EMAGE mouse gene expression spatial database

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EMAGE:30118

1200011M11Rik ( MGI:1921383)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004906_01	euxassay_004906_02	euxassay_004906_03	euxassay_004906_04	euxassay_004906_05

euxassay_004906_06	euxassay_004906_07	euxassay_004906_08	euxassay_004906_09	euxassay_004906_10

euxassay_004906_11	euxassay_004906_12	euxassay_004906_13	euxassay_004906_14	euxassay_004906_15

euxassay_004906_16	euxassay_004906_17	euxassay_004906_18	euxassay_004906_19	euxassay_004906_20

euxassay_004906_21	euxassay_004906_22	euxassay_004906_23	euxassay_004906_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6926
Entity Detected:	1200011M11Rik, ( MGI:1921383)
Sequence:	sense strand is shown >T6926 GCTAGAGGAAGCTCTTTCTCCGCCATGGCGGGGCCGGTCAGCTTGCGCGAGCTGCTCATGGGGGCGTCGG CCTGGCTGGGCTCTGAGAGTCCTGGAGGCCCCTCCGCTGAAGGCGGGGGAAACGCGGCTGGCACGCCCGA GCCCCCGTGGAGGGAGGATGAGATCTGCGTGGTGGGAATCTTCGGCAAGACGGCTCTGCGCCTCAACTCC GAGAAGTTCTCTCTGGTGAATACTGTGTGCGATCGACAGGTTTTCCCACTCTTTCACCATCAGGATCCTG GAGATCCAGGAACTGGAATCAAGACCAAAGCTGTCTCTGTCGGAGAGGCGGGAGGAGCCGGAGACCCCGG GGCTGCAGCCGGGGATTCACTTCGCGGAGGTATGGCTACGGCGGAAGGTAACCGAGCTGAGCCAGGTCCC CAGGACTTCAGTCTTTTGCAGGCCTATTACAATCAGGAAAGCAAAGTTCTGTATCTTATTCTCACTTCCA TCTGTGACAACTCACAGCTTCTGCGTGCTTGTCGGGCCCTCCAGAGCGGTGAAGCCGGAGGGGGCCTCTC TTTACCTCATGCAGAAACACACGAGTTCTGGAAGCATCAGGAGAAGTTACAGTGTCTCAGCCTCCTTTAC CTCTTCTCTGTCTGTCATATCTTGCTCCTGGTCCATCCGACGTGTTCCTTTGACATCACTTATGATCGTG TATTCAGAGCCCTGGATGGACTGAGACAGAAAGTACTACCCCTCCTCAAAACAGCCATTAAGGATTGCCC AGTTGGCAAAGACTGGAAGTTAAACTGCCGACCTTGCCCACCTAGACTCCTCTTCCTCTTTCAACTCAAT GGGGCCCTTAAGGTGGAACCGCCTCGGAGCCAGGATACAGCTCATCCAGATAAACCCAAGAAGCACTCTC CCAAAAGAAGGCTGCAGCATGCCCTGGAGGACCAGATCTATAGAATCTTTCGCAAGAGTCGAGTCTTGAC TAATCAGAGTATCAACTGCCTCTTTACGGTACCTGCCAACCAAGCTTTTGTGTACATAGTCCCCGGAAGC CAGGAGGAGGACCCAATTGGCATGTTGCTGGACCAGCTTAGGAGCCATTGTACCGTGAAAGACCCAGAAT CGTTGTTGGTGCCCGCACCCCTTTCTGGACCTAGGCGGTACCAAGCGATGAGACAGCACAGCCGCCAACA GCTCTCCTTCCACATTGATAGCAGCACCTCGAGTTCTTCAGGCCAGCTAGTGGATTTCACTCTTCGAGAA TTCCTGTGGCAGCACGTGGAGCTGGTCCTAAGCAAAAAAGGTTTTGATGACAGTGTGGGCAGGAACCCAC AGCCTTCCCATTTTGAACTGCCCACTTACCAGAAGTGGATCTCTGCAGCAGCAAAGTTGTATGAAGTGGC TATCGATGGGAAGGAAGAGGACTTGGGCTCCCCCACTGGGGAGCTAACATCTAAGATTTTAAGCAGTATT AAAGTCTTAGAAGGGTTTTTGGATATAGACACAAAATTCTCGGAAAACCGGTGCCAAAAAGCTTTACCCA TGGCTCATAGTGCCTACCAGTCAAACTTGCCGCATAATTACACCATGACAGTCCATAAGAATCAGCTTGC TCAGGCTCTTCGGGTATACAGTCAGCATGCTCGAGGTCCAGCCTTTCACAAATATGCCATGCAATTACAC GAAGACTGCTACAAATTTTGGAGCAATGGCCATCAGCTGTGCGAAGAGAGGAGCTTGACTGACCAACACT GTGTCCACAAATTTCATTCCTTACCTAAATCAGGAGAAAAGCCAGAGGCAGATAGGAACCCTCCTGTGCT CTACCACAATAGCCGAGCCCGATCCACCGGAGCCTGCAACTGCGGAAGGAAGCAGGCACCTCGAGACGAC CCCTTCGATATCAAGGCTGCTAACTATGACTTTTACCAGCTTCTGGAAGAAAAATGTTGTGGGAAATTGG ATCATATCAATTTCCCTGTATTTGAGCCAAGTACTCCAGATCCCGCTCCTGCTAAAAATGAACCCTCTCC TGCCCCGCCAGACTCGGACGCCGAGAAACTGAAGGAGAAAGAACCTCAAACCCAAGGAGAGAGCACAAGC CTCAGCTTAGCTTTGAGCTTGGGCCAGTCAACAGATAGCCTAGGTACCTACCCCGCTGATCCACAGGCAG GAGGAGACAACCCAGAAGTCCACGGGCAAGGAGAAGGAAAGTCTGAGAAGAGACCAAACTTGGTTGACCG TCAGGCATCCACAGTTGAATATCTCCCAGGCATGCTACATTCAAATTGCCCCAAAGGTCTCCTACCCAAA TTCTCCAGCTGGTCTTTGGTTAAACTAGGCCCAGCCAAGTCTTACAACTTTCATACAGGTTTGGACCAAC AGGGCTTCGTTCCAGGAACAAACTATCTTATGCCTTGGGACATTGTCATCAGGACTAGAGCTGAAGATGA AGGAGACTTAGACACGAACTCCTGGCCTGCTCCAAATAAAGCTATTCCTGGGAAGAGAAGTGCAGTTGTG ATGGGCAGAGGAAGGCGGAGGGATGACATAGCTCGAGCCTTTGTTGGCTTTGAGTATGAAGACTCTCGAG GTCGGAGGTTCATGTGCTCAGGGCCTGACAAAGTAATGAAAGTGATGGGAAGTGGACCCAAGGAGTCGGC TTTAAAAGCCCTCAACAGTGACATGCCCCTGTACATCCTGTCCTCGTCGCAGGGCAGAGGGCTGAAGCCA CATTATGCTCAACTTATGAGGCTTTTTGTTGTGGTTCCTGATGCTCCTTTGCAGATAATACTGATGCCTC AGGTTCAACCAGGCCCACCACCATGTCCAGTATTTTACCCAGAAAAACAAGAAATCACTCTCCCACCTGA TGGCCTTTGGGTCTTGAGATTTCCTTATGCCTATGTGACTGAGAGAGGACCTTGTTTCCCTCCCAAGGAG AATGTGCAGCTCATGAGCTACAAGGTGCTCCGAGGAGTCCTCAAAGCAGTTACACAATGAGTCTTCCAGC CACTCTGCCGCAGACTGAAGCTGCTGTTTGTCTTGTTTTTGATGTGTTTGGGTTTTTTTTTAATGTGTAT ACTGTGTCTTCCCAAAACCCAATGTGTTTTGGTTACAAGTATTCAGTATTTGGACAATAATTGTTCCATT ATTTCATTATATTGATATTTATTGTAAAATCAATAAAAGTTTGTTCTAGAAAAAAAAGGATACAAAATGT AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3981459 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3981459 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004906 same experiment
	EMAGE:30956 same embryo
	EMAGE:30448 same embryo
	EMAGE:31157 same embryo
	EMAGE:30133 same embryo
	EMAGE:31019 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source