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EMAGE:29202

Sfrs1 ( MGI:98283)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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euxassay_000704_13 euxassay_000704_01 euxassay_000704_02 euxassay_000704_03 euxassay_000704_04
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euxassay_000704_05 euxassay_000704_06 euxassay_000704_07 euxassay_000704_08 euxassay_000704_09
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euxassay_000704_10 euxassay_000704_11 euxassay_000704_12 euxassay_000704_14 euxassay_000704_15
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euxassay_000704_16 euxassay_000704_17 euxassay_000704_18 euxassay_000704_19 euxassay_000704_20
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euxassay_000704_21 euxassay_000704_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3784
Entity Detected:Sfrs1, ( MGI:98283)
Sequence:sense strand is shown

>T3784
TGGCCTCGAGGCCAGATTCGGACGAGGGTTACCAATCATCTTATGTACGAGAGCGAGATCTGCTATGACG
GGGAGAATAGCGTGGTGATCCTCTGCTTCTCCTTGGGGAGTAACTGCGACTCCTGCTGTTGCTTCTGCTA
CGGCTTCTGCTACGACTACGGCTTCGAGATCGAGATCTTCCATAACTTGGACTTCTGGGCCCATCAACTT
TAACCCGGATGTAGGCAGTTTCTCCCTCGTGAGATCTAAACTTAGTGTTATCCAGTTTTCGAACTGCATA
CGTCATATCTTCTTTCCGTACAAACTCCACGACACCAGTGCCATCTCGGTAAACATCAGCGTAACATACA
TCACCTGCCTCACGCATGTGATCCTTTAAGTCCTGCCAGCTTCCACTCGGAGGCAGTCCAGAGACAACCA
CTCTGTTCTCGGACCGCCTGGACGGCGGGCCATAGCGGCCTCTCGGGGCGCCGCCGCCTCCACCCCCGCC
GCCGCCTCGGCCGGTCCC
Notes:The probe template was PCR amplified from IMAGE:391034 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:391034 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000704 same experiment
 EMAGE:31713 same embryo
 EMAGE:30052 same embryo
 EMAGE:29720 same embryo
 EMAGE:29215 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS