EMAGE mouse gene expression spatial database

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EMAGE:29969

0610039P13Rik ( MGI:1921346)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_004060_11	euxassay_004060_12	euxassay_004060_13	euxassay_004060_14	euxassay_004060_15

euxassay_004060_16	euxassay_004060_17	euxassay_004060_18	euxassay_004060_19	euxassay_004060_20

euxassay_004060_21	euxassay_004060_22	euxassay_004060_23	euxassay_004060_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4383
Entity Detected:	0610039P13Rik, ( MGI:1921346)
Sequence:	sense strand is shown >T4383 CAGAGCTAAGCAGACTGCTGCCAGGTGGAGAGAGCCAGCCAGCCACTGCAAAGGAGTGCTGCAAACTACC AGCAGGTACTGCTCAAACGGACCTTCGAGGACAGATTGCGGAGCGTCTACTCGAGACAGCAGGGTGACAA GTCTGCCTCCAGTTCTCTCACTGTTGGATCTTGAGACAACAGGAAAATGACTTCCCATGACCCAAAGGCC GTCACTCGCAGAACCAAGGTGGCTCCCACCAAGAGGATGAGCAGGTTCTTGAAACACTTTACGGTGGTTG GGGACGACTACCACACGTGGAATGTCAACTACAAGAAGTGGGAGAATGAGGAGGAGGAGGAGGAGCCAGC GCCCACATCAGCAGAGGGTGAGGGCAATGCTGAGGGCCCAGATGCCGAGGCTGGCTCTGCCTCCACGCCC AGGCAGTCCCTGGACTTCAGGAGCCGACTGAGGAAACTCTTCAGTTCCCACAGGTTTCAGGTCATCATCA TCTGCCTGGTGGTCCTGGACGCCCTCCTCGTGCTTGCTGAACTCCTCCTGGATTTGAAGATCATCGAGCC GGACGAGCAAGACTATGCGGTCACGGCGTTCCACTACATGAGCTTTGCCATCCTGGTCTTCTTCATGTTG GAGATTTTTTTCAAGATCTTCGTCTTCCGCTTAGAGTTCTTCCACCACAAGTTTGAGATCCTGGATGCCT TCGTGGTGGTGGTGTCTTTCGTCCTTGACCTTGTCCTCTTGTTTAAAAGCCACCACTTCGAAGCTCTAGG GCTGCTGATCTTGCTTCGGCTCTGGAGGGTGGCCCGGATCATCAATGGCATCATCATCTCCGTGAAGACA CGCTCAGAACGGCAGATCTTAAGGCTAAAGCAGATAAATATCCAACTGGCCACCAAGATCCAGCATCTGG AATTCAGCTGCTCCGAGAAGGAACAAGAAATTGAGCGGCTCAACAAGCTGTTGAAACAGAATGGACTTCT CGGGGACGTGAACTAGCCTTCCAGACACTCCGCCTGGAGTAGCTGGGCTGTGGCCAGAGAGCCAGCCTGT GGTGCAAAGTCCAGTCTCCATCTCTGCCTTGGCTTGCACCCCGGGATGCTGGGGACACATCTTCACTGGT ACTGCTGTCTCCTTGGCGTCCACGCCCTACGCTTGCAGCAAAGGGATCATCGCACCAACCTTTTCTCAAG CTGGACTCCACTGTCGCTCAAAAATGATCAAGGAGGCTGGGCTTGTAAAGCCAACTTAATGCACATCCTG AAAGGAGCTGGAGCCCGCACCCCGAACCAGCTCACTCTGGAAAACCTTTCTCTACTAGAATGTCCCCAGC TTCTCCTGCTGCCTCCCTAGGGGAGCCATCATGGTACCCAGTCCCTCCTCTGCAGCATCCAGCTGTACCA TCAGCTTCAAACTGGCTCTGTACCCACTAGCAGCTCTTTCAGTTACTGTACAATTAATGTGTAAAATAAG CCCTCTCCTTCCCAGGGCTCACCCACCACATTCTCTCCTCGGTGGCAGCCCACACCAGCCCACCAGTTAT ACAGCCAGCACATGGCAGGGTCCCAAGGCTGCCCTGAGGGCCCTGTAATTAGCACAATCTTGGGATACAA CCCCCTTGTGGGCACACAGTTTTACATAGCTCCTTGCTGGTTTGTACCTCTCAAATGCCAACAGGCATTC TGAAAACAACTCCCACTAGACCAGGGATGATGCACTGACGAGGAGGCTCTCCATGCACTGCCACGTCACA CTCTGTTCCCGCTCATCTCCCAGAGGGCCGTGCCCTTTGACCTGTATGTGCAGGGTGCTGGACCCATCTG ACCCAACACCACAGCCCAGCAGAGTAAAGTTTCTGAGAGTTTTAAAAGGCAGTGGAGTAGAGCAGAGGGC TGCTTGAAGGGTAACAGCAAGGGGAGGCGGCAGCTAGGTTCATGTCCTCTTGCGCCTAGGGCCCAGGTGC GCACTGCCGCGGAAGGGAAGATCTTCAGGATGGGTACTCCTGCGGGGTCAGCTCATCACACAAACGGGAA GAAGAAGCTGAAGGGTAGCCTGGCGTTGATGGTGGGTGGGGCTCGGAAGCCTGCCTCTGCAGGGGCTGAC ACATCCACAAATGTCACTGCAGTGGAAATGACCACGGTCCTCTCAGGCCCTGGGAGGGGCTGCAAGGAAA AGACAGAGTGGACACCCTCACCATCTCCTCGCTCACAGCCAGCTGCCCTAAGGCAGGCACTAAGGCGGCT GAGGAGCGCTCTGCCGCAGACCACGCTGGTACAGCAGAGCTGGTCCAACCACAGTTCAGTGCTCACAAAT AAAACCTGTCAGGCTTTTCCTCTAAGTACAAGTTAAAGAATTATCATGAACTTTTTTAAGACTCAAAATT AAGTTAGGCATGATTGATGGCACATGCCTGTTAATGCTCAGGGGCCTGAGGCCGAGGGTCAGTTTGAGAC CAGCCTGGGCTCCATGAGAAGACCCAGCAGGCAAAGCAGCACGTGGGAGCTGTGAGGACGACCCCAGCCC CAATACCCCCTTGTAAAGGAACTGAAATCTAGAGAGAAATAACCTTTCATTCCAAGTAATAAACCTTTCC CACCACCCTTGCTGTCATGGGGCTTTCTGTAACAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4913027 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4913027 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004060 same experiment
	EMAGE:30869 same embryo
	EMAGE:30019 same embryo
	EMAGE:30890 same embryo
	EMAGE:30882 same embryo
	EMAGE:29984 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source