EMAGE mouse gene expression spatial database

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EMAGE:29541

Sphk2 sphingosine kinase 2 ( MGI:1861380)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_002008_06	euxassay_002008_07	euxassay_002008_08	euxassay_002008_09	euxassay_002008_10

euxassay_002008_11	euxassay_002008_12	euxassay_002008_13	euxassay_002008_14	euxassay_002008_15

euxassay_002008_16	euxassay_002008_17	euxassay_002008_18	euxassay_002008_19	euxassay_002008_20

euxassay_002008_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4588
Entity Detected:	Sphk2, sphingosine kinase 2 ( MGI:1861380)
Sequence:	sense strand is shown >T4588 CAAGAAGGGGCTCAGTCCTGGCTCACCCTCTTCCTGGAGGAAACTCGGCCAGACACCCAGAGGCTGGTCC AGGAGCTGACCACAAGCCAAGAGCCTAGGAGTGCTTGGGACTGAACCAGGGTCATGGCCCCACCACCACT ACTGCCAGTGGCTGCCAGCACTCCAATCCTGCACGGCGAGTTTGGTTCCTACCCGGCCAACGGCCCACGG TTTGCCCTCACCCTCACAACACAAGCCCTACACATACAGCGACTACGCCCAAAGCCAGAAGCCCGGCCCC GAGATGGTCTAGTCTCTCTGGATGAGGTCTCGGGCTGTGGCACCCTGCAGAGCCGTAGCCCCGAGGACAC TGCAGCCTACTTCTGCATCTACACCTACCCACGTGGCCGTCGAGGGGGCCGGCGCAGAGCTACGCGGACC TTCCGGGCGGATGGGGCCACCACTTATGAGGAGAATCGTGCAGAGGCCCAGCGCTGGGCCACTGCCCTCA CGTGTCTCCTCCGAGGAGTGCCTCTGTCAGGGGACCAGGAAATCACCCCTGAATTGCTGCCCCGGAAGCC CAGGCTGCTCATATTGGTCAATCCCTTTGGGGGGCGGGGCCTGGCCTGGCAGCGCTGTATGGACCACGTG GTGCCAATGATCTCTGAAGCTGGGCTGTCCTTCAACCTCATACAGACAGAACGACAGAACCATGCCCGTG AGCTGGTGCAGGGGTTAAGCCTGAGTGAGTGGGAAGGCATTGTCACTGTGTCTGGAGACGGGCTGCTTTA CGAGGTGCTGAATGGGCTCCTTGATCGGCCAGACTGGGAGGATGCCGTGCGGATGCCCATTGGTGTCCTC CCCTGTGGATCGGGCAATGCGCTAGCTGGGGCGGTGAACCATCATGGCGGGTTTGAGCAGGTTGTCGGTG TTGACCTGTTGCTCAACTGCTCGCTTCTTCTCTGCCGTGGTGGCAGCCATCCTCTGGACCTGCTCTCTGT GACGCTAGCCTCGGGATCCCGCTGTTTTTCCTTCCTGTCAGTGGCCTGGGGATTCTTGTCAGATGTGGAC ATTCACAGTGAGCGCTTCAGGGCCCTGGGCAGCGCTCGATTCACACTGGGTGCAGTGCTAGGCCTGGCCT CGTTGCATACCTACCGTGGACGCCTCTCCTACCTCCCCGCTACCACAGAACCAGCCTTGCCCATCCCAGG CCACAGTCTGCCTCGAGCCAAGTCAGAACTAGTCTTGGCTCCAGCCCCAGCCCCCGCCGCCACCCACTCG CCTCTACATCGATCTGTGTCTGACCTGCCCCTGCCCCTTCCCCAGCCTGCCTTGGTCTCCCCTGGCTCCC CTGAGCCCCTGCCTGACCTGTCCCTCAATGGTGGTGGTCCAGAGCTGACTGGAGACTGGGGAGGAGCTGG GGATGCACCTCTGTCCCCAGACCCACTGCTGCCTTCATCCCCCAACGCTCTCAAAACAGCTCAGCTTTCA CCCATCGCTGAAGGGCCCCCAGAAATGCCAGCATCTTCGGGGTTCCTGCCTCCCACCCACAGTGCCCCAG AAGCCTCTACCTGGGGCCCAGTGGACCACCTCCTCCCTCCCCTGGGCTCTCCACTGCCCCAAGACTGGGT GACAATAGAGGGGGAGTTTGTACTCATGTTGGGCATCTTGCCGAGCCACCTCTGCGCAGACCTGATGGCA GCCCCACATGCACGCTTTGATGATGGCGTTGTGCACCTGTGTTGGGTGCGGAGCGGCATCTCACGGGCTG CACTTCTACGCATTTTGCTGGCCATGGAGCATGGAAACCACTTCAGCCTGGGCTGCCCCCATCTGGGCTA TGCTGCAGCACGTGCCTTCCGCCTTGAACCACTCACGCCTCGTGGCCTGCTCACTGTAGATGGGGAGTTA GTGGAGTATGGGCCAATACAGGCGCAGGTGCACCCAGGTCTCGCCACGCTGCTCACTGGGCCTGCAGGTC AAAAGCCACAAGCCTGAACGAGCCTAAAAGCATGGCGAGTTGGTGGAACCAGCGCCCCATAGGCTAAGAT CTATCATTTACAGGTAGAAGTGGGGCCCGCACTCAGAACTGTGAGGAGGGTGGAGAGTGGTCCTGACCCT CAGTTCCCAGAGGACCTAGAGGCTCGAGGGTGGGGCCTGCCTTTCTTGATGTCCAATGATGGGGCCTGGA ATGTATGAGCTAGCAAGGCTTCTTCAGCTTATTGACCAGCCAGGGTTTCTTCTTGCCTACTCCGGTGCCT CTACTTGACTGGCCAATCAGCCCTTGAGGGGCAGGTTCCCCCAGGTGGTCCCCAGATTTGCACTAATGTT CCTCCCCTGGCCAGTTAGGGATGGGATGTTCTGTGTCTTGTGTGTCCCTCTCCCTAGTCTAAAAAGCAAT TGAAAAGGTCTATGCAATAAAGGTTGTTGCTTCCCTCTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4987298 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4987298 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002008 same experiment
	EMAGE:29538 same embryo
	EMAGE:31868 same embryo
	EMAGE:30546 same embryo
	EMAGE:30545 same embryo
	EMAGE:31458 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source