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EMAGE:29551

Btbd4 (Btbd4)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:29551 EMAGE:29551 EMAGE:29551 EMAGE:29551 EMAGE:29551
euxassay_002483_01 euxassay_002483_02 euxassay_002483_03 euxassay_002483_04 euxassay_002483_05
EMAGE:29551 EMAGE:29551 EMAGE:29551 EMAGE:29551 EMAGE:29551
euxassay_002483_06 euxassay_002483_07 euxassay_002483_08 euxassay_002483_09 euxassay_002483_10
EMAGE:29551 EMAGE:29551 EMAGE:29551 EMAGE:29551 EMAGE:29551
euxassay_002483_11 euxassay_002483_12 euxassay_002483_13 euxassay_002483_14 euxassay_002483_15
EMAGE:29551 EMAGE:29551 EMAGE:29551 EMAGE:29551 EMAGE:29551
euxassay_002483_16 euxassay_002483_17 euxassay_002483_18 euxassay_002483_19 euxassay_002483_20
EMAGE:29551 EMAGE:29551 EMAGE:29551
euxassay_002483_21 euxassay_002483_22 euxassay_002483_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5111
Entity Detected:Btbd4, (Btbd4)
Sequence:sense strand is shown

>T5111
GACGGACGAACGGACTGGCGGGCGGACCAAGCCACCCGAGCGAGCGCGCAGCTCGGACCCTCCCGGACGC
AGCTCTCGCCAGTGGCCGCGCGATCACCGGTCCGCGGCCACGGCGCCAGCCCGCGCCCGCGCCCCATGGG
GTCACAGAGTCTATAGAAGACACAGCAGCAAGACTCCCAGATGAACAACCGAAAGGAAGATATGGAAATC
ACTTCTCACTACCGGCATCTGCTTCGAGAGCTCAATGAGCAGAGGCAGCACGGAGTCCTCTGTGATGCGT
GCGTCGTGGTGGAGGGCAAGGTCTTCAAGGCACATAAGAACGTCTTGCTTGGGAGCAGCCGCTACTTTAA
GACGCTCTACTGCCAGGTACAGAAGACATCTGACCAGGCCACCGTCACTCACTTGGACATTGTTACAGCC
CAGGGCTTCAAGGCCATTATTGACTTCATGTACTCCGCCCATCTGGCTCTCACTAGTAGGAATGTCATCG
AGGTGATGTCAGCTGCCAGCTTCCTACAGATGACTGACATTGTGCAGGCCTGCCATGATTTCATCAAGGC
TGCACTGGACATCAGCATAAAGTCAGATGCCTCCGATGAACTCTCAGAATTTGAGATTGGCACCCCAGCC
AGCAACAGTACAGAGGCGTTGATCTCAGCTGTGATGGCTGGAAGGAGTATCTCCCCATGGTTGGCTCGGA
GAACAAGTCCTGCCAATTCTTCTGGAGACTCTGCCATTGCCAGCTGTCATGAAGGAGGAAGCAGCTATGG
GAAGGAGGACCAGGAACCCAAAGCTGATGGCCCTGATGACGTTTCTTCACAGTCTTTGTGGCCTGGAGAT
GTAGGCTATGGGTCTCTGCGCATCAAGGAAGAACAGATTTCACCATCACATTATGGAGGGAGTGAGCTTC
CATCTTCCAAGGACACTGCAATACAGAATTCTTTATCAGAACAGGGTTCTGGGGATGGCTGGCAGCCCAC
AGGCCGGAGGAAGAATCGGAAAAACAAAGAGACTGTCCGACACATCACCCAGCAGGTGGAGGAGGACAGC
CAGGCTGGCTCTCCAGTACCTTCATTCCTACCCACATCGGGATGGCCTTTCAGCAGCCGAGACTCAAATG
TAGACCTGACGGTCACTGAGGCCAGCAGCTTGGACAGCCGAGGCGAGAGAGCAGAGCTCTATGCTCACAT
CGATGAGGGCCTACTAGGAGGAGAAACCAGCTACTTGGGCCCACCCCTCACCCCAGAGAAGGAAGAAGCA
CTACACCAGGCTACTGCAGTGGCCAATCTTCGTGCTGCACTCATGAGTAAGAACAGTCTGCTGTCACTCA
AGGCTGACGTGCTCGGTGATGATGGCTCACTTCTGTTCGAGTACCTGCCCAAAGGTGCCCACTCACTGTC
TCGTAAGTGCAAGTTCTGGTGTGTCACTGTGTCTTCCTTTGGTTTAAGCACCTCAGTTCAGCCCTTCAGA
CCCTGGAGTCACTGAGGATGAGGCTTGTAGCTAACCTGTCTGTCAGGCAGATTGCTGCGGGTTGCAGCCA
CCTTCCAACTCAAGGTCTTTCAGAAAGTAGATTGGCTTTGAGTCTTCTGGGACCAGCATACTTGCTTAAC
AGAACAAGAAGGGTGTTAGTATGTGTTACTGGGCAGCGAGAGGGATCAGTGAGCATTTGTTGGAATGTTT
TGTGCTCAAATTTGAAGCATCTAAACCTAAAATCCACCAAGCTTACAAATTCACACAATTAGTAAACTTA
AAACCACAAGTTCCTTTTGAGCCAGTGGCAGGTCTGGTGCGAGATAGTCCTTTGTGTCCAAGGGACAAGC
ACTGGCTCCCTCTACTGGTCCCTGTGCCCAGCCTTTGTGCTGGGATTTGTTTTGTGTGGGAGACATGCAG
AGAGCCACTGACCCAGGGGTGTGGACCTTGCTTTGTTCTTGCTAGAAAGTGCTGGTCACATGTGCGTTTT
TAAAAAGGAGTTGAATGCTACATTTAAGACCTTAGTGTGTCTAGCTGAAATACAGACCACAGACTGACTA
TGCTGCAGCTGAAGAGCAGCTGCTATTTAGCAGCTGGCTCCCCCCAGTTGCCTTTTCCTGTGAGACAGGA
GGCATTAGCATCTGCAGAGCTCATAACCATAAGACAGGCTGCTCCCCTTGTCACAGTACATGCTGACTAT
AGCAGCCAATTATGTGATGCTGGGAGAGTAGTATGACTACCCTGGGTACCGATCTAGCCCCAGAACTTCC
TGTTTGTCTGGTACTCAGCATGAGTGAGAAAATGACTTGGTACTTTTCTCTCTGTGGGGGAGGAAGAGAC
ACACCCCACACTGGGGAACATGAGGGGTATGCTATTCCAGGAAGCCTAAGTACATATATAATGGTGACTG
GTCATCCTCCGGACCGCTGAATTACAGGACCGTTTCCAGCCACCTTTGATGCTGGTTCTTTGGAGCATGT
TTACAGTTTTGTTCCCATGTGCACTAACATATGATTGGGCTTTTGTATCGGTTTGAGGAATTATGCAGAT
TACTTATGGCAAATAAAGACCAAAGGTGGAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5326477 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5326477 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002483 same experiment
 EMAGE:29539 same embryo
 EMAGE:29282 same embryo
 EMAGE:29395 same embryo
 EMAGE:31465 same embryo
 EMAGE:29279 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS