EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:279

Wnt7a wingless-related MMTV integration site 7A ( MGI:98961)
TS15
in situ hybridisation

Data Images


Figure 1H of Loomis et al., 1998 [PMID:9463360] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.	Figure 1G of Loomis et al., 1998 [PMID:9463360] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:
Find spatially similar expression patterns:

Notes:

Image 1G is a wholemount image which was subsequently sectioned sagittally to produce image 1H. Image annotations: d - dorsal; v - ventral. Limbs were staged as described by Wanek et al., 1989 [PMID:2926360] . Both 1H and 1G are Stage 1. It is not specified whether the images are of fore- or hind-limbs, but can be seen from wholemount view this is a forelimb.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

3D mapping

3D context movie

wholemount mapping

View mapped 3D expression image

Download individual expression domains:

279_wholemount_strong_3D_2.wlz

279_wholemount_notDetected_3D_2.wlz

279_voxel_strong_3D_1.wlz

279_voxel_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:279_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Find spatially similar expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb bud ectoderm

detected

regional

Expression is in the distal and dorsal ectoderm covering 2/3 of the limb.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:9297
Entity Detected:	Wnt7a, wingless-related MMTV integration site 7A ( MGI:98961)
Notes:	The probe used in this study by Loomis et al., 1998 [PMID:9463360] is indicated as being that used by Parr & McMahon, 1995 [PMID:7885472] which was previously described by Parr et al., 1993 [PMID:8275860] as a "400bp SmaI-EcoRI (coding and 3' untranslated)" fragment.
Chemistry:	RNA
Label:	digoxigenin

Specimen

Organism:	mouse
Theiler Stage:	TS15
Mutations:	none (wild-type)
Preparation:	wholemount plus section of wholemount

Procedures

Fixation:	4% paraformaldehyde
Embedding:	cryosection
Staining procedure:	alkaline phosphatase

General Information

Authors:	Loomis et al., 1998 [PMID:9463360] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:9463360] Loomis CA, Kimmel RA, Tong CX, Michaud J, Joyner AL 1998 Analysis of the genetic pathway leading to formation of ectopic apical ectodermal ridges in mouse Engrailed-1 mutant limbs. Development (125):1137-48
	[ doi:10.1002/jez.1402490109] [ PMID:2926360] Wanek N, Muneoka K, Holler-Dinsmore G, Burton R, Bryant SV 1989 A staging system for mouse limb development. J Exp Zool (249):41-9
	[ PMID:8275860] Parr BA, Shea MJ, Vassileva G, McMahon AP 1993 Mouse Wnt genes exhibit discrete domains of expression in the early embryonic CNS and limb buds. Development (119):247-61
	[ doi:10.1038/374350a0] [ PMID:7885472] Parr BA, McMahon AP 1995 Dorsalizing signal Wnt-7a required for normal polarity of D-V and A-P axes of mouse limb. Nature (374):350-3
Links:	MGI:1339621 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source