EMAGE mouse gene expression spatial database

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EMAGE:29599

Uroc1 urocanase domain containing 1 ( MGI:2385332)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003072_01	euxassay_003072_02	euxassay_003072_03	euxassay_003072_04	euxassay_003072_05

euxassay_003072_06	euxassay_003072_07	euxassay_003072_08	euxassay_003072_09	euxassay_003072_10

euxassay_003072_11	euxassay_003072_12	euxassay_003072_13	euxassay_003072_14	euxassay_003072_15

euxassay_003072_16	euxassay_003072_17	euxassay_003072_18	euxassay_003072_19	euxassay_003072_20

euxassay_003072_21	euxassay_003072_22	euxassay_003072_23	euxassay_003072_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4880
Entity Detected:	Uroc1, urocanase domain containing 1 ( MGI:2385332)
Sequence:	sense strand is shown >T4880 AGCTTCTGCAGACATGTCCAGCCTCCAGGAACTGTGCTCTGGTCTGCCCCTACGACCCCTTCCTGAGAAC CGGGGTCGCTGGGCTGGGGTGCCACATGCTCCGGTTAGAACTCCGAACCTCAGCCCTGAAGAAGAGCAGC TGGCCCTGAGGAATGCCTTGCGCTATTTCCCACCTGATGTCCAGAAACTGTTGGCCCTGGAGTTTGCCCA GGAGCTAAGACAGTTTGGACACATCTACATGTACCGGTTCTGCCCCAGCATTGAGATGAGAGCCTACCCA ATTGATCAGTACCCATGCCGGACACGGGCAGCTGCAGCCATCATGCACATGATCATGAACAACTTGGACC CTGCTGTGGCCCAGTTTCCACAGGAGCTGGTGACCTACGGAGGCAACGGGCAAGTGTTCAGCAACTGGGC CCAGTTCCGGCTGACCATGTCCTACCTGTCGAAGATGACTGAGGAACAGACTCTGGTCATGTACAGTGGA CACCCTCTGGGCCTCTTCCCCAGCAGCCCCCGTGCCCCACGGCTGGTCATCACTAACGGGATGGTCATTC CCAACTACTCCTCCAGGACAGAGTATGAGAAGCTGTTTGCCTTGGGGGTGACCATGTATGGCCAGATGAC AGCGGGCAGCTACTGCTACATTGGTCCCCAGGGAATCGTCCATGGCACAGTGCTCACTGTGCTGAATGCT GGTCGTCGGTACCTGGGCATCGAGAACTTGGCAGGGAAGGTCTTTGTCACCTCTGGGCTTGGCGGGATGA GTGGGGCTCAAGCCAAGGCAGCAGCCATCGTTGGATGCATTGGGGTGATTGCGGAGGTGGATAAAGCTGC ACTTGTAAAACGCCACCGGCAAGGCTGGCTGATGGAGGTGACAGACAGCTTGGACCACTGCATTGCAAGA CTCAGGGAAGCAAGGAAAAAGAAGGAGGTTCTCAGCCTCGGCTACCATGGCAACGTGGTGGATCTTTGGG AGCGCCTTGTCCGTGAACTGGACACAACGGGGGAACTCTTGGTGGACCTCGGGTCAGACCAGACATCCTG TCACAACCCGTTCAATGGCGGCTACTACCCTGTGCAGCTCAGCTTCTCAGAGGCCCAGAGCCTCATGTCC TCCAACCCTGCTGCCTTCAAGCACCTGGTGCAGGAAAGCCTGAGGAGGCATGTCGCAGCCATCAACAGGT TGGCCCAAGAGAAGTTCTTCTTCTGGGACTATGGTAATGCCTTCCTCTTGGAGGCTCAGAGAGCAGGAGC AGATGTAGAGAAGAAAGGAGCCAACAAGATGGAGTTCCGCTACCCCTCCTATGTCCAGCACATTATGGGG GATATATTCTCCCAGGGCTTTGGGCCCTTCCGCTGGGTATGCACATCAGGGGACCCCCAGGACCTGGCTG TCACTGATCATCTGGCCACATCTGTACTGGAGAAAGCCATTGCTGATGGAGTGAAGGCATCTGTGAAGCT ACAGTATATGGATAATATACGCTGGATCCGAGAGGCTGCCAAGCATCAGCTGGTGGTGGGCTCCCAGGCA AGGATCCTGTACTCAGACCAGAAAGGCCGTGTGGCCATTGCTGTGGCCATTAACCAGGCCATCGCCAGTG GGAAGATCAAGGCACCAGTGGTCCTGAGCCGTGACCACCATGATGTGAGTGGCACTGACAGCCCCTTCAG GGAGACTTCGAATATTTATGACGGTTCTGCCTTCTGCGCAGACATGGCCGTGCAGAACTTCGTGGGAGAT GCCTGTCGTGGTGCCACCTGGGTTGCACTTCACAATGGAGGGGGTGTAGGCTGGGGTGAAGTCATCAATG GGGGATTTGGCCTTGTTCTAGATGGAACTGCAGAGGCTGAGCAGAAAGCCAGGATGATGCTCAGCTGGGA TGTCTCCAACGGTGTGGCACGACGCTGCTGGTCTGGAAACCCCAAGGCCTATGAGATCATCTGCCAGACA ATGCAGGAGAACAGTGGCCTGGTGGTGACGCTTCCCCATGAGGTGGCAGATGAACAAGTGCTACAGCAGG CCCTGCGGCCCTGAGAAGAGCAAGGGTCCTCTTGATCCCCTCCTTCCCACTGCCCTCCCCTCGCAGCTCA TGACCCTCCCCCACCCCACTGCATCCCTACATGGCACTCTTCGTGTCCCACATACTAACCACAGAGGCCT ATCAAGGTGAAGGCGCTAGTCTTTGACCACTGGTTTGAGGGCAGGGTACATCTTGTTCATGAACAGGCCT GTAAGAGTAGACTCCAGCTTGGATAGGAAGGAACCTTGAATGCTGGCCAAGCTGGCACCCGCCTCACAAG ATTCTCTGTGACCTTCTAAATGTGCTGTTCAGTCTCCTATCTACTACTGTCCACGCAGTTACCCAGCCAT CCCTTGTCTATGTCATCCATCACTTCCGGTTCCTCAGAGAACCTGGCATTCTCTCATGGGTTCACAAAGT GCTCACACCAAGTCCTTGGTGCTGAGGAGTATGGGGGAAGATAGACAGGAAATAAAGACTAGAGTAGAGC CAAACACATGGCACAGACTGGGCTAGCAAGAGTGGATAACAGAGTCTCTGAGGCAAAGTGGCCAGGTGCT GCTGCAACATGTGGAGAAAAGGCAATTGTGTGACCATGAAAAACAGAACCAACCACTTCCAGAGGTCCCT GGGTGGATCTGGAATCCTGCAGGAGTTGCATTAGCCATAAGCTGGGTTCTTATATCTTTCCCATGGCCTG CAATCCAACCTCTGAAGCAGGAGACCTAGCAAGAAAGTTGGGTAGCTGTGCTTCCTCCCCCTCCCCCTGG AAGTTGCCACTACTTTATAATGCCTCCTAACCCCCAATACCCATCTTTTAGGCCCCTTCCCACTACCATG TCCTGTGCCCTCTTTTCAATCTTCACCAGGCCAGATCTCCTGCTGTGTGATCCCCTAAACTGAGGCAGGC CTACAAGGCAGTTAACTTACAAAGAGAAAAAGGATTTTTTTTTTTTTTTTTTTTTTTTGGCTGACCATTC TGGATGTTCCCACGCAAGATAAGGAAGTCGTGTTGCTTTGGGCCTCTGGCAACAATGCCAAGTGGCAATG GCAGGGAGCACATGGCAGAGCAAAATTACACTATGAGCCAGGAAGAAAGAAGAGACGCTTGGACCCAAGT CCTCCAATCCTTCAAGGGTGTGTTCCAGTAGCCTAAAGCCCTTTCCCTAGCTCCACTTCTAAAGGTCCCC TCACCTCCCTGCACAGCTTTGGAATAAGCCTTTAGCCCATCTGCAGCCATAGCAGAGGTCAAAATCGGCT TTCTGGCATTTTGGTCTATGTTTCTTGCATTAAATAAAGATTTTCCTAAATCAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5098655 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5098655 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003072 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source