EMAGE mouse gene expression spatial database

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EMAGE:29263

Itgb2 integrin beta 2 ( MGI:96611)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_019756_01	euxassay_019756_02	euxassay_019756_03	euxassay_019756_04	euxassay_019756_05

euxassay_019756_06	euxassay_019756_07	euxassay_019756_08	euxassay_019756_09	euxassay_019756_10

euxassay_019756_11	euxassay_019756_12	euxassay_019756_13	euxassay_019756_14	euxassay_019756_15

euxassay_019756_16	euxassay_019756_17	euxassay_019756_18	euxassay_019756_19	euxassay_019756_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T56086
Entity Detected:	Itgb2, integrin beta 2 ( MGI:96611)
Sequence:	sense strand is shown >T56086 CAACCAGTTTCAGACAGAGGTCGGCAAGCAACTGATTTCCGGAAACCTGGACGCCCCTGAGGGTGGGCTG GATGCCATAATGCAAGTTGCTGCATGTCCGGAGGAAATTGGCTGGCGCAATGTCACGAGGCTGCTGGTGT TTGCCACAGACGATGGCTTCCACTTTGCTGGTGATGGCAAACTGGGTGCCATCCTGACCCCCAATGATGG CCGCTGCCACCTGGAGGATAACATGTACAAGAGGAGCAATGAGTTCGACTACCCATCCGTGGGTCAGCTG GCACACAAACTTTCCGAGAGCAACATCCAGCCCATCTTTGCGGTGACAAAGAAGATGGTGAAAACGTATG AGAAACTCACGGAGATCATCCCCAAGTCAGCAGTGGGGGAACTGTCTGACGACTCCAGCAACGTGGTGCA GCTCATCAAGAATGCCTACTATAAACTCTCCTCTAGAGTCTTCCTGGACCACAGCACCCTCCCGGACACC CTGAAAGTCACCTATGACTCCTTCTGCAGTAATGGAGCATCGAGTATAGGCAAATCCCGTGGGGACTGTG ATGGCGTACAGATCAACAACCCGGTCACCTTCCAGGTAAAGGTCATGGCTTCCGAGTGTATCCAGGAGCA GTCCTTTGTCATCCGGGCACTGGGTTTCACGGATACAGTGACCGTGCAGGTCCGTCCCCAGTGTGAGTGT CACTGCCGGGACCAGAGTCGGGAGCAGAGTCTCTGTGGAGGCAAGGGAGTCATGGAGTGTGGTATCTGCA GGTGTGAGTCTGGCTACATTGGGAAAAACTGTGAGTGCCAGACTCAGGGTCGGAGCAGCCAGGAGCTGGA GAGAAACTGTCGGAAGGACAATAGTTCCATCGTGTGCTCAGGGCTTGGGGACTGCATCTGTGGGCAGTGT GTATGCCATACCAGTGACGTCCCCAACAAAGAGATCTTTGGGCAATACTGCGAGTGTGACAATGTCAACT GTGAGAGATATAACAGCCAAGTCTGCGGTGGCTCAGATCGGGGTTCCTGCAACTGTGGCAAATGTAGTTG CAAGCCCGGTTACGAGGGCTCGGCCTGCCAGTGTCAGAGGTCCACCACGGGCTGTCTGAATGCACGGCTG GTAGAGTGCAGTGGCCGTGGCCACTGCCAATGCAACAGGTGCATATGTGACGAAGGCTACCAGCCACCGA TGTGTGAGGATTGTCCCAGCTGTGGCTCGCACTGCAGGGACAACCACACCTCTTGTGCCGAGTGCCTGAA GTTTGATAAGGGCCCTTTTGAGAAGAACTGTAGTGTTCAGTGTGCTGGTATGACGCTGCAGACTATCCCT TTGAAGAAAAAGCCCTGCAAGGAGAAGGACTCGGAAGGCTGTTGGATAACTTACACTTTGCAGCAGAAGG ACGGAAGGAACATTTACAACATCCATGTGGAGGACAGTCTAGAGTGTGTGAAGGGCCCCAATGTGGCTGC CATCGTAGGGGGCACCGTGGTAGGTGTCGTACTGATTGGTGTCCTCCTCCTGGTCATCTGGAAGGCCCTG ACCCACCTGACTGACCTCAGGGAGTACAGGCGCTTTGAGAAGGAGAAACTCAAGTCCCAATGGAACAATG ACAACCCCCTCTTCAAGAGTGCTACGACAACGGTCATGAACCCCAAGTTTGCTGAAAGCTAGAGCATGAG TTATCATAATCAAGCAGATGTGACCCCCTCAGACCACGCCTCCTCCCCTCTGCAAACACAACGTGGCTTA CAGCTCACCCCAGTGCTGCCAAGGATCCAAAAGCCTGCTCGGTTTCTTCCGCCATTATATCAAG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. .
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_019756 same experiment
	EMAGE:21709 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source