EMAGE mouse gene expression spatial database

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EMAGE:29198

Smarca2 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 ( MGI:99603)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000706_12	euxassay_000706_01	euxassay_000706_02	euxassay_000706_03	euxassay_000706_04

euxassay_000706_05	euxassay_000706_06	euxassay_000706_07	euxassay_000706_08	euxassay_000706_09

euxassay_000706_10	euxassay_000706_11	euxassay_000706_13	euxassay_000706_14	euxassay_000706_15

euxassay_000706_16	euxassay_000706_17	euxassay_000706_18	euxassay_000706_19	euxassay_000706_20

euxassay_000706_21	euxassay_000706_22	euxassay_000706_23

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Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T776
Entity Detected:	Smarca2, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 ( MGI:99603)
Sequence:	sense strand is shown >T776 TCCTCGAGNCTGTTGGCCTACTGACACAAGGTGCTGAGACCCTTTTTACTGAGGAGGCTGAAGAAAGAGG TTGAGTCTCAGCTTCCGGAAAAGGTTGAGTATGTGATCAAGTGTGACATGTCAGCTCTGCAGAAGATTCT GTACCGTCACATGCAAGCCAAGGGGATCCTCCTCACGGACGGGTCTGAGAAAGATAAGAAGGGGAAAGGA GGTGCCAAGACACTTATGAACACCATCATGCAGCTGAGAAAAATATGCAACCACCCATATATGTTTCAGC ACATTGAGGAATCCTTTGCTGAACACCTGGGCTATTCGAATGGGGTCATCAATGGGGCTGAGCTGTATCG GGCCTCGGGAAAGTTTGAGCTGCTTGATCGTATTCTGCCCAAATTGAGAGCGACTAACCACCGCGTGCTG CTTTTCTGCCAGATGACGTCACTCATGACCATTATGGAGGATTACTTTGCTTTTCGGAACTTCCTGTACC TGCGCCTTGACGGCACCACCAAGTCTGAAGATCGTGCTGCTTTGCTAAAGAAATTCAATGAAC
Notes:	The probe template was PCR amplified from IMAGE:1907823 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1907823 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000706 same experiment
	EMAGE:29208 same embryo
	EMAGE:31689 same embryo
	EMAGE:29336 same embryo
	EMAGE:30554 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source