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EMAGE:290

Wnt7a wingless-related MMTV integration site 7A ( MGI:98961)
TS17
in situ hybridisation

Data Images
EMAGE:290
Figure 1I of Loomis et al., 1998 [PMID:9463360] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: d - dorsal; v - ventral. Limbs were staged according to Wanek et al., 1989 [PMID:2926360] . This is a stage 3 limb bud.
Expression Pattern Description
Spatial Annotation:
EMAGE:290EMAGE:290Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
290_voxel_strong_3D_1.wlz
290_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:290_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
forelimb bud ectoderm
detected detected
regionalExpression is restricted to the dorsal region.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:9297
Entity Detected:Wnt7a, wingless-related MMTV integration site 7A ( MGI:98961)
Notes:The probe used in this study by Loomis et al., 1998 [PMID:9463360] is indicated as being that used by Parr & McMahon, 1995 [PMID:7885472] which was previously described by Parr et al. 1993, [PMID:8275860] as a "400bp SmaI-EcoRI (coding and 3' untranslated)" fragment.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount plus section of wholemount
Procedures
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Loomis et al., 1998 [PMID:9463360] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9463360] Loomis CA, Kimmel RA, Tong CX, Michaud J, Joyner AL 1998 Analysis of the genetic pathway leading to formation of ectopic apical ectodermal ridges in mouse Engrailed-1 mutant limbs. Development (125):1137-48
 [ doi:10.1038/374350a0] [ PMID:7885472] Parr BA, McMahon AP 1995 Dorsalizing signal Wnt-7a required for normal polarity of D-V and A-P axes of mouse limb. Nature (374):350-3
 [ doi:10.1002/jez.1402490109] [ PMID:2926360] Wanek N, Muneoka K, Holler-Dinsmore G, Burton R, Bryant SV 1989 A staging system for mouse limb development. J Exp Zool (249):41-9
 [ PMID:8275860] Parr BA, Shea MJ, Vassileva G, McMahon AP 1993 Mouse Wnt genes exhibit discrete domains of expression in the early embryonic CNS and limb buds. Development (119):247-61
Links:MGI:1339621 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI