:17130149] Visel A, Minovitsky S, Dubchak I, Pennacchio LA 2007 VISTA Enhancer Browser-a database of tissue-specific human enhancers. Nucleic Acids Research (35):D88-92
Links:mm87 VISTA mouse element 87 same experiment
 EMAGE:23919 same experiment
 EMAGE:23973 same experiment
 EMAGE:24017 same experiment
 EMAGE:24108 same experiment
 EMAGE:24179 same experiment
 EMAGE:24288 same experiment
Data SourceVISTA