EMAGE mouse gene expression spatial database

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EMAGE:30049

Hr ( MGI:96223)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004031_01	euxassay_004031_02	euxassay_004031_03	euxassay_004031_04	euxassay_004031_05

euxassay_004031_06	euxassay_004031_07	euxassay_004031_08	euxassay_004031_09	euxassay_004031_10

euxassay_004031_11	euxassay_004031_12	euxassay_004031_13	euxassay_004031_14	euxassay_004031_15

euxassay_004031_16	euxassay_004031_17	euxassay_004031_18	euxassay_004031_19	euxassay_004031_20

euxassay_004031_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5548
Entity Detected:	Hr, ( MGI:96223)
Sequence:	sense strand is shown >T5548 GAGTGGTCCAGCAGGCACGGGCCTGCTGGCGTGGGTCGCAGCACCCATCACTGACCCGGTGCTGGAGGAG GAAACCGGCGGAGCAGCTTCCCCACTCTCAGTTGCGCGTCTGGCGATGGCGATCAGAGGTCCTGCTGCGC TCTCCGCTGCGCTCTCCCTCCATTAGCCGCGCTACGCGGTGCTGCGCCCTCGCTGGTGCCTCGCTCCTGG CTCACAGGATCGGCCCCCCACTTCCAGGCACGACCCCCTTCCCCGGCCCCTCGGCCTTTCCCCCCACTCA GCCATCTCCGACCCGGGGCGCGCGTTCCCCCCGGCCCGGATCCTTCTCTCCCTCCGGGGACACCCGCTCC CTAGCCCCGGCTCGACCCTCCCCAAAGCGCAGCACGGAGTCTCGGCGTCCCATGGCGCAACCCACAGCCT TGGCCCAGAAGCTGGTGCGGCCCATCCGCGCCGTGTGCCGCATCCTGCAAATTCCGGAGTCCGACCCCTC CAACCTGCGGCCCTAGAGCGCCCCCGACGCCCGGGGGGAAAGAGAGCGCGAGAGCGCTGAGCAGAAAGCG GGAGAACGCCCCGAAGCCGGCCCATGGGGAGCGAGTACCCAGCACCGGCTAGCACGACCGCCGCCCACAC CGCGCCCGGCCTGTGAAGCCCAGGACCCCCCTCCCCGGGAGAGGCCCATGAGGGCAGGAGAGTGATGGAG AGTATGCCCAGCTTCCTGAAGGACACCCCAGCCTGGGAGAAGACAGCCCCTGTGAACGGCATTGTGGGAC AGGAGCCTGGTACCTCACCACAGGATGGCTTGCGCCATGGGGCACTGTGCCTAGGAGAACCTGCTCCCTT TTGGAGGGGTGTTCTGAGCACCCCAGACTCCTGGCTCCCTCCTGGCTTCCTCCAAGGCCCCAAGGACACA CTCTCACTGGTGGAGGGTGAGGGTCCTCGGAACGGGGAGAGGAAGGGCAGCTGGCTAGGCGGCAAGGAAG GACTGCGCTGGAAGGAAGCGATGCTGGCCCATCCACTGGCCTTTTGTGGGCCGGCATGCCCACCTCGCTA TGGGCCCCTGATACCTGAGCATAGTGGTGGCCATCCCAAGAGTGACCCTGTGGCCTTCCGGCCCTTGCAC TGCCCTTTCCTGCTGGAGACCAAGATTCTAGAGCGAGCTCCCTTCTGGGTTCCTACCTGCTTGCCTCCCT ACCTGATGTCCAGCCTGCCCCCAGAGCGTCCATATGACTGGCCTTTGGCCCCAAACCCATGGGTATACTC TGGGAGCCAGCCAAAAGTGCCCTCTGCCTTTGGCTTAGGCAGCAAGGGCTTTTACCACAAGGATCCGAAC ATCCTCAGGCCAGCAAAGGAGCCCTTGGCAGAGTCTGGGATGTTGGGCTTAGCCCCTGGTGGGCATCTCC AGCAAGCCTGTGAATCAGAAGGCCCCTCACTTCACCAGAGGGATGGGGAGACAGGAGCTGGCAGGCAGCA GAATCTTTGCCCAGTTTTCTTGGGGTACCCAGACACTGTTCCTCGGGCCCCCTGGCCTTCTTGTCCCCCA GGCCTGGTTCACAGTCTTGGCAACATTTGGGCTGGGCCAGGAAGTAATAGCCTTGGGTACCAACTAGGAC CACCAGCCACACCAAGGTGCCCATCTCCTGGGCCTCCTACCCCTCCAGGGGGCTGTTGCTCATCCCATCT ACCTGCCAGAGAGGGGGATCTTGGCCCTTGTAGGAAATGCCAGGATAGCCCAGAGGGAGGTAGCAGTGGG CCAGGGGAATCCAGTGAAGAAAGGAACAAAGCTGATTCCAGGGCCTGTCCCCCAAGCCATCACACCAAGC TGAAAAAGACCTGGCTCACACGCCACTCGGAACAGTTTGAGTGCCCAGGTGGCTGTTCAGGGAAGGAAGA GAGCCCAGCCACTGGGCTCCGGGCACTCAAGAGGGCAGGCAGTCCAGAGGTCCAAGGAGCATCAAGGGGC CCAGCTCCCAAACGCCCATCCCACCCTTTCCCAGGCACTGGGAGGCAGGGGGCCAGGGCTTGGCAGGAGA CACCGGAGACAATCATAGGAAGCAAGGCGGAGGCAGAGCAACAAGAGGAACAGAGAGGGCCCCGAGATGG CAGGATTAGGCTCCAGGAGTCCAGACTTGTGGATACATCCTGCCAGCATCACTTAGCAGGTGTCACCCAG TGCCAAAGCTGTGTCCAGGCAGCTGGAGAGGTAGGGGTACTGACCGGCCACTCCCAGAAATCACGTAGGT CACCCCTGGAGGAGAAGCAGTTGGAGGAGGAGGATTCCTCTGCCACTTCCGAAGAAGGAGGAGGAGGGCC TGGCCCAGAAGCTTCACTCAACAAGGGCCTGGCCAAGCACCTGCTGAGTGGTTTGGGGGACCGACTCTGC CGCCTGCTGCGGAAGGAGCGGGAGGCCCTTGCCTGGGCACAGCGAGAAGGCCAGGGGCCAGCCATGACAG AGGACAGCCCAGGCATTCCACATTGCTGCAGTCGATGCCACCACGGACTCTTCAACACCCACTGGAGATG TTCCCACTGTAGCCACCGGCTGTGTGTAGCCTGTGGTCGCATAGCCGGTGCTGGAAAGAACAGGGAGAAA ACAGGTTCTCAGGAACAGCACACAGATGACTGCGCCCAGGAGGCTGGGCATGCTGCCTGTTCCCTGATCC TGACCCAGTTTGTCTCCAGCCAGGCGCTGGCAGAACTGAGCACTGTGATGCACCAAGTCTGGGCCAAGTT TGACATTCGGGGGCACTGTTTCTGCCAGGTTGATGCCCGTGTGTGGGCCCCCGGGGATGGGGGTCAGCAG AAGGAACCAACAGAGAAAACTCCCCCAACTCCACAACCTTCCTGCAATGGAGATTCCAATCGGACCAAGG ACATCAAAGAAGAGACCCCAGACTCCACTGAGAGCCCAGCAGAGGATGGTGCTGGCCGGTCACCCCTTCC TTGTCCCTCTCTCTGTGAGCTGCTAGCCTCTACTGCTGTCAAACTCTGCCTGGGGCATGACCGGATTCAC ATGGCCTTTGCTCCGGTCACCCCAGCTCTGCCCAGTGATGACCGCATTACCAACATCCTGGACAGCATTA TTGCGCAGGTAGTAGAACGGAAGATCCAAGAGAAAGCCCTGGGGCCAGGCCTGCGAGCAGGGTCAGGCTT ACGCAAGGGCCTGAGCCTTCCATTGTCACCAGTGCGAACCCGGCTGTCTCCTCCTGGAGCTTTGCTGTGG CTGCAGGAGCCCAGGCCTAAGCATGGCTTCCATCTCTTCCAGGAACACTGGCGGCAGGGCCAGCCCGTGT TAGTGTCAGGCATCCAGAAGACATTGAGACTTAGCCTGTGGGGAATGGAAGCCCTTGGGACACTTGGTGG CCAGGTGCAGACACTGACTGCCCTTGGGCCTCCCCAGCCCACAAACCTGGACAGCACAGCATTCTGGGAG GGATTCTCTCATCCTGAGACACGTCCAAAGTTAGATGAGGGCTCTGTCCTCCTGCTACACCGAACCCTGG GGGATAAGGACGCTAGCAGGGTGCAGAACCTTGCCTCCAGCCTTCCACTCCCAGAATACTGTGCCCACCA AGGGAAACTCAACCTAGCGTCCTACCTCCCCCTGGGCCTCACACTGCATCCACTGGAGCCCCAGCTCTGG GCGGCCTATGGTGTGAACTCACACCGTGGACACCTGGGGACCAAGAATCTATGCGTGGAGGTGTCTGACC TAATCAGTATCCTGGTGCACGCCGAGGCCCAGCTGCCTCCCTGGTATCGAGCACAGAAAGATTTCCTCTC AGGCCTGGATGGGGAAGGACTCTGGTCTCCAGGGAGCCAGACCAGCACTGTGTGGCATGTGTTCCGGGCC CAGGATGCCCAGCGCATCCGTCGCTTTCTCCAGATGGTGTGCCCAGCTGGAGCAGGAACCTTGGAGCCTG GTGCCCCAGGCAGCTGCTACTTGGATGCAGGGTTGCGCCGACGGCTAAGAGAAGAGTGGGGTGTGAGCTG CTGGACCCTGCTGCAGGCTCCTGGGGAAGCGGTGCTGGTCCCGGCTGGGGCGCCCCATCAGGTGCAGGGC CTGGTGAGCACAATCAGTGTCACTCAGCACTTTCTGTCTCCTGAGACCTCTGCCCTCTCTGCTCAGCTCT ACCACCAGGGAGCCAGCCTACCCCCTGACCACCGTATGCTTTATGCCCAGATGGACCGGGCTGTGTTCCA AGCAGTAAAGGCGGCTGTGGGGGCGTTACAGGAAGCTAAATAGGGGATCCTGGTGCCTGGAGGGGTGGTG GGTAGGTAACCGAGTGTCCAGTCTGCATAATATCAACAGGGAATGAATGGTACTGGGGAACTTAGACGGT GTGGTCCGTTCTACAAATATCTCTTTGGGCACAAGCAGGGAACCTTGTTCCTCGTCCTGGCCCTGGCTCT AAAGGGAACAACTGTAATGCTTACAGCTGCCCCCCTCTCCATGGTATCAGGTTCATGCTAGGGGTATATG ATCCCTTCTAACCCCTGCACTCCACCCCAGGGGATGAACATCCTTCCTTGGGGAAGCTTGGGATTGTTTT GGTTTAAGCAACACTTCCCCTTGCTGCTTTATCCTGTGTTGCCCCTGGCACCCCACCCTCACCCCAGCTC ACACCAACATCCACACAAAGCCCTTCTGACTCTGTAGGTCTACTGGACCGGGAGCCACAGAATTCTTCCC TTATAGAAGAGCTGTGGAGTTGGGGAAGGGAGGGCCTCAGTTAATGGGCCAGACTGGCTGGAGGGAAGAA CATGGGCAGAACCACTGGGCCCTGCTTCTGCCTCTCTCCTCTCTCTCCTTCCCGGTCTCTATTAAAATCG GTTGTCTGGTGACTGCTGCCAGCAGTAGTTGGCCTTACTCTGCCAGCCACGTGGGCTAGGGAAAGAAGGC GAATGCAGAAATCTGATCTGGAGCAGAAGCCAGTGGACAGGGTGAGCAGGGACTGGGGACCTAGTGGCAG AGACCTCAAATACCACTGCCAGATCTTTGGGAGGCCTGGACCCCTGGAGCTTCCTGGGACTGAAGATATT CTTGACTGACAGCGGAAAGTGGAGTTCTAAGGTGCAGCACTCAGATGGCCTCCCTGGGTGGCATCAACCT GTGGAGATTACGGAACCCCCTGCCTCTGTCCCACCTATCCTGTGTGCTCTGACCTGGAGAGACGTCCAGG ATAGCCCTGGCTCATCCCAACTCCTCTTAGTGTCCAAGCCCCCAGGACAGAGCCTCACTGCCTCCGACAG CTGACCCCTCCCCCATGGCCCTTCTGGTGCCAACAGCTGACCCCTCCCCTCATGGTCCTTCTGGTGCCAA CAGCTGACCCCTCCCCCCATGGCCCTTCTGGTACTGCTTTTGCCTTCCTACCTCTTCCTCTGTGTATCTC AACTGCACACGTGCGCCAGCTCTTGTTATACTTAACAGTTCCTGCTGTGGTTTGGGGATTTTTTTTAATT AAATAAAAGTTTTTATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6468299 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6468299 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004031 same experiment
	EMAGE:30846 same embryo
	EMAGE:29661 same embryo
	EMAGE:29992 same embryo
	EMAGE:29658 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source