EMAGE mouse gene expression spatial database

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EMAGE:29931

Kcnq1 ( MGI:108083)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002123_01	euxassay_002123_02	euxassay_002123_03	euxassay_002123_04	euxassay_002123_05

euxassay_002123_06	euxassay_002123_07	euxassay_002123_08	euxassay_002123_09	euxassay_002123_10

euxassay_002123_11	euxassay_002123_12	euxassay_002123_13	euxassay_002123_14	euxassay_002123_15

euxassay_002123_16	euxassay_002123_17	euxassay_002123_18

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5516
Entity Detected:	Kcnq1, ( MGI:108083)
Sequence:	sense strand is shown >T5516 GCTCTGCGCTGCCTTCACCTCAGCTCCGAGGAGCAGCGGGGTCAGGGGTCCTGTCTGGCCATGGACACGG CCTCGTCCCCGCCCAGTGCTGAAAGGAAGCGCGCGGGTTGGAGCCGCCTGCTAGGCGCCCGGCGGGGCAG CGCGGTGGTCAAGAAGTGTCCCTTCTCACTGGAGCTGGCCGAAGGTGGCCCTGAGGGCAGCACGGTCTAT GCGCCCATCGCGCCAACCGGAGCCCCCGGGCTCGCGCCCCCCATGTCGACCCCAGTGTCGCCCGCCCCGG CCCCTGCAGACCTCGGCCCACGTCCGCGGGTGAGCCTTGACCCGCGGGTCTCCATCTACAGTGCGCGCCG CCCGCTGCTGGCGCGCACCCACATCCAGGGCCGAGTCTACAACTTCCTCGAGCGCCCCACGGGTTGGAAG TGTTTCGTGTACCACTTCACCGTCTTCCTCATTGTTCTGGTCTGCCTCATCTTCAGTGTCCTGTCCACTA TTGAGCAGTATGCCGCTCTGGCCACCGGGACCCTCTTCTGGATGGAGATTGTCCTTGTGGTGTTCTTTGG GACAGAATATGTGGTCCGCCTCTGGTCTGCAGGCTGCCGCAGCAAGTACGTGGGCATCTGGGGCCGGCTA CGTTTTGCCCGGAAGCCCATTTCCATCATTGACCTCATCGTGGTTGTAGCCTCTATGGTTGTCCTCTGCG TGGGTTCCAAAGGACAAGTGTTCGCCACATCAGCTATCAGGGGTATCCGCTTCCTTCAGATCCTGCGGAT GCTGCATGTCGATCGCCAGGGGGGTACCTGGAGGCTCCTGGGCTCTGTAGTCTTCATTCACCGCCAGGAG CTGATCACCACCCTGTACATTGGCTTTCTGGGCCTTATCTTCTCCTCCTACTTTGTCTACTTGGCTGAGA AAGATGCGGTGAACGAGTCCGGCCGCATCGAGTTTGGCAGCTACGCAGATGCTCTGTGGTGGGGGGTGGT CACAGTCACTACCATTGGCTACGGGGATAAGGTACCTCAGACGTGGGTTGGGAAGACCATCGCCTCCTGT TTCTCTGTCTTCGCCATATCCTTCTTTGCACTCCCAGCGGGGATACTTGGCTCTGGGTTCGCGCTGAAGG TCCAGCAGAAGCAGAGGCAGAAGCACTTCAACCGGCAGATCCCAGCTGCAGCCTCACTCATCCAGACTGC ATGGAGGTGCTATGCCGCTGAGAACCCTGACTCAGCCACTTGGAAGATCTATGTCCGGAAGCCTGCTCGG AGTCACACGCTTCTGTCCCCCAGCCCCAAACCTAAAAAGTCTGTCATGGTAAAGAAGAAGAAGTTCAAGC TGGATAAGGATAATGGGATGAGTCCTGGAGAGAAGATGTTCAATGTTCCTCACATCACTTATGATCCCCC AGAGGATAGGAGGCCAGACCATTTCTCCATTGATGGCTATGACAGCTCAGTAAGGAAGAGCCCTACACTG CTGGAAGTAAGCACACCCCATTTCTTGAGAACAAACAGCTTTGCAGAGGACCTGGACCTGGAAGGGGAGA CACTGCTGACCCCCATCACCCATGTGTCACAGCTGCGGGATCACCATCGGGCCACCATCAAGGTCATCAG GCGCATGCAGTACTTTGTAGCCAAGAAGAAATTCCAGCAAGCACGGAAGCCCTACGACGTGCGAGATGTC ATCGAGCAGTACTCCCAGGGCCACCTGAACCTTATGGTGCGCATTAAAGAACTACAGAGAAGGCTGGATC AGTCCATTGGGAAGCCATCTTTGTTCATCCCCATCTCAGAAAAGAGCAAAGACCGTGGCAGTAACACCAT CGGTGCCCGTCTGAACAGGGTGGAAGACAAGGTGACACAACTGGACCAGAGACTGGTGATCATCACAGAC ATGCTCCACCAGCTGCTGTCCATGCAACAAGGTGGTCCAACCTGCAACAGCAGGTCACAAGTTGTAGCCA GCAATGAAGGTGGCTCCATCAACCCTGAGCTCTTCCTACCCAGCAACAGCCTGCCCACCTACGAACAACT GACTGTGCCCCAGACAGGCCCTGATGAGGGTTCCTGAAGGAGCTCAGAAGGGAGGTCCAGGGTAAACCCC CCACAGGTCTGCCCCACCTCCCTCTCAACAGGGGCACACCCACCTGGTTCTCACCCCCTTGAAGGCCTGA TATGACAGCCTAGCTCCCTAAGGCCCCGAAACCCATGGGCCAAGCCACTGGCCTGGATCTGGATATCACC AACACCCCTCTGCCCAGCCAGTGGGAACTAGAACTTGTCTGGGCATGGGGTTCCTCTCAGGCCCCTATCA AGATGTTGATGCTGCTCTTCTGATGGCTGGAACATAGGGATGGGGAGTATATGTGTAAAGCAGGATGCAG CTCAGGCAGAGGGAAGCCCATCCAGGTATGCCAAGTGGCTTCCTAAAGGGAAACAGGAAGAGTCTGGGGT TTCCACCCTCAGCCCTGAATCCAGAGACCATCAGGGAACGTGCAGGGCAAGACAGGCCCGGCCCACACTA ACTGCAGGCTGTAGCCCAGTGGGAGCTCAGCAGGCTAGAGCCTGACTCCTTCATTCTCTAGTGATGTTGA CCACAACAAGATACTAGCGGGATTTTCACACCCCCCCCAAAGGATATCCCAAGCAAGGGATTGTCCACTG TCCACCCTAGCCAGGTATGGACTGACAACTTCTCCCCTAAAGTGAAAAGCAGGGCCCTCAAGCCCTATGA GCCCCACATGTGCAGCCTGCCTCCTTCTTGCTGGTCAGAGGTGCTGGACCAATGCTCAGACTCCAGCCTC TTTCACGCCACATTGAAGTTAGGGGCATCACCGATTCTTCCAGGATTGGTTCCTTGGGCTTGGGCACCAC GCCTCACAGAAATCAAGATAATTTCTGGTGATTCAGACTCTTGTGTTTTAATGAATTTCGTAGTATGATC TTGATTGAGAGACTTTTCCAAATAAAGTTGGGACGTCCCAGGTTAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6397978 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6397978 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002123 same experiment
	EMAGE:29934 same embryo
	EMAGE:32242 same embryo
	EMAGE:31929 same embryo
	EMAGE:29774 same embryo
	EMAGE:29944 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source