EMAGE mouse gene expression spatial database

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EMAGE:30167

Map3k4 mitogen-activated protein kinase kinase kinase 4 ( MGI:1346875)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006299_01	euxassay_006299_02	euxassay_006299_03	euxassay_006299_04	euxassay_006299_05

euxassay_006299_06	euxassay_006299_07	euxassay_006299_08	euxassay_006299_09	euxassay_006299_10

euxassay_006299_11	euxassay_006299_12	euxassay_006299_13	euxassay_006299_14	euxassay_006299_15

euxassay_006299_16	euxassay_006299_17	euxassay_006299_18	euxassay_006299_19	euxassay_006299_20

euxassay_006299_21	euxassay_006299_22	euxassay_006299_23	euxassay_006299_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8508
Entity Detected:	Map3k4, mitogen-activated protein kinase kinase kinase 4 ( MGI:1346875)
Sequence:	sense strand is shown >T8508 CCCGCCGCGGTCATGCGAAGCTTGGTGCACGGATGAGAGACGCCATCGCCGAGCCGGTGCCCCCTCCTGC CCTCGCCGACACCCCTGCAGCCGCCATGGAGGAGCTGCGGCCAGCACCGCCGCCACAGCCCGAGCCGGAT CCGGAGTGCTGCCCAGCGGCGAGGCAGGAGTGCATGTTGGGAGAGTCGGCTCGCAAAAGTATGGAATCCG ATCCAGAGGACTTTTCTGATGAAACAAATACAGAGACTCTCTACGGCACCTCACCCCCAAGCACACCTCG ACAGATGAAACGCCTGTCAGCCAAGCACCAGAGGAACAGCGCAGGGAGGCCGGCCAGCCGATCGAACTTG AAAGAAAAAATGAACACACCGAGTCAGTCTCCACATAAAGATTTGGGGAAGGGAGTGGAGACCGTGGAAG AATACAGCTACAAGCAGGAGAAGAAGATTCGAGCAACTCTGAGAACAACGGAGCGAGACCATAAGAAAAA TGCACAGTGCTCGTTCATGTTGGACTCGGTGGCTGGGTCTTTGCCAAAAAAATCGATTCCAGATGTGGAT CTCAATAAGCCTTACCTCAGTCTCGGCTGTAGCAATGCCAAGCTGCCCGTCTCGATGCCCATGCCGATAG CCAGAACTGCACGGCAGACTTCCCGGACTGACTGCCCCGCAGATCGCTTAAAGTTCTTTGAAACACTGCG CCTTTTGCTAAAGCTTACCTCAGTCTCGAAGAAGAAGGACAGGGAGCAGAGGGGACAAGAAAACACGGCT GCTTTCTGGTTCAACCGATCGAACGAACTGATCTGGTTAGAACTGCAGGCCTGGCACGCGGGCCGCACCA TCAATGACCAGGACCTCTTTCTCTACACAGCCCGCCAGGCCATCCCAGACATCATCAATGAGATCCTCAC CTTCAAAGTTAACTACGGGAGCATTGCCTTCTCCAGCAATGGAGCCGGTTTCAACGGGCCCTTGGTAGAA GGCCAGTGCAGAACCCCTCAGGAGACAAACCGTGTGGGCTGCTCATCGTACCACGAGCACCTCCAGCGCC AGAGGGTCTCGTTTGAGCAGGTGAAGCGGATAATGGAGCTGCTGGAGTACATGGAGGCACTTTACCCATC CTTGCAGGCTCTGCAGAAGGACTATGAACGGTACGCCGCCAAGGACTTTGAGGACAGAGTGCAGGCGCTC TGCCTGTGGCTCAACATCACGAAAGATCTAAATCAGAAGCTGCGGATCATGGGCACCGTGCTGGGCATCA AGAACCTATCAGACATTGGCTGGCCAGTGTTTGAAATCCCCTCCCCTCGGCCGTCCAAGGGCTACGAGCC AGAGGACGAGGTCGAGGACACGGAGGTTGAGCTGAGGGAGCTGGAGAGCGGGACGGAGGAGAGTGACGAG GAGCCAACCCCCAGTCCGAGGGTGCCAGAGCTCAGGCTGTCCACAGACGCCATCTTGGACAGTCGCTCCC AGGGCTGCGTCTCCAGGAAGCTGGAGAGGCTCGAGTCAGAGGAAGATTCCATAGGCTGGGGGACAGCGGA CTGTGGCCCTGAAGCCAGCAGGCATTGTTTGACTTCTATCTATAGACCATTCGTGGACAAAGCACTGAAG CAAATGGGGCTAAGAAAGTTAATTTTACGACTTCATAAGCTTATGAATGGGTCCTTGCAAAGAGCTCGTG TAGCTCTGGTGAAGGACGACCGTCCAGTGGAGTTCTCTGACTTTCCAGGTCCCATGTGGGGCTCGGATTA TGTGCAGTTGTCGGGAACACCTCCTTCCTCAGAGCAGAAGTGTAGCGCTGTGTCCTGGGAAGAACTGAGA GCCATGGACCTGCCTTCCTTTGAGCCCGCCTTCCTGGTGCTCTGTCGGGTCCTGCTGAACGTGATCCACG AGTGCCTGAAGCTGCGGCTGGAACAGAGGCCTGCCGGGGAGCCTTCCCTCTTGAGTATCAAACAGCTAGT GCGAGAGTGTAAAGAGGTCCTAAAGGGCGGGCTCCTGATGAAGCAGTATTACCAGTTCATGCTGCAGGAG GTCCTGGGCGGACTGGAGAAGACCGACTGCAACATGGATGCCTTTGAGGAGGACCTGCAGAAGATGCTGA TGGTGTATTTTGATTACATGAGAAGCTGGATCCAAATGCTACAGCAGTTACCTCAGGCTTCCCATAGCTT AAAAAACCTGCTAGAAGAGGAATGGAATTTCACCAAAGAAATAACCCATTATATCCGTGGCGGAGAAGCG CAGGCTGGAAAGCTTTTCTGTGACATCGCAGGGATGCTGCTGAAATCCACAGGGAGCTTTCTGGAATCCG GCCTGCAGGAGAGCTGTGCTGAGCTGTGGACCAGCGCCGACGACAACGGTGCTGCCGACGAGCTAAGGAG ATCTGTCATCGAGATCAGCCGAGCACTCAAGGAGCTCTTCCACGAAGCCAGGGAAAGAGCCTCCAAGGCC CTGGGCTTTGCTAAAATGCTGAGGAAGGACCTAGAAATAGCAGCAGAGTTCGTGCTATCTGCATCAGCCC GAGAGCTCCTGGACGCTCTGAAAGCAAAGCAGTATGTTAAGGTACAGATTCCCGGGTTAGAGAATTTGCA CGTGTTTGTCCCCGACAGCCTCGCTGAGGAGAAGAAAATTATTTTGCAGCTACTCAATGCTGCCACAGGA AAGGACTGCTCAAAGGATCCAGACGACGTCTTCATGGATGCCTTCCTGCTCCTGACCAAGCATGGGGACC GAGCCCGTGACTCAGAAGATGGCTGGGGCACATGGGAAGCTCGGGCTGTCAAAATTGTGCCTCAGGTGGA GACTGTGGACACCCTGAGAAGCATGCAGGTGGACAACCTTCTGCTGGTTGTCATGGAGTCTGCTCACCTC GTACTTCAGAGAAAAGCCTTCCAGCAGTCCATTGAGGGGCTGATGACTGTACGCCATGAGCAGACATCTA GCCAGCCCATCATCGCCAAAGGTTTGCAGCAGCTCAAGAACGATGCACTTGAGCTATGCAACAGAATCAG CGATGCCATCGACCGTGTGGACCACATGTTCACCCTGGAGTTCGATGCTGAGGTCGAGGAGTCTGAGTCG GCCACGCTGCAGCAGTACTACCGAGAAGCCATGATTCAGGGCTACAACTTTGGGTTTGAGTATCATAAAG AAGTTGTTCGTTTGATGTCTGGGGAATTCAGGCAGAAGATAGGAGACAAATATATAAGCTTCGCCCAGAA GTGGATGAATTACGTGCTGACCAAATGCGAGAGCGGCAGAGGCACAAGACCCAGATGGGCCACCCAAGGA TTTGATTTCCTACAAGCCATTGAACCTGCCTTTATTTCAGCTTTACCAGAAGATGACTTCTTGAGTTTGC AAGCCCTGATGAATGAGTGCATCGGGCACGTCATAGGAAAGCCACACAGCCCTGTCACAGCTATCCATCG GAACAGCCCCCGCCCTGTGAAGGTGCCCCGATGCCACAGTGACCCTCCTAACCCTCACCTCATCATCCCG ACTCCAGAGGGATTCAGCACCCGGAGCGTGCCTTCCGACGCTCGGACCCATGGCAACTCTGTTGCTGCTG CTGCTGCTGTTGCTGCCGCCGCCACCACTGCTGCTGGCCGCCCTGGCCCAGGTGGTGGTGACTCTGTGCC AGCCAAACCTGTCAACACTGCCCCTGATACCAGGGGTTCCAGTGTCCCTGAAAACGACCGCTTGGCCTCC ATAGCTGCAGAACTGCAGTTCAGGTCTCTGAGTCGGCACTCAAGCCCCACGGAAGAGCGAGACGAGCCAG CGTATCCTCGGAGTGACTCAAGTGGATCAACTCGGAGAAGCTGGGAACTTCGAACACTCATCAGCCAGAC CAAAGACTCGGCCTCTAAGCAGGGGCCCATAGAAGCTATCCAGAAGTCAGTCCGACTGTTTGAAGAGAGG AGGTATCGAGAGATGAGGAGAAAGAATATCATCGGCCAAGTGTGCGATACCCCTAAGTCCTATGATAACG TCATGCATGTTGGACTGAGGAAGGTGACATTTAAGTGGCAAAGAGGAAACAAAATTGGAGAAGGACAGTA TGGAAAAGTATACACCTGCATCAGTGTTGACACAGGGGAGCTGATGGCCATGAAGGAGATTCGATTTCAG CCTAACGACCACAAGACTATCAAGGAGACTGCAGACGAGTTGAAAATATTTGAAGGCATCAAGCACCCCA ACCTGGTCCGGTATTTTGGCGTGGAGCTTCACAGGGAAGAGATGTACATCTTCATGGAGTACTGTGATGA GGGTACACTAGAGGAGGTGTCACGACTGGGCCTGCAGGAGCACGTCATCAGGTTATATACCAAGCAGATC ACTGTCGCCATCAACGTCCTCCATGAGCACGGCATCGTTCACCGAGACATCAAAGGTGCCAATATCTTCC TTACGTCATCTGGACTAATCAAGCTGGGAGATTTTGGATGCTCTGTAAAACTTAAAAACAACGCCCAGAC CATGCCCGGAGAGGTGAACAGCACCCTAGGGACAGCAGCTTACATGGCCCCTGAAGTTATTACCCGAGCC AAAGGAGAAGGCCACGGACGTGCGGCAGATATCTGGAGTCTGGGGTGCGTCGTCATAGAGATGGTGACTG GCAAGCGGCCTTGGCATGAGTATGAACACAACTTTCAGATTATGTACAAGGTGGGGATGGGACACAAGCC ACCAATCCCGGAAAGGCTAAGCCCTGAAGGAAAGGCCTTTCTCTCGCACTGCCTGGAAAGTGACCCGAAG ATACGGTGGACAGCCAGCCAGCTCCTCGACCACGCTTTTGTCAAGGTTTGCACAGATGAAGAGTGAAGTG AACCAGTCCGTGGCCTAGTAGTGTGTGGACAGAATCCCGTGATCACTACTGTATGTAATATTTACATAAA GACTGCAGCGCAGGCGGCCTTCCTAACCTCCCAGGACTGAAGACTACAGGGGTGACAAGCCTCACTTCTG CTGCTCCTGTCGCCTGCTGAGTGACAGTGCTGAGGTTAAAGGAGCCGCACGTTAAGTGCCATTACTACTG TACACGGGCCACCGCCTCTGTCCCCTCCGACCCTCTCGTGACTGAGAACCAACCGTGTCATCAGCACAGT GTTTTTGAGCTCCTGGGGTTCAGAAGAACATGTAGTGTTCCCGGGTGTCCGGGACGTTTATTTCAACCTC CTGGTCGTTGGCTCTGACTGTGGAGCCTCCTTGTTCGAAAGCTGCAGGTTTGTTATGCAAGGCTCGTAAG TGAAGCTGAAGAAAAGGTTCTTTTTCAATAAATGGTTTATTTTAGGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5705378 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5705378 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006299 same experiment
	EMAGE:30157 same embryo
	EMAGE:30484 same embryo
	EMAGE:31634 same embryo
	EMAGE:31601 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source