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EMAGE:24370

mm429 VISTA mouse element 429
TS19 (11.5 embryonic day)
regulatory element reporter

Data Images
EMAGE:24370 EMAGE:24370

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Images reproduced with permission from the VISTA Enhancer Browser. Element mm429 - embryo 5.
Expression Pattern Description
Spatial Annotation:
EMAGE:24370Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
24370_wholemount_strong.wlz
24370_wholemount_moderate.wlz
24370_wholemount_weak.wlz
24370_wholemount_notDetected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:24370_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
detected detected
VISTA annotation: branchial arch (6 out of 6 embryos)
head mesenchyme
detected detected
VISTA annotation: facial mesenchyme (6 out of 6 embryos)
genital tubercle
detected detected
VISTA annotation: genital tubercle (6 out of 6 embryos)
limb
detected detected
VISTA annotation: limb (6 out of 6 embryos)
Annotation Validation: EMAGE Editor
Detection Reagent
Type:regulatory element reporter
Entity Detected:mm429 VISTA mouse element 429
Sequence:chr5:38358862-38360435  (UCSC mouse mm9 )
>mm429
AGCCTCTGGAATTGGGAAGAAATTCCTTGCAAATTCCATGGCCACCCCTGACCAGTGAGAAAATGAGAGT
GCAGCCCAGCTCGACGGCGCGGTGCAGCCTGGCTCCATTAAATGAGTTCAAAACAATTACCAGGGAACAT
GTGTTTCCTATGAAGGGAGCAAGTGTTAAAACGGATTTAATTTTCTTTTGTAAATAAAGAGGCCTATCCA
GCCCGGCCTGTTTATCTCCCTGAGAGAGATGAAAGTTGTTTCATCTGCCGGTAGCCTCTGAGCGCCTCCC
CACCGAGGCGGGGCCGCTATCTGTACTCCATGTGTTGTGTATCTGTAGAGCCTTTCCATCGCGGTCATGT
GGAACTGTTTGAGCACCATGAGGTCTCTTCTCAAAGTCTGACTCTTAGTTATCTACAATGGCGCTCCTCT
CCCTCTCCCTTAAACATATTAGTGATTCATAAACAGTAAAATTAGTTGGCTGGGGAGGGGTTGGTGGAGG
TATTCATTCTGGCGGCGCCTGCTTTCCTGGGTTGGTAGTTAAAATAAATCATGGCTGTCACAATGGAATT
CGGGCCTAAAAATCACCTGTAGGAATCCCCCACACGCACACCCCGCACCCACGTTCCCCACCCCCACCCC
CAGCATGGATGATCAAAGCTACCCTACTCCAGCTGGAGAAGGGGAGTGAGATTCGGGCGAGGACGAGACA
CGGAGGAGAAAGACAAGAGGCAGTTTCAACAGTCAGAAGGAAACATCCAGAAGTTATGAGGAAGGAATCT
GGAAATTAACATCTCTGCTCTGGAACTGCACGAGAATGAGGTTGACTTAAGATCTTAGTTTTTCTAATAC
AAAATATAATTAATCACTTCGTTCCGTGAAGTTGTGTTTCCACTTGACCTACCCGCGCTGGCAGCCAGAA
CTCAGGTTTGCTTTTTTGTGTTTCTTTTTTCCCCGACTGCAAATTTAATAAATTTCAACATGCCCAAACT
TCCTGACTTACACTGTCCACCACGGGTATCTCTGGAAACTTCAATAGCTGGAGATAACTCACACACTCTG
CTCCGCCTACCTTCTCGGCGCTCACAGTAATCCGGGATCAGGCGACTAAGCTTTCATCTTGAGAGCATTT
AAGTTAATTACACGAGGCCAGCAAGGCCCTGAGGAGCCGGTTCTGCGCAGCCCTCCTGAAGCCAGACATC
ATAGGATCCAGATGCAACCCCTTGGCAACTTAAGCCCAACAAATTTCCTTTGTCAAGAAATGCAAACAGC
CCAACATTGAAGCCCAGGCTACTACAGTGAGTCGGCTTCCTTGGGGCTAGACTACAGCTCCCAGAGCCAC
CATCCAGGCCAGAAGCTATTGAAAGGTGCAAGTGCCCACTTAGCGGCACCTTCCACCCTCCCACCTGGGT
ACGGGTTACCAAGGATGGCTCATTTTAGTTTGAATGGGTCGCTGATCAGAACATGACTGCCTCTTGTCAG
TTGACCCCAATCCAAGGCATTTTCAGGACAGTGCATTTACTGGCAAGAGACTGTCCCACTTAGTTGGCAG
GTTTCCTACTGTGAACTTTGTTCCCAGATGTCTG
(Equivalent human sequence - chr4:4667671-4669193  (UCSC human hg19 ))
Flanking Genes:Msx1as - Stx18 (mouse)
Primers:Vector specific   fwd:(+)cacCTCTGGAATTGGGAAGAAAT rev:(+)CAGACATCTGGGAACAAAGT
Vector:Hsp68-Lacz
Labelled with:LacZ
Promoter:Hsp68
Specimen
Organism:mouse
Strain:FVB
Age:embryonic day 11.5
Theiler Stage:TS19
Preparation:wholemount
Procedures
Fixation:4% PFA (paraformaldehyde)
General Information
Authors:Pennacchio et al., 2007 [PMID:17130149] . Spatially mapped by EMAGE.
Principal investigator:Len Pennacchio, Genomics Division Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Mailstop 84-171, Berkeley, USA CA 94720
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1093/nar/gkl822] [ PMID:17130149] Visel A, Minovitsky S, Dubchak I, Pennacchio LA 2007 VISTA Enhancer Browser-a database of tissue-specific human enhancers. Nucleic Acids Research (35):D88-92
Links:mm429 VISTA mouse element 429 same experiment
 EMAGE:24367 same experiment
 EMAGE:24368 same experiment
 EMAGE:24369 same experiment
 EMAGE:24371 same experiment
Data SourceVISTA