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EMAGE:29687

Spaca4 ( MGI:1916613)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:29687
euxassay_003943_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9755
Entity Detected:Spaca4, ( MGI:1916613)
Sequence:sense strand is shown

>T9755
GTCACTGAGTTCCATGGCCCAAAGGCTTGTGAGCAGGGGTGCTGGCCCATCTCCAACCCGAGGACTGAAG
AGCTTCTGAAAAGGACACGTTGTATCGGGAACCCGAAACACTCCAACTGCTGACCCCCAAGGCCAGGACG
AGGGGCAGGAGGTGTTCCAGGAGGTCACACTCGGCCATCCAGGAGGTCAAGGAAGCCCCCGGCCTGGCTT
GGGTGCTATCCACGCCGCCAGGCCGCCATGGTCCTTGGCTGGCCACTGCTTCTGGTGTTGGTTCTTTGCC
CAGGTGTGACAGGCATCAAGGACTGCGTCTTCTGTGAGCTGACTGACTCTGCTCGGTGCCCTGGCACACA
CATGCGCTGTGGGGATGACGAAGATTGCTTCACAGGCCACGGAGTAGCCCAGGGTGTGGGGCCCATCATC
AACAAAGGCTGCGTGCACTCCACCAGCTGTGGCCGCGAGGAACCCATCAGCTACATGGGCCTCACATACA
GTCTCACCACCACCTGCTGTTCTGGCCACCTTTGCAATAAGGGCACTGGCCTTTCCACAGGGGCTACCAG
CCTGTCACTGGGTCTGCAGCTGCTCCTGGGCCTGTTGCTGCTGCTTCAATACTGGCTGTGACCCACAAGA
ACTGCAGGGCCTAGGGCTGTGCCCCGGGCTTCCGCCTTCATCCCTTTCCCCCATTAAATGGCCAGAGGTG
CAGTCTAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6742046 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6742046 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_003943 same experiment
 EMAGE:29684 same embryo
 EMAGE:29665 same embryo
 EMAGE:30633 same embryo
 EMAGE:32212 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS