EMAGE mouse gene expression spatial database

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EMAGE:29611

Coro7 coronin 7 ( MGI:1926135)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_006115_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9637
Entity Detected:	Coro7, coronin 7 ( MGI:1926135)
Sequence:	sense strand is shown >T9637 GCAGGCTGCTGAGGCGGCAGGCTGCAAGAGTCCAGGGCGGCTTCTCTTCATTCACCTAGTACCTGACGAC CCTCGTTCTCCAGCCTGAGCTTGCTCTGTCCCAGCTGCCATGAGTCGCTTCAAGGTGTCCAAGTTTCGGC ATATGGAGGCCCGGCCGTCCCGCCGAGAGGCCTGGATCAGTGATATTCGTGCTGTAACCACCCCCACATG CGGGAACCACATCAAATCAAGCTGCAGCTTGATCGCTTTCAACTCTGACCGCCCAGGTGTATTGGGTGTC ATATCTCTGGAAGGCCATGAGGAAAACAAGAGGCATGTGACCTACCTTGGCTGTCATTCAGACCTGGTCA CTGACCTGGACTTCTCCCCCTTTGATGACTTCCTCCTGGCCTCCGGTTCAGCTGACAGGACGATAAAGCT TTGGCGTCTGTCTGGCACTGGAGAAGCGCTTCCCTCTGTGCCTGGAGTGGTGCTGGGTCCTGAGGAGCTG CCAGTGGAGGTACTGCAGTTCCACCCCACTGTAGACGGTGTTCTGGTGAGCACAGCTGGCAAGACTGTGA AGGTCTGGGATGTAGCCAAGCAGCAGCCCCTGACAGAGTTGGAGGCCCACAAGGACCTGGTGCAGAGTGC TGTTTGGAGCCGGGATGGAGCCATTGTGGGCACAGCATGTAAGGACAAGCAGCTTCGGATCTTTGACCCC AGAGCAAGAACCCAGGCTTCTCAGAGCACACAGGCCCATGAGAACAACAGAGACATCCGGTTGGCATGGA CAGGCATTCAAGAGCACCTTGTGTCCACTGGATTCAACCAGATGCGTGAGCGAGAAGCGAAGCTCTGGGA TACCCGGCTCTTCAGCAGTGCCCTGGCCTCCGTCACCCTGGACACCTCTCCTGGGCCTCTGATACCTCTG CTGGATCCAGACTCTGGGCTATTGGTCCTGGCAGGAAAGGGTGAGAATCAGCTATACTGTTACGAGGTGA CCCCACAGCAGCCTGCACTGAGCCCAGTGACTCAGTGCATTCTGGAGAATGTACTACGAGGAGCAGCCCT TGTTCCCCGGCGAGCACTGGCTGTCATGAGTTGCGAGGTGCTCCAAGTCCTGCAGCTGAGTGACACAGCC ATCATACCCATCAGCCACCACGTGCCCCGAAAAGCTGTAGAGTTCCATGAGGACCTGTTTCCAGACACAG CCGGTTCTGTGCCTGCTTCTGATGCCCACATGTGGTGGGCTGGGGACAACCAGCAGGTTCAGAAGGTCAG CCTCAACCCAGCTCGCCGACCCCACCCTTGCTTCACGTCCTCCCTGGTGCCCACTATGGAACCGGCCCCT GACATGGTCCAGCCTGCAGAGATGCCCAGAGCTGACACAGACCTAAGTGAGGGCTTTTCTTCCCCTAGCT CACTGATGTCACCCTCCACACCATCCAGTCTGGGGCCTTCACTCTCCAGCACGAGCGGCATTGGTACCAG CCCCAGTCAGAGGTCCCTTCAGAGTCTGCTGGGCCCCAGTTCTAAATTTCGCCACACTCAGGGCAGCCTC CTCCACCGCGACAGTCACATCACCAACCTTAAGGGACTCAACCTCACAACACCTGGTGAGAGTGATGGCT TCTGCGCTAACCGGCTGCGTGTGGCTGTTCCCCTGCTCAGCAGTGGTGGGCAGGTAGCTGTGCTTGAGCT GCAGAAGCCAGGCCGCCTGCCTGACACAGCATTGCCTACACTTCAGAATGGGACAGCTGTAATGGATCTG GTCTGGGATCCCTTTGACCCACACCGGCTTGCTGTGGCTGGGGAGGATGCCCGGATCCGACTATGGCGGG TGCCCCCTGGTGGCCTCGAGAATGTTCTCACCACACCTGAGACTGTGCTCACAGGCCACACAGAAAAGAT CTACTCTCTGCGCTTCCACCCCCTGGCTGCCGATGTACTGGCTTCCAGTTCCTATGATCTAACCGTTCGC ATCTGGGACCTTCAGACTGGAGCTGAGCGGCTGAAACTGCAGGGCCATCAGGACCAGATTTTTAGCTTGG CCTGGAGTCCAGATGGAAAGCAACTGGCCACCGTATGCAAGGATGGGCATGTGCGGGTCTATGAACCCCG CAGCAGCCCTTTACCCCTACAGGAAGGCCCAGGACCTGAAGGTGGCCGCGGAGCCCGCATTGTCTGGGTG TGTGATGGTGGTTGCCTGCTGGTTTCTGGCTTTGACAGCCGGAGTGAACGCCAGCTACAGTTGTACATAG CTGACGCCCTGGCACAAGGCCCTTCAGCACTGCTGGGCCTCGATGTAGCTCCCTCGACCCTGCTTCCCAG CTATGACCCTGACACAGGCCTGGTGCTTCTCACTGGCAAGGGTGATACCCGTGTGTTCCTGTATGAGGTG CTCCCGGAGGCCCCTTTCTTCCTGGAGTGCAACAGCTTCACATCACCTGACCCTCACAAGGGTTTTGTCC TTCTGCCCAAGACTGAGTGTGACATTCAGGATGTGGAGTTTGCCCGATGCCTGCGGCTGCGCCAGACCTC CTTGGAGCCTGTTGCTTTCCGGCTGCCCCGGGTCAGAAAAGAATTCTTCCAGGATGATGTGTTCCCGGAC ACAGCTGTGACCTGGGAGCCAGCACTCAGTGCCAAAGCCTGGTTTGAGGGTGCTAATGGACAGCCCCGGC TACTGAGTTTGCAGCCCCCTGGCATGACCCCAGTGAGCCAGGCCCCCCGTGAGGTCCCTGCCCGAAGAGC CCCATCTTCAGCACAATACTTGGAAGAGAAGTCTGACCAACAAAAGAAAGAAGAGCTGCTGAATGCCATG GTAGCAAAGCTGGGGAACCGAGAAGACCCACTGCCACAGGACTCCTTTGAAGGGGTGGATGAAGATGAGT GGGACTAGCCTGCACGCGAGGCACGCCTAATCACAGCTGCCACTGCCACCTCCTACAACTACCTGCTGCC CTCCACACCTAAGATCTCTGCAACCTGGACAATGCTGCACTGGCCCTGGCACGCACATCCAGCTCAGAAG CCCTGGGTGTCACGCAAGCCTCAGGCACTTCCACAGCTGTGGGGCCTCCATGCAGCTGCAGAGACTGCCT GCTCCTTAACCCTCTGGAGCCAACTGTTTATCTTCAGCTGCCTGTTAGCATGTGACACCAGTGGGTGGGA GGTAGGTGTCAGTAGCATGTCTCTGGGCCAACTCGGGATGCCCTGTCTCTCCCAGTCTTTCTGCTGGTTT CCTCTAATATCTTCAGTTACTACAAGCCAAGCTGTCTTGCCTACTGCACCAGCCCCAGTCAAGGACACTG GGGGCTGGTCCCCCACCAAGCTGTTGGGAGCCGCTTTCTCCTTAGGGGCCTAGGACCATTTGTCCTGCTT CTCCTGGCTTGAGGAGAAAGGGTCTCATGGCGCTAACAGCCTCATTACAGTGGTGTGGACCCCATAACAA ACAGCCTATATAGGATATGCTCTGGATGTTTCCAGCAATTAAACTTTTCTAAGCTGGCAAAAAAAAAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6536106 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6536106 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006115 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source