EMAGE mouse gene expression spatial database

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EMAGE:29661

BC017612 ( MGI:3039636)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001782_01	euxassay_001782_02	euxassay_001782_03	euxassay_001782_04	euxassay_001782_05

euxassay_001782_06	euxassay_001782_07	euxassay_001782_08	euxassay_001782_09	euxassay_001782_10

euxassay_001782_11	euxassay_001782_12	euxassay_001782_13	euxassay_001782_14	euxassay_001782_15

euxassay_001782_16	euxassay_001782_17	euxassay_001782_18	euxassay_001782_19	euxassay_001782_20

euxassay_001782_21	euxassay_001782_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4376
Entity Detected:	BC017612, ( MGI:3039636)
Sequence:	sense strand is shown >T4376 CCCACGCGTCCGCACCACCTTCTCTTCTTTACGGAATAACTCAGGTCACGCTGACTGGAAATCAGTTGAC TGACATCAGTGAAGCAGTGGTTCCTGAGTTTGAAGAAAAGAGGTCTCCTGCCTGGACAGAGGAGTGTGTG CCTTTCTCCAAGCCGTCTCTGGACATATCTTCCCAGCATCCTCTAACAAAGATGCGTCAACTCAAAGGGA AGCCAAAGAAAGAGACCTCCAAGGACAAGAAGGAGCGGAAGCAGGCCATGCAGGAGGCGCGGCAGCAGAT CACCACTGTGGTGCTGCCCACCCTGGCTGTGGTGGTGCTCCTCATTGTCGTGTTTGTGTATGTGGCCACA CGCCCCGCCGTCACCGAGTGAGCACGTCCATTCTGCTTTCCTCTGCAGAGATGTCCGAAATGCCAAGCTC TGAGCAGGAGTGCCCTGTCTCCTCTGGTCTTTGACTTGATCAATATTTCTCTACTTTTTTGGGGGGTGGG GTGGGGTGGGGATAGGGAACATTTTATCAGTGAATGTGCCATTTACTTGATGGTCCACTTCATGTGCCTT TCAGACTTCAAAGCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGGCCCTTTTTCTCCTGAAATCGA TGCATCGCTGCCCCTGAGGAGAGCCTCTCTGGGTGATGCTGTTGGGATCAGAGTCGCCCTCCCATCCAGC AGCATGCTTCCCGGCTCCACTTCACAACTTTCTAAGGGGACAGCCTTTCTTCTCTGTGCTTGATGGGATT TGAAATATGTTGACTCAAAGTGAAATATTTATTTTTTGAATAAAGGAGATAATAGTCTTAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4911985 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4911985 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001782 same experiment
	EMAGE:30846 same embryo
	EMAGE:29992 same embryo
	EMAGE:29658 same embryo
	EMAGE:30049 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source