EMAGE mouse gene expression spatial database

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EMAGE:29950

AU018778 ( MGI:2142687)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_006266_21	euxassay_006266_22	euxassay_006266_23	euxassay_006266_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7533
Entity Detected:	AU018778, ( MGI:2142687)
Sequence:	sense strand is shown >T7533 GTCCATGCAAGATGTTCCTTAGCACTCTGTTCCTGGTGTCTCTAGCAACCTGTGTGATTTGCGGAAATCC CTCTTCACCACCTGTGGTAGACACTGCTCATGGTAAAGTCCTGGGGAAACACGTGAACGTAGAAGGATTT TCACAGCCTGTGGCCGTCTTCCTGGGAATCCCCTTTGCCAAGCCCCCTCTTGGATCCCTGAGGTTTGCTC CACCACAGCCTGCAGAGCCCTGGAGCTCAGTGAAGAATGCCACCACCTACCCACCTATGTGCTCCCAAGA TGCAGCTAGAGGACAGGCGGTCAATGACCTCATAACCAATAGAAAGGAGAAAATCCATCTTGAATTTTCT GAAGATTGCCTGTACCTAAATATTTACACTCCTGCGGACTTTTCAAAGAACAGTAGGCTACCGGTGATGG TGTGGATCCATGGAGGTGGACTGAAGCTGGGTGGGGCATCAAGCTTTGATGGACGGGCTCTCTCTGCATA CGAAAATGTGGTGGTGGTGGCCATTCAATATCGCCTGAGTATCTGGGGATTCTTCAGCACAGGGGATGAA CACAGTCGGGGAAACTGGGGTCATTTGGACCAAGTGGCTGCTCTGCATTGGGTCCAGGACAACATTGCCA ACTTTGGCGGGGACCCAGGCTCTGTGACCATCTTTGGAGAGTCAGCAGGAGGTTACAGTGTCTCAATTCT TATATTGTCCCCATTGTCCAAGAACCTCTTCCACAGGGCCATTTCTGAGAGTGGTGTGGCCTTCATTCCT GGAATGTTTACCAAAGATGTGAGGCCAATTACTGAGCAAATTGCTGTTACTGCTGGCTGTAAGACCACCA CGTCTGCCGTCATTGTTCACTGCATGCGCCAGAAGACGGAGGAGGAGCTATTAGAGATCATGCATAAATT GAATCTGTATAAACTGAGTTTACAAGGAGATACCAAAAATAGCGACCAGTTCGTGACAAGTGTGCTTGAT GGAGTGGTGCTACCAAAGGACCCCAAAGAGATCCTGGCTGAGAAGAACTTCAACACTGTGCCTTACATTG TGGGAATCAACAAGCAAGAATGTGGCTGGCTTCTGCCAACAATGACGGGATTTCTACCAGCTGATGTAAA ATTGGACAAGAAGAAAGCCATTGCACTCCTGGAGCAATTTGCTTCCATGACTGGCATACCAGAGGATATT ATTCCAGTTGCTGTTGAGAAGTACACAAAAGGTAGTGATGACCCTGATCAGATCAGAGAGGGAGTTCTCG ACGCAATGGGGGATGTGGCATTTGGTGTTCCATCGGTGATTGTGTCCCGTGGCCACAGAGACACTGGAGC TCCCACCTACATGTATGAGTATCAATACTACCCAAGCTTCTCATCACCCCAAAGACCCAAGAATGTAGTA GGAGACCATGCAGATGATGTCTACTCTGTCTTCGGTGCTCCAATTTTAAGAGAGGGTGCCTCCGAAGAGG AGATCAATCTCAGCAAGATGGTGATGAAATTCTGGGCCAACTTTGCTCGGAATGGGAACCCTAATGGCAA AGGGCTGCCTCATTGGCCAAAGTATGATCAGAAAGAAGGATATCTTCATATTGGTGGCACCACCCAGCAA GCCCAGAGACTGAAGGAAGAGGAAGTGACTTTCTGGACACAGTCCCTTGCCAAGAAACAACCCCAGCCAT ACCACAATGAGCTGTGAATGCAAAGCTCTGCCAGGCTTATACCAGAGAAGCCAAGATATGGTTCTTCCAC TAACAGTGTATGTAAATCCAAGATGGATCTTGGAGGATCCTGAAGAACTTTGTCATAGATATAGGCAGAG CCCAGGGAAGGGAAATGTTGTATTTATGTCTTTAATTTAGAGAATAAATGTTCTTTATATGGTCAAAAAA AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4239756 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4239756 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006266 same experiment
	EMAGE:31895 same embryo
	EMAGE:31785 same embryo
	EMAGE:31502 same embryo
	EMAGE:31535 same embryo
	EMAGE:31897 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source