EMAGE mouse gene expression spatial database

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EMAGE:29472

Dusp10 dual specificity phosphatase 10 ( MGI:1927070)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_004246_11	euxassay_004246_12	euxassay_004246_13	euxassay_004246_14	euxassay_004246_15

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euxassay_004246_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6129
Entity Detected:	Dusp10, dual specificity phosphatase 10 ( MGI:1927070)
Sequence:	sense strand is shown >T6129 GCAAAAGAGAAAGTAACTCTGTGTTGCTGCTGGGGATCAATGAAGCTGAGTGAATGGGGGCGCACTGCGC GAGCGCATAGCTGGAGCGGGCGCCAGATGGTAGAGGGCTACGCTTATTGATGAAACTCTTGACTTCTTCT TGAATTGCCAGTTTTCAGCCTCCTCATGCCTCCATCTCCTTTAGACGACAGGGTAGTAGTGGCACTATCT AGGCCTGTCCGACCTCAGGATCTCAACCTTTGTTTAGACTCAAGCTACCTTGGCTCTGCCAGCCCAGGTA GTGGTAGCCACGCTCCTGTCCTTGCCACCGCCGTTGTGACCCTCAAGGCTGCGAATCTGACGTATATGCC CTCATCCAGCGGCTCTGCCCGCTCCCTGAATTGCGGATGCAGCAGCACTAGCTGCTGCACTGTGGCAACC TACGACAAGGATCATCAGGCCCAGACTCAAGCCATTGCTGCAGGCACTGCCACCACCGCCATAGGAACCT CGACCACCTGCCCTGCTAACCAGATGGTCAACAACAATGAAAACACAGGCTCTGTGTTAAGTCCATCGGG TGGGGTGGGCAGCCCTGTGTCAGGGACTCCCAAGCAGCTAGCCAGCATCAAGATCATCTACCCCAATGAC CTGGCAAAGAAGATGACCAAATGCAGCAAGAGTCACCTGCCCAGCCAGGGTCCCGTCATCATCGATTGCA GGCCTTTCATGGAGTACAACAAGAGTCACATCCAAGGAGCTGTCCACATTAACTGTGCCGATAAGATCAG CCGGCGAAGGCTGCAGCAGGGCAAGATCACTGTCTTAGACTTGATTTCCTGTAGGGAAGGCAAAGACTCT TTCAAGAGGATCTTTTCCAAAGAAATTATAGTTTATGATGAGAATACCAATGAGCCAAGCCGTGTGACAC CATCCCAGCCACTTCACATAGTCCTTGAGTCCCTGAAGAGAGAAGGCAAAGAACCCCTGGTATTGAAAGG GGGACTCAGTAGTTTTAAACAGAACCATGGAAACCTCTGTGACAACTCCCTCCAGCTCCAAGAGTGCCGG GAGGTGGGGGGTGGTGCATCTGCGGCCTCGAGCATGCTACCTCAGTCTGTCCCCACCACCCCTGACATCG AGAACGCAGAGCTAACGCCCATCCTGCCCTTCCTGTTCCTCGGCAATGAGCAGGATGCTCAGGACCTAGA CACCATGCAGAGGCTCAACATCGGCTATGTCATCAACGTCACCACGCACCTTCCTCTGTACCATTATGAG AAAGGCCTCTTCAACTACAAGAGGCTGCCAGCCACAGACAGCAACAAACAGAACCTGCGGCAGTACTTTG AAGAGGCCTTCGAGTTCATCGAGGAAGCTCACCAGTGTGGGAAGGGCCTTCTCATCCACTGCCAGGCCGG CGTGTCCCGATCCGCCACCATCGTCATCGCCTACTTGATGAAGCACACACGGATGACCATGACTGACGCT TACAAATTCGTCAAAGGCAAACGACCAATTATTTCCCCGAACCTCAACTTCATGGGGCAGTTGCTGGAAT TTGAGGAAGACCTAAACAACGGCGTGACGCCAAGAATCCTTACACCAAAGCTCATGGGCATGGAGACAGT TGTGTGACAACGGGCAGGACGGAAAGGGCTGTGCTCTCTCAGGAGACGAAAAGGAGGGAAGGTGGATTCT AGCTTGTCGCTCTTCTTTCCTTTTCTTTTCTCTTCTTTTCTTTTCTTTTTCTTCTTTTCTTTCTCTTTCT TTCTCTCTTTTTTTTAAGGTTGGGAGTGTTTGTGAAAGTAAACAAACTTGTCTAAAGACTCTATTTTTAA TAAGTGTAAGAAGACTGTAACTTTTGATGCGAATGAGACCCACTTCTCTTAAACTGGTATTGCAGTTAGG TTAAAGAGGTCTTTTTTGTTTTGTTTTGTTTTTTTAAGCCAACAAATAAAAATATTATAAAACTTGTTTC TTCCCAGTTTCCCTTGTAAGCTTATTGTAGAGTTTTGAGTAAATAGTCTGTGCGGGCTCATAGACCGAAG ATACCACCCTTCAGTTACAAAGAACCAAAAGGAAATGGAAAAGACTCTAGACCTGGAACTGACTGCACAA CCATGTCAAAAGCAAACCCTGCCAAACCGTGCCCAGTTGTGCTCACTGGTGTGAAGAAAGGGCCGGCAAG CTCAGTGGACTCGGCTCGCCTCACACCTTCTCTGGGGAGACAGGCTAGTGTCGGTTTTGTGTGTTCTATG CTCCGGATATCTTTTCCCCTTCTGTGGGTCTGAGAGAACTTTTTGTTTGTTTGTTTGTTTGTTTGGCAGA AGGAAACTGACCATTCTACCATGTGCCTTTGCTGGCACTCGTGCAATAAAAATGGTGTTTGGAGTGTGCA GCTGAGGGAGAGCTGGCAGCTGAGTGAGGAGTGGCGTTGGAGACAGGCATTCAACAGTACCAGTGGCTTC CCCTCCCTCCTTTCTCCTCCCCCCCCTTTCTTCCTCATCCCCTTTTATTTGTCTTTCTTTTGATTTGATT CTGGTTACAGTGCCATACACCTTGTTACATATGTATATCAGAATGTAAAAAAACAAAAAAACAAAAAAAA ACAAAAATTTATTTAAAAAAATATTTTTCGCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3495358 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3495358 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004246 same experiment
	EMAGE:29470 same embryo
	EMAGE:29473 same embryo
	EMAGE:29487 same embryo
	EMAGE:29269 same embryo
	EMAGE:31358 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source