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EMAGE:292

Wnt7a wingless-related MMTV integration site 7A ( MGI:98961)
TS17
in situ hybridisation

Data Images
EMAGE:292
Figure 2E of Loomis et al., 1998 [PMID:9463360] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
Image annotations: d - dorsal; v - ventral. This limb was staged according to Wanek et al., 1989 [PMID:2926360] as stage 3/4.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
limb
detected detected
regional
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:9297
Entity Detected:Wnt7a, wingless-related MMTV integration site 7A ( MGI:98961)
Notes:The probe used in this study by Loomis et al., 1998 [PMID:9463360] is indicated as being that used by Parr & McMahon, 1995 [PMID:7885472] which was previously described by Parr et al. 1993, [PMID:8275860] as a "400bp SmaI-EcoRI (coding and 3' untranslated)" fragment.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Loomis et al., 1998 [PMID:9463360] Indexed by GXD.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9463360] Loomis CA, Kimmel RA, Tong CX, Michaud J, Joyner AL 1998 Analysis of the genetic pathway leading to formation of ectopic apical ectodermal ridges in mouse Engrailed-1 mutant limbs. Development (125):1137-48
 [ doi:10.1038/374350a0] [ PMID:7885472] Parr BA, McMahon AP 1995 Dorsalizing signal Wnt-7a required for normal polarity of D-V and A-P axes of mouse limb. Nature (374):350-3
 [ PMID:8275860] Parr BA, Shea MJ, Vassileva G, McMahon AP 1993 Mouse Wnt genes exhibit discrete domains of expression in the early embryonic CNS and limb buds. Development (119):247-61
 [ doi:10.1002/jez.1402490109] [ PMID:2926360] Wanek N, Muneoka K, Holler-Dinsmore G, Burton R, Bryant SV 1989 A staging system for mouse limb development. J Exp Zool (249):41-9
Links:MGI:1339621 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI