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EMAGE:30140

Atp5o ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit ( MGI:106341)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50004
Entity Detected:Atp5o, ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit ( MGI:106341)
Sequence:sense strand is shown

>T50004
CGACAGGTTCGGAGCTTCAGTACATCTGTGGTCAGGCCCTTTGCCAAGCTTGTAAGGCCCCCTGTTCAGG
TCTACGGCATCGAAGGCCGCTATGCAACCGCCCTGTACTCTGCTGCATCTAAGGAGAAGAAGCTGGACCA
GGTGGAGAAGGAGCTGCTGCGCGTAGGGCAACTCCTGAAGGACCCCAAAGTGTCTCTGGCTGTTCTGAAT
CCCTACATCAAGCGCACCGTCAAAGTGAAAAGCCTAAATGACATCACGAAAAGGGAAAAGTTCTCCCCGC
TGACGGCCAACCTCATGAATTTACTTGCTGAAAATGGTCGCCTAGGCAACACCCAGGGTATCATCTCTGC
CTTTTCCACCATCATGAGTGTCCACCGCGGAGAAGTGCCATGCACAGTGACCACAGCATCTCCTCTAGAT
GACGCTGTTCTCTCTGAGTTAAAGACGGTGCTGAAGAGCTTCCTGAGTCCAAACCAAATACTGAAACTGG
AGATCAAGACTGACCCGTCAATCATGGGCGGGATGATTGTCCGAATTGGGGAAAAGTACGTTGATATGTC
TGCAAAGAGCAAGATTCAGAAGCTCAGCAAGGCCATGCGGGAGATGCTCTGAGAGACTGTCACCTGTGTG
AGCTCTTGTCCTTGGAGCAACAATAAAATGCTTCCTG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers.. The reverse primer contains a 5' extension containing . T7 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template usi. T7 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_007188 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS