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EMAGE:24262

mm182 VISTA mouse element 182
TS18 (11.5 embryonic day)
regulatory element reporter

Data Images
EMAGE:24262 EMAGE:24262

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Images reproduced with permission from the VISTA Enhancer Browser. Element mm182 - embryo 4.
Expression Pattern Description
Spatial Annotation:
EMAGE:24262Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
24262_wholemount_strong.wlz
24262_wholemount_moderate.wlz
24262_wholemount_weak.wlz
24262_wholemount_notDetected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:24262_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
heart
detected detected
VISTA annotation: heart (4 out of 4 embryos)
Annotation Validation: EMAGE Editor
Detection Reagent
Type:regulatory element reporter
Entity Detected:mm182 VISTA mouse element 182
Sequence:chr2:113949124-113949618  (UCSC mouse mm9 )
>mm182
AAGTGACAACCTGGGAAACAAAGCAGTTAACAAGTCAAATGAAAACAGACTGCGTGACTGCAACTCCACA
CTGTCACCACACTGACAGAATTGAGGGTCATCTGAATGTCAGAGGCGACATGAGCTTCAGCAACAAGGCC
TTCTATGGCTATTTCTGTGACAGGGCTTCTCCAGGACTGCTGCACAATCCCACCCTAAGTGGTTTGCACT
GAACAGGTCCTATCAAGGCATGGGACAAAAGTCAGTCAGAGCCTGGGAGATAATGCATGACTTACAGATC
GTGCATCTTAAAAACTAACTATTAGCTTTGATTGTACAATAAACTTCATTAATTAGAAAGAAGAAAAAAA
AAAGTTCTAGTACTTTGTTTTATTGCAATTATTCAGGGGAAGAACAAATTAACATTCTTCCCAAATCAAT
AGGTCAACAAATGTTTGGCATATTGTACAAGCTACCACTATTTATAAATTTCAAAACCACTGCTAAAGCA
TTCCA
(Equivalent human sequence - chr15:35183632-35184148  (UCSC human hg19 ))
Flanking Genes:Aqr (intragenic) (mouse)
Primers:Vector specific   fwd:(+)CACCGACAACCTGGGAAACAAAGC rev:(+)TGGAATGCTTTAGCAGTGGTT
Vector:Hsp68-Lacz
Labelled with:LacZ
Promoter:Hsp68
Specimen
Organism:mouse
Strain:FVB
Age:embryonic day 11.5
Theiler Stage:TS18
Mutations:Tg(mm182-Hsp68-lacZ)
Preparation:wholemount
Procedures
Fixation:4% PFA (paraformaldehyde)
General Information
Authors:Pennacchio et al., 2007 [PMID:17130149] . Spatially mapped by EMAGE.
Principal investigator:Len Pennacchio, Genomics Division Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Mailstop 84-171, Berkeley, USA CA 94720
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1093/nar/gkl822] [ PMID:17130149] Visel A, Minovitsky S, Dubchak I, Pennacchio LA 2007 VISTA Enhancer Browser-a database of tissue-specific human enhancers. Nucleic Acids Research (35):D88-92
Links:mm182 VISTA mouse element 182 same experiment
 EMAGE:23963 same experiment
 EMAGE:24221 same experiment
Data SourceVISTA