EMAGE mouse gene expression spatial database

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EMAGE:30133

Cul3 ( MGI:1347360)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004919_01	euxassay_004919_02	euxassay_004919_03	euxassay_004919_04	euxassay_004919_05

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euxassay_004919_11	euxassay_004919_12	euxassay_004919_13	euxassay_004919_14	euxassay_004919_15

euxassay_004919_16	euxassay_004919_17	euxassay_004919_18	euxassay_004919_19	euxassay_004919_20

euxassay_004919_21	euxassay_004919_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6925
Entity Detected:	Cul3, ( MGI:1347360)
Sequence:	sense strand is shown >T6925 CGGACGCGTGGGCGGACGCGTGGGCGCCGCCCCCCGCCGAGCCCCGCGGCTGCCCTCCCGCCTTCAATGT GACACCGGCGCCTCCGAGTGCGAGCCGCAGCCGCCGCCGCCGTCAGCGCCGCCGCCGGGGAGGGGCCGAG CACCATGTCGAATCTGAGCAAAGGCACGGGCAGCCGGAAGGACACCAAGATGCGGATCCGGGCCTTTCCG ATGACCATGGATGAAAAATATGTAAACAGCATTTGGGACCTTCTGAAAAATGCAATTCAAGAAATCCAGC GTAAGAATAACAGTGGTCTTAGTTTTGAGGAGCTCTATAGAAATGCATATACAATGGTTTTGCATAAACA TGGAGAAAAGCTCTACACTGGACTAAGAGAAGTTGTTACTGAACATCTCATAAATAAGGTGCGAGAAGAT GTACTAAATTCATTGAATAACAACTTTCTTCAAACACTAAATCAAGCTTGGAATGATCATCAAACAGCCA TGGTGATGATTAGAGACATATTAATGTATATGGACCGTGTATATGTACAACAGAATAATGTAGAAAATGT CTACAATTTGGGATTAATAATTTTTCGAGATCAAGTTGTACGGTATGGGTGTATTAGGGATCATCTTCGG CAAACTTTATTGGATATGATTGCCAGAGAGCGGAAAGGAGAAGTTGTAGACAGAGGAGCAATAAGAAATG CTTGCCAGATGTTAATGATTTTAGGTCTTGAAGGAAGATCAGTCTATGAGGAAGATTTTGAGGCTCCGTT TTTGGAAATGTCTGCAGAATTTTTTCAGATGGAAAGCCAGAAATTTTTAGCAGAAAACAGTGCTTCAGTA TATATAAAGAAAGTAGAAGCTAGGATTAATGAGGAAATAGAGCGGGTGATGCACTGCCTTGACAAATCCA CAGAAGAGCCTATTGTGAAGGTGGTGGAGAGGGAACTCATTTCCAAGCACATGAAGACTATTGTAGAAAT GGAAAATTCTGGGCTAGTACATATGCTGAAAAATGGAAAGACAGAAGACCTTGCTTGCATGTACAAATTA TTTAGTCGTGTGCCAAATGGTTTGAAGACCATGTGTGAATGTATGAGTTGTTATTTGAGGGAACAAGGAA AAGCCCTTGTTTCTGAGGAAGGAGAAGGGAAGAATCCTGTTGACTATATCCAGGGCTTATTGGATCTAAA GAGTAGGTTTGATCGCTTCCTCCAAGAATCCTTCAATAATGACCGGCTCTTTAAACAGACTATTGCTGGG GACTTCGAGTATTTCTTAAACCTCAACTCTAGGTCTCCTGAATACCTCTCATTATTTATTGATGATAAAC TGAAAAAGGGAGTCAAAGGGCTAACAGAACAAGAAGTAGAAACAATATTGGATAAGGCAATGGTCCTTTT TAGGTTTATGCAAGAAAAAGATGTATTTGAACGTTATTATAAACAACACCTGGCAAGAAGACTGCTCACA AATAAAAGTGTTTCTGATGACTCTGAGAAAAATATGATTTCTAAATTAAAGACTGAGTGTGGATGTCAAT TCACATCGAAACTGGAAGGAATGTTTAGGGATATGAGCATCTCAAACACAACTATGGATGAGTTCAGGCA ACATCTACAGGCAACGGGGGTCTCTTTAGGTGGTGTTGATCTCACAGTCCGGGTTCTCACAACGGGATAT TGGCCTACTCAGTCAGCCACACCAAAGTGCAACATCCCACCAGCACCAAGACATGCTTTTGAGATATTTA GAAGGTTCTACTTAGCCAAACACAGTGGTCGACAGCTCACACTCCAGCATCACATGGGTTCTGCAGATCT CAATGCCACCTTTTATGGTCCAGTTAAAAAGGAAGATGGATCTGAGGTTGGTGTTGGCGGTGCACAAGTA ACTGGTTCTAATACACGGAAGCACATACTTCAAGTCTCCACTTTCCAGATGACCATATTAATGCTTTTTA ATAATAGAGAGAAGTACACATTTGAGGAAATTCAACAAGAGACAGATATCCCTGAAAGAGAACTTGTTAG AGCCCTCCAGTCCCTCGCCTGTGGTAAACCAACACAGCGGGTCCTCACAAAAGAACCCAAGTCCAAGGAG ATAGAAAGTGGCCACATATTTACAGTTAATGATCAATTCACATCTAAACTACACAGAGTCAAGATTCAAA CAGTTGCAGCCAAACAAGGTGAATCCGACCCAGAAAGGAAAGAAACAAGACAGAAAGTAGATGATGACAG AAAACATGAGATAGAAGCTGCTATAGTGCGAATAATGAAGTCTAGGAAGAAGATGCAGCACAATGTTTTA GTAGCAGAGGTAACTCAGCAACTGAAGGCTCGATTCTTACCAAGTCCAGTTGTTATTAAGAAACGTATTG AAGGACTTATTGAGAGAGAATATTTGGCACGAACACCTGAGGATCGCAAAGTATACACATATGTAGCATA AAATGCATTCAGAAATTTGATTTATTCTTGGACTGTACTCTTCGCATGGACTGGGAAGTTCTTTTAAATC ATACTATTAAGACGACCATCTCTTCTGTTAAATTGCAGTACGTGTTATAGACCACTCAGATCAAGCCTCT ACTCCCTCTGAGAGTTTTCAACATCAGTTGATTGAGCTTCAGGCTTTCCAACGTTATCCCTGTAGAGATC ATCTTTACAGTTCCTCGGGAAAATGTGAATGTGCCGCGTTTTGTTTTCAATATTGTATGAAAACAGGAAA AAAATAAAACAAATTTAGAAAACACAGCTCATTAAAATAAAATTGTTGGATTTCATTTCAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:3981170 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3981170 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004919 same experiment
	EMAGE:30956 same embryo
	EMAGE:30118 same embryo
	EMAGE:30448 same embryo
	EMAGE:31157 same embryo
	EMAGE:31019 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source