EMAGE mouse gene expression spatial database

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EMAGE:29724

Pole4 polymerase (DNA-directed), epsilon 4 (p12 subunit) ( MGI:1914229)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3637
Entity Detected:	Pole4, polymerase (DNA-directed), epsilon 4 (p12 subunit) ( MGI:1914229)
Sequence:	sense strand is shown >T3637 CGGCTGCTGGGAGCGGGACGCCCAGAGAGGAAGAGGCTCCCGGAGGGGAGGCAGCGGCTTCGCAGGCCCA AGCCCCGACGAGTGCACCCGGGGGAGTTCGCCTCTCGAGGCTGCCTCTGGCGAGAGTAAAGGCCTTGGTG AAGGCAGACCCTGACGTAACGCTGGCGGGACAGGAAGCCATCTTTATCCTGGCGCGTGCCGCGGAACTGT TCGTGGAGACTATCGCAAAAGATGCCTACTGCTGTGCTCAACAAGGAAAGAGGAAAACTCTCCAGAGGAG AGATTTGGATAATGCAATAGAAGCTGTGGATGAATTCGCTTTTCTAGAAGGCACTTTGGATTAATTGTGG AGCTGGATGGTCTGAGCCTTGGCTGCATCTGTCACCTTTCCCCTCTGTAAGCCTCAGCTTTGGAGAAAGT CTGCGCATGTACACAATCTTCTTACTCTTTTTACCCAAGATAGAAGAAGCAGAGAGCTCTTGGATAACGT TCTCCACCAAGTCTTCCATTACTTGAGTCTGTCTTCCAATTCAAGCCTGG
Notes:	The probe template was PCR amplified from IMAGE:3168606 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3168606 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000419 same experiment
	EMAGE:29807 same embryo
	EMAGE:29791 same embryo
	EMAGE:29806 same embryo
	EMAGE:31771 same embryo
	EMAGE:29669 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source