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EMAGE:288

Wt1 Wilms tumor 1 homolog ( MGI:98968)
TS20 (12.0 dpc)
in situ hybridisation

Data Images
EMAGE:288
Fig 5A of Mendelsohn et al., 1999 [PMID:10021334] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
Image annotations : ub - ureteric bud
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
cap mesenchyme
detected detected
regionalExpression was detected in peripheral and condensing metanephric mesenchyme surrounding the ureteric bud tips.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:15948
Entity Detected:Wt1, Wilms tumor 1 homolog ( MGI:98968)
Notes:The Wt1 probe used by Mendelsohn et al., 1999 [PMID:10021334] is described as: "WT-1 (Kreidberg et al., 1993 [PMID:8395349] ) was linearized with Not1 and a 1.4 kb transcript was synthesized with T3 polymerase". Kreidberg et al. describe a Wt1 targeting vector.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:12.0 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Mendelsohn et al., 1999 [PMID:10021334] Indexed by GXD.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:3897439] Doetschman TC, Eistetter H, Katz M, Schmidt W, Kemler R. 1985 The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. J Embryol Exp Morphol. (87):27-45
 [ doi:10.1016/0092-8674(93)90515-R] [ PMID:8395349] Kreidberg JA, Sariola H, Loring JM, Maeda M, Pelletier J, Housman D, Jaenisch R 1993 WT-1 is required for early kidney development. Cell (74):679-91
 [ PMID:10021334] Mendelsohn C, Batourina E, Fung S, Gilbert T, Dodd J 1999 Stromal cells mediate retinoid-dependent functions essential for renal development. Development (126):1139-48
Links:MGI:1330551 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI