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EMAGE:264

Zic3 zinc finger protein of the cerebellum 3 ( MGI:106676)
TS14 (9 dpc)
in situ hybridisation

Data Images
EMAGE:264
Fig 1C of Purandare et al., 2002 [PMID:11959836] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:264Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
264_wholemount_strong_3D_1.wlz
264_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:264_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
head somite
detected detected
tail bud
detected detected
central nervous system
detected detected
trunk somite
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2180326
Entity Detected:Zic3, zinc finger protein of the cerebellum 3 ( MGI:106676)
Sequence:sense strand is shown

>MGI:2180326
TCTAGATTCCTTACATTGTCAGTTTCCATCTGGGGAGGACCCCTCCTGGCTTTATCTCTAGCTCAGATGG
TTGTTTAACTGGGAGTTTAAATGTGTTTGTCCTGGATTTTCGGCATGCAAATCAAATAATACTGATCAAT
TCACTTAGTGGCCATGACATCTCAATCTTGTACTTCAAAGACTGAGAGGCTGGGTTTAATCATTCCTGCC
CTATATATATATATACATATATATATAAACAGAAGGTAACCTAATTAGCTTTATGTCCCTTAGTTCTTTA
TTACTTTACATAAAAATGAAAATTATGGCAGGATGCATGTCTGTTGAGAGATCACTCATATACCTATATG
TTTGTATCTATGACGTACCTAATCTGCCTATCAAATCTATTTAGCTTCAAAGGATAGCACACCTCTGAAA
TGCCAATGACCAAGGCCAGTTGAACAGTGCTTCTCATGGTTAAAGTGCTTCT
nt 2825 - nt 3296 of NM_009575.1
Notes:The probe used in this study by Purandare et al., 2002 [PMID:11959836] is described as " spanning 471bp in the 3' region of the gene and was generated using PCR. The primers used were F 5'-TCTAGATTCCTTACAATGTCAGT-3' and R 5'-AAGAAGCACTTTAACCATGAG-3'."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Purandare et al., 2002 [PMID:11959836] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11959836] Purandare SM, Ware SM, Kwan KM, Gebbia M, Bassi MT, Deng JM, Vogel H, Behringer RR, Belmont JW, Casey B 2002 A complex syndrome of left-right axis, central nervous system and axial skeleton defects in Zic3 mutant mice. Development (129):2293-302
Links:MGI:2180827 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI