EMAGE mouse gene expression spatial database

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EMAGE:29598

Ugt2b34 UDP glucuronosyltransferase 2 family, polypeptide B34 ( MGI:2140962)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003071_01	euxassay_003071_02	euxassay_003071_03	euxassay_003071_04	euxassay_003071_05

euxassay_003071_06	euxassay_003071_07	euxassay_003071_08	euxassay_003071_09	euxassay_003071_10

euxassay_003071_11	euxassay_003071_12	euxassay_003071_13	euxassay_003071_14	euxassay_003071_15

euxassay_003071_16	euxassay_003071_17	euxassay_003071_18	euxassay_003071_19	euxassay_003071_20

euxassay_003071_21	euxassay_003071_22	euxassay_003071_23	euxassay_003071_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4881
Entity Detected:	Ugt2b34, UDP glucuronosyltransferase 2 family, polypeptide B34 ( MGI:2140962)
Sequence:	sense strand is shown >T4881 GCCTGAAGTTAACCAAGATGCCTGTGAAAATGACAGCAGCCCTGCTCCTGCTCCTGCTCCAGCTGAGTGG CTTCTTTGGATCTGGGACTGGTGGGAAGGTGCTGGTGTGGCCAATGGAGTTCAGCCATTGGCTCAACTTG AAGACAATCCTAGACGAGCTTCTGAAGAAGGGTCATGAAGTGATGGTTCTGAGACCTTCAGCATCCTTAT CTTATGAGGTTGACAACACATCTGCTATTGAATTCGAGACTTATCCTACATCATACTCCTTATCTGAGTT AGAGGAAATTTTCTGGGAATCACTCAAGAAATATATTTATGAGCTGCCAAAGCAGTCATTTTGGGGATAC TTCTTAATGTTGCAAGAAATGGTTTGGGTAGATTCAAAATATTTTGAAAGTCTCTGTAAAGATGTAGTTT TTAACAAAGAACTCATGACAAAGCTACAAAAATCAAGGTTTGATGTGATCCTGGCAGATCCCTTCATACC CTGCGGTGATCTGCTGGCTGAGGTTCTCAAGATCCCCCTTGTGTACAGTCTCCGCTTCTTTCCTGGCTCC ACTTATGAGAAGTACAGTGGGGGACTCCCACTGCCTCCTTCCTATGTGCCTGTTGTTATGTCAGAATTGA GTGACAGGATGACTTTCATGGAGAGGGTGAGAAATGTCATCTATATGCTGTGTTTTGACTTTTGGTTCCA AACATTTAATGAGAAGAACTGGAATCAGCTTTATACTGAAGTATTAGGAAGACCCACGACACTCTCAGAG ACTATGGCCAAAGCAGACATATGGCTCATTAGGACCTACTGGGATTTAGAATTTCCTCATCCTGTCTTAC CAAATTTTGATTTTATTGGTGGCCTCCACTGCAGACCTGCCAAGCCCCTGCCTAAGGAAATAGAAGACTT TGTCCAGTCATCTGGAGAACATGGTGTTGTGGTGTTTTCCCTGGGGTCCATGGTGGGTAGCATAACAGAA GAAAGAGCCAATGTGATTGCAGCAGGCCTTGCTCAGATTCCACAGAAGGTTCTGTGGAGATTTGAGGGCA AGAAGCCAGAGACATTGGGCTCCAACACTCGTCTGTACAAATGGATCCCCCAGAATGACCTTCTAGGTCA TTCCAAAACCAGAGCGTTTATAACTCATGGTGGCACCAATGGCATCTATGAGGCGATCTACCATGGGATT CCAGTGGTTGGCATTCCTTTGTTTGGAGACCAATATGACAATATTGTTCACTTGAAGGCCAAAGGAGCAG CTGTTAGACTGGACTTTCTCACAATGTCTAGTACAGATTTGCACACTGCACTGAAGACAGTCACTAATGA CCCTTCCTATAAGGAGAATGCCATGCGGTTATCAAGAATTCACCATGATCAGCCAGTGAAGCCCCTGGAC CGAGCAGTCTTCTGGATTGAGTTTGTCATGCGTCACAAAGGAGCCAAGCATCTTCGTGTGGCAGCCCATG ACCTCAGCTGGGTCCAATACCACTCTCTGGATGTGCTTGGGTTCCTGCTGGCCTGTGTGCTGACTGTGAT GTTCATCCTCAAAAAGTGTTGCCTCTTTTGTTGCCAGAAGTTGACTAAAGCTGGAAGGAAGAAGAAAGGG GAGTAGCTGAACTGGGGAAGACTGACACATGGTCCTTTCCAGTGCATCACAGAAAGGGAGGCAATGATTT AGGGTCCTTCATCCTGTGACAAGATAGTATTTCAATAATTACTTATTAAGTCAGAAGATATTTTTTTTCT CCGAGAGATTTAATAATTCTTTTTGAAATTAGTAGTATTTCAGCCAAATATAAAATTGAAAGGGAAACAC AGTAAAATTAACACCATCAACATGAAACGGAAACAACAGACATTTTACAAATTCATTTCTGCAACATCAA AGTTAAACCAAAGAATGTTACATCTGAAGAGCTGGCTCCATGGTGCAACTATTTGTCAGGCAAGACTTCC TCCTTGATTTTTGAGGAACTCATAAAAAATCCATATCAAGTAGTGTCTATAACTCTAGTATGAGAAAACC AAGAGATGTGAAACAGACACATGAGAATTACCTGAAGCTCTCAGGCCAACTAGCCTGGTCTTCAGCAAGA AATAGCCTGCCTCATAGAATGTGGAAGGGAAACTAACACTGAAAGCTGTGCTTTGACATCTGAACATTTG TGCCATGGCATAGGTAAGACCCACTCACAAATTCATAAGCATAAGGATATAATTATACATATGTGTTCAG GCTTTATATAAACACACACACACATATAGACAACACACCACTTATAACTCATAGTTTGTTTGCGGATATA CCATGAGTAAGAAGCCCACAATTTCTACATGGTTTTGCAAGTATTGAACAAGCTTTAACTTTATTTATTG CAGACTTTACAGGATGAATATCTAGGTAACATGTTCAGGATTGGAGCAGTGTGAAACCTGTAACCCTACA TTTGTCTTTAGAACACTTGTACTTTTGCAGACTCTCATGTGGTGCAATAAGCAATAAGCAATGACCTCTT CCTTAAAGGAACTACGAATATTGGACTTGGGGCTGGGGAGATGGCTCAGCAGTTACTAACTAACTCTTCT TCTAGAGAACCCATATTCCATTCCTATCACCCATATGGTGATGATTCAGGACCAGCAGTAACTCAAGTGT CACTATGGGATCTTACACCTTCTTCTGGCCTCTCTTGGTACTGCAGAGACACATGCAAAGGAAGCCTGCA TGCACATAAAATACAATTTAGGATTAAAGACAATTTTTCATTTCATGAAATGAATTCTATTTCCATTCAA CCCTTGACAAAGAATAATGAAATTCCTTCCTTTTTATAATTAGCTTTTGGGAACAGATGTAATATTTTAA TTTATGGTTTGATAACACAGAATAAAGACTCTAAATAAGTTATTCTCACACAAGTGCAGGATGTGTGTAT GTATGTGATTCATGTCCCTTTTTATAAAACAGAATGTACTTTACCTATGCCCTCTATATTTATGCTGTGA CTTCAATATTTCTAAAAGAAATAAATGTTTTGAAATTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5098890 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5098890 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003071 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source