Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:29730

Fyco1 FYVE and coiled-coil domain containing 1 ( MGI:107277)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:29730 EMAGE:29730 EMAGE:29730 EMAGE:29730 EMAGE:29730
euxassay_010203_01 euxassay_010203_02 euxassay_010203_03 euxassay_010203_04 euxassay_010203_05
EMAGE:29730 EMAGE:29730 EMAGE:29730 EMAGE:29730 EMAGE:29730
euxassay_010203_06 euxassay_010203_07 euxassay_010203_08 euxassay_010203_09 euxassay_010203_10
EMAGE:29730 EMAGE:29730 EMAGE:29730 EMAGE:29730 EMAGE:29730
euxassay_010203_11 euxassay_010203_12 euxassay_010203_13 euxassay_010203_14 euxassay_010203_15
EMAGE:29730 EMAGE:29730 EMAGE:29730 EMAGE:29730 EMAGE:29730
euxassay_010203_16 euxassay_010203_17 euxassay_010203_18 euxassay_010203_19 euxassay_010203_20
EMAGE:29730
euxassay_010203_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36402
Entity Detected:Fyco1, FYVE and coiled-coil domain containing 1 ( MGI:107277)
Sequence:sense strand is shown

>T36402
CCTTTGAACCCTTCTACCCTCTTGCAAATGGAATGCTGAAGCCAGCTTGTTCAATAGATCAACAGCATCC
AGTACCAAATAGGATCTCATTTTTATTGCTTGGATTTTCCAGGGTATATTGGAGACACTGGCCAAACTCA
TGGCCTTCTGCTGGTGTCACTGCCACCATCTTCATCAAGAGCCATCATTCACTGAGTTGCTGCTGTGTTG
GTGGCCTGGGAGGGGCCTGAGACCTTACATTTGAGTTCATCCTGAACTCAGTCACTCATCAGGAGGCCCA
TGTGGTTCTTGTGAAGCTCAGACCTGAGGTGAGAGGCTCCAGTGCTGACTGCATAGTACTCCACTAGGCA
TATACCAACTCTGTTTTTAATAGTCTATTTTTGTGCTAAATGGGCTTTGTACTGTACTTGCTCCAGTCTG
CTGAGCACTAGGTTACTAGAGAACATTTGGCCCTGTGTAAGTGTGCCTTTCATACATAGCTTTATGGTTT
TAAGTAGTCCCCAGTTTTACTGGGGTTTAGTCATTGGAGTAAATTTTTGAAAAATGCAATTGACAACTGA
AGAATCATTAAGGAAGATGATGGCCCAGCTGGATTTAATTAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 92809. Forward Primer - name:092809_F_cDNA_Fyco1, sequence:CCTTTGAACCCTTCTACCCTCT; Reverse Primer - name:092809_N_SP6_cDNA_Fyco1, sequence:GTAATTAAATCCAGCTGGGCCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_010203 same experiment
 EMAGE:29727 same embryo
 EMAGE:29733 same embryo
 EMAGE:29732 same embryo
 EMAGE:29735 same embryo
 EMAGE:29734 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS