EMAGE mouse gene expression spatial database

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EMAGE:29456

Tardbp TAR DNA binding protein ( MGI:2387629)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_002419_11	euxassay_002419_12	euxassay_002419_13	euxassay_002419_14	euxassay_002419_15

euxassay_002419_16	euxassay_002419_17	euxassay_002419_18	euxassay_002419_19	euxassay_002419_20

euxassay_002419_21	euxassay_002419_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5064
Entity Detected:	Tardbp, TAR DNA binding protein ( MGI:2387629)
Sequence:	sense strand is shown >T5064 CCACGCGTCCGCGGAGCGGTAGCGCGGCTGTTGTCGGATTCCTTCCCGTCTCTGCTTCCTCCTTGTGCTT CCTAGCAGTGGCCTAGCGGATTTAAGCAAAGATGTCTGAATATATTCGGGTAACAGAAGATGAGAACGAT GAACCCATTGAAATACCATCAGAAGACGATGGGACGGTGTTGCTGTCCACAGTTACAGCCCAGTTTCCAG GGGCATGCGGCCTGCGCTACCGGAATCCCGTGTCTCAGTGTATGAGAGGAGTCCGACTGGTGGAAGGAAT TCTGCATGCCCCAGATGCTGGCTGGGGCAATCTGGTATATGTTGTCAACTATCCCAAAGATAACAAAAGG AAAATGGATGAGACAGATGCTTCCTCTGCAGTGAAAGTGAAAAGAGCAGTCCAGAAAACATCTGACCTCA TAGTGTTGGGTCTCCCCTGGAAAACAACTGAGCAGGATCTGAAAGACTATTTCAGTACTTTTGGAGAGGT TCTTATGGTTCAGGTCAAGAAAGATCTTAAAACTGGTCACTCGAAAGGGTTTGGCTTTGTTCGATTTACA GAATATGAAACCCAAGTGAAAGTAATGTCACAACGACATATGATAGATGGGCGATGGTGTGACTGTAAAC TTCCCAACTCTAAGCAAAGCCCAGACGAGCCTTTGAGAAGCAGAAAGGTGTTTGTTGGACGTTGTACAGA GGACATGACTGCTGAAGAGCTTCAGCAGTTTTTCTGTCAGTATGGAGAAGTGGTAGATGTCTTCATTCCC AAACCATTCAGAGCTTTTGCCTTCGTCACCTTTGCAGATGATAAGGTTGCCCAGTCTCTTTGTGGAGAGG ATTTGATCATTAAAGGAATCAGCGTGCATATATCCAATGCTGAACCTAAGCATAATAGCAATAGACAGTT AGAAAGAAGTGGAAGATTTGGTGGTAATCCAGGTGGCTTTGGGAATCAGGGTGGGTTTGGTAACAGTAGA GGGGGTGGAGCTGGCTTGGGAAATAACCAGGGTGGTAATATGGGTGGAGGGATGAACTTTGGTGCTTTTA GCATTAACCCAGCGATGATGGCTGCGGCTCAGGCAGCGTTGCAGAGCAGTTGGGGTATGATGGGCATGTT AGCCAGCCAGCAGAACCAGTCGGGCCCATCTGGGAATAACCAAAGCCAGGGCAGCATGCAGAGGGAACCA AATCAGGCTTTTGGTTCTGGAAATAATTCCTACAGTGGTTCTAATTCTGGTGCCCCCCTTGGTTGGGGGT CAGCATCAAATGCAGGATCGGGCAGTGGTTTTAATGGGGGCTTTGGCTCGAGCATGGATTCTAAGTCTTC TGGCTGGGGAATGTAGGTGGTGGGGGGTGGTTAGTAGGTTGGTTATTAGGTTAGGTAGATTTAGAATGGT GGGATTCAAATTTTTCTAAACTCATGGTAAGTATATTGTAAAATACATATGTACTAAAATTTTCAGATTG GTTTGTTCAGTGTGGAGTATATTCAGCAGTATTTTTGACATTTTTCTTTAGAAAAAAAGAGGGGAAAGCT AAATGAATTTTATAAGTTTTGTTATATAAAGGGTTAAAATACTGAGTGGGTGAAAGTGAACTGCTGTTTG CCTAATTGGTAAACCAACACTACAATTGATCTCAGAAGGTTTCTCTGTAATATTCTATCATTGAAATTGT TAATGAATTCTTTGCATGTTCAGAGTAGAAACCATTGGTTAGAACTACATTCTTTTCTCCTTATTTTAAT TTGAATCCCACCCTATGAATTTTTTCCTTAGGAAAATCTCCATTTGGGAGATCATGATGTCATGGTGTTT GATTCTTTTGGTTTTGTTTTTAACACTTGTCTTCCTTCATATACGAAAGTACAATATGAAGCCTTCATTT AATCTCTGCAGTTCATCTCATTTCAAATGTTTATGGAAGAAGCACTTCATTGAAAGTAGTGCTGTAAATA TTCTGCCATAGGAATACTTCTGTCTACATGCTTTCTCATCCAAGAATTCGTCATCACGCTGCACAGGCTG CGTCTTTGACGGTGGGTGTTCCATTTTTATCCGCTACTCTTTATTTCATGGAGTCGTATCAACGCTATGA ACGCAAGGCTGTGATATGGAACCAGAAGGCTGTTTGAACTTTTGAAACCTTGTGTGGGATTGATGGTGGT GCCGAGGCATGAAAGGCTAGTATGAGCGAGAAAAGGAGAGCGCGTGCAGAGACTTGGTGGTGGAAAATGG ATATTTTTTAACTTGGAGAGATGTGTCACTCAATCCTGTGGCTTTGGTGAGAGAGTGTGCAGAGAGCAAT GATAGCAAATAACGTACGAATGTTTTACATCAAAGGACATCCACATCAGTTGGAAGACTTTGAGTCTTGT TCTTAGGAAACCCACTTTAGTTGAATGTGTTAAGTGAAATACTTGTACTTCCCTCCCCCTCTGTCAACTG CTGTGAATGCTGTATGGTGTGTGTTCTCCTCTGTTACTGATCTGGAAGTGTGGGAACGTGAACTGAAGCT GATGGGCTGCGAACATGGACTGAGCTTGTGGTGTGCTTTGCAGGAGAACTTGGAAGCAGAGTTCACCAGT GAGCTCAGGTGTCTCAAAGAAGGGTGGAAGTTCTCATGTCTGTTAGCTATTCATAAGAATGCTGTTTGCT GCAGTTCTGTGTCCTGTGCTTGGATGCTTTTTTATAAGAGTTGTCATTGTTGGAAATTCTTAAATAAAAC TGATTTAAATAATATGTGTCTTTGTTTTGCAGCCCTGAATGCAAAGAATTCATAGCAGTTAATTCCCCTT TTTGACCCTTTTGAGATGGAACTTTCATAAAGTTTCTTGGCAGTAGTTTATTTTGCTTCAAATAAACTTA TTTGAAAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5321007 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5321007 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002419 same experiment
	EMAGE:32052 same embryo
	EMAGE:29841 same embryo
	EMAGE:29455 same embryo
	EMAGE:29842 same embryo
	EMAGE:29812 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source