Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:29816

2310028H24Rik ( MGI:1919151)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:29816 EMAGE:29816 EMAGE:29816 EMAGE:29816 EMAGE:29816
euxassay_015861_01 euxassay_015861_02 euxassay_015861_03 euxassay_015861_04 euxassay_015861_05
EMAGE:29816 EMAGE:29816 EMAGE:29816 EMAGE:29816 EMAGE:29816
euxassay_015861_06 euxassay_015861_07 euxassay_015861_08 euxassay_015861_09 euxassay_015861_10
EMAGE:29816 EMAGE:29816 EMAGE:29816 EMAGE:29816 EMAGE:29816
euxassay_015861_11 euxassay_015861_12 euxassay_015861_13 euxassay_015861_14 euxassay_015861_15
EMAGE:29816 EMAGE:29816 EMAGE:29816 EMAGE:29816 EMAGE:29816
euxassay_015861_16 euxassay_015861_17 euxassay_015861_18 euxassay_015861_19 euxassay_015861_20
EMAGE:29816 EMAGE:29816 EMAGE:29816
euxassay_015861_21 euxassay_015861_22 euxassay_015861_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40524
Entity Detected:2310028H24Rik, ( MGI:1919151)
Sequence:sense strand is shown

>T40524
TGGTCGTCCCTAATAAAGGCTACTCCTCGCTGGACCAGAGCCCTGATGAGAAGCCACTGGTAGCTCTTGA
CACAGACAGCGACGATGACTTTGACATGTCCAGATACTCTTCCTCTGGCTACTCCTCTGCCGAGCAGATC
AACCAAGATTTGAACATCCAGCTGCTGAAAGATGGCTACCGGTTAGACGAGATCCCCGACGACGAGGACC
TAGATCTCATCCCCCCCAAGTCTGTGAACCCCACCTGCATGTGTTGTCAGGCCACTTCCTCCACCGCCTG
CCACATTCAGTAGTGGGCGGGGTCGCCGCGGTGCACGGGGCGCTGACCTGGGCCCGGTGGGATGCAGCAG
GAGAGACCCCTACCCTGCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 162147. Forward Primer - name:162147_F_cDNA_mCG9749.3, sequence:TGGTCGTCCCTAATAAAGGCTA; Reverse Primer - name:162147_N_SP6_cDNA_mCG9749.3, sequence:AGCAGGGTAGGGGTCTCTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_015861 same experiment
 EMAGE:29818 same embryo
 EMAGE:29297 same embryo
 EMAGE:29854 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS