EMAGE mouse gene expression spatial database

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EMAGE:24467

mm622 VISTA mouse element 622
TS18 (11.5 embryonic day)
regulatory element reporter

Data Images

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Images reproduced with permission from the VISTA Enhancer Browser. Element mm622 - embryo 5.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

24467_wholemount_strong.wlz

24467_wholemount_moderate.wlz

24467_wholemount_notDetected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:24467_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

branchial arch

detected

VISTA annotation: branchial arch (9 out of 11 embryos)

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	regulatory element reporter
Entity Detected:	mm622 VISTA mouse element 622
Sequence:	chr5:112030282-112031759 (UCSC mouse mm9 ) >mm622 CACAGCCTTTGAGACGACATAACTGTTTCCATAGATAAAACCAACGTCACTCTAACATAAGGGCCCCTCT GGGGGAGTTTTGACTGCATATTTGGCACCAACTGGTGCTTACCCCCAAACCCTGCTTCCCAAACCCCATC CATTCCAAAGAGCCCTGGGTCCTAAGGGTACATTCAGCCCACGGAGAGCCTCCAGAGTCTCTCCTTAATC TACTGGAACAGGTCCCAAGCAGACTCCTCAGTTGTCTCCTTTGGGGTTCATCACGGTTGACAAAGTACCT CCATTTCCTCTGCCCCAAATATATCTCCTCAGTCTAGAAACCTTGACAGGCAAGATCATTTCTCCTTTGC TGGGGTTCAGATATCACTGACAGCTGGGTGGGGGACTAGAGGTGACGTGGTCACATTGAGGTATCTGTCA TACTAGGGGGCAAAGCCTGGTGGGACAGATTGTCTGAAGGGCAGATGGTGGTGGCTGGTAGGAGAGAGTA GAAGATTACATCCACCTTAGCACAGAGTGAGGCCCTGGGCTTTGTAGGAGGCTCGGCCTTGCTGGGAGCA AGCCCAGAAAGGCAGGGTTCACAGGCAGGGCGGTCTTGACAACGGTAACCCCAAGGCCAAAGGCAGAGGA AAGAAAAGAACATTCCATGATGTATATATATTTAGGAGGCCGGGAGACAGGAAAGGAAATCAAGTTAGAG ACCGAGAGTTCGCAGCCTGAGCAGGTCTCCAGGCCCCGGGAGAGGTTGTGGAATCCAGATGTGGTCGGGA AGGCGGAGGGGGAGGGGGAGGGGAGGAGGGGAGGGGGGGGGGCAGGCCTGTTCACAATTAGCCTTGATTT TGTGTGATGACATTCAAATTGTGGGCAAGCATGAAAGCAGAGTTGTGAGGACGCGGCTGTCCGACGGCCC CGGGACTAATCCAACGTGAAGGGCTCGGGCGCAGGCCTGGCACCACATGGAAGTACTTTGGGAGAGTTTG ATGTTCCGCCAGCTAAAGGGGGATCCATGGTCCTGGCTGAGTGGGCCTCCTCCTCGCCCCGCCTCCTCAG GACTGGCTGGCTCTGGCGAGAAGGGTTAAACCTCGAACACACCAGAAGGTTCTGGGCCTGTGGATGCCCT CACTAGCCCAGCCCTGAAGGCAGCAAGGTGGTCATGTGAGACCCACAGAGCCGGGCCTTGGGCGCTGGGC CAGCAGCAGTGGCTGACGTGGTGTGGGATGGTGTCGTCTGTGAGCTATTTTCAATATTTCTAATATTACA AAGATGAGGTAGCCCACATACTGGGACTTTGGCAGGCAACAGCTTTTGTAACAGGGCCCGTCAGATGGGC CTTGGCTGCAAATTTTGGGATTTTATAGGAAACTGGGGGGGTGGTACAGGAGGCCATCTGGGGCCTTGGG TCACCTAGCAAAAAGGTCCAGGCTCACAGGGAAGCCATCTCGTGAAATGAATTCAGAGAGAGTTGTCATT CCATCGTC
	(Equivalent human sequence - chr22:27934200-27936053 (UCSC human hg19 ))
Flanking Genes:	C130026L21Rik - Miat (mouse)
Primers:	Vector specific fwd:(+)caccGCCTTTGAGACGACATAACT rev:(+)GACGATGGAATGACAACTCT
Vector:	Hsp68-Lacz
Labelled with:	LacZ
Promoter:	Hsp68

Specimen

Organism:	mouse
Strain:	FVB
Age:	embryonic day 11.5
Theiler Stage:	TS18
Preparation:	wholemount

Procedures

Fixation:

4% PFA (paraformaldehyde)

General Information

Authors:	Pennacchio et al., 2007 [PMID:17130149] . Spatially mapped by EMAGE.
Principal investigator:	Len Pennacchio, Genomics Division Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Mailstop 84-171, Berkeley, USA CA 94720
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1093/nar/gkl822] [ PMID:17130149] Visel A, Minovitsky S, Dubchak I, Pennacchio LA 2007 VISTA Enhancer Browser-a database of tissue-specific human enhancers. Nucleic Acids Research (35):D88-92
Links:	mm622 VISTA mouse element 622 same experiment
	EMAGE:24463 same experiment
	EMAGE:24464 same experiment
	EMAGE:24465 same experiment
	EMAGE:24466 same experiment
	EMAGE:24468 same experiment
Data Source