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EMAGE:302

Wnt4 wingless-related MMTV integration site 4 ( MGI:98957)
TS24 (16.5 dpc)
in situ hybridisation

Data Images
EMAGE:302
Figure 4C of McCright et al., 2001 [PMID:11171333] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
Not specified by authors what arrows indicate.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
renal cortex
detected detected
regionalExpression in epithelial vesicles and comma shape bodies.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2149947
Entity Detected:Wnt4, wingless-related MMTV integration site 4 ( MGI:98957)
Notes:The Wnt4 probe used in this study by McCright et al., 2001 [PMID:11171333] is indicated by the authors as that used by Stark et al., 1994 [PMID:7990960] which was originally described by Parr et al., 1993 [PMID:8275860] as a "420bp SmaI-SmaI (coding and 3' untranslated) " fragment. Editors Note: Additional details about the probe used in this assay were provided by the paper's first author to GXD/MGI staff (see MGI:2149947).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6J
Age:16.5 dpc
Theiler Stage:TS24
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
General Information
Authors:McCright et al., 2001 [PMID:11171333] Indexed by GXD.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11171333] McCright B, Gao X, Shen L, Lozier J, Lan Y, Maguire M, Herzlinger D, Weinmaster G, Jiang R, Gridley T 2001 Defects in development of the kidney, heart and eye vasculature in mice homozygous for a hypomorphic Notch2 mutation. Development (128):491-502
 [ doi:10.1038/372679a0] [ PMID:7990960] Stark K, Vainio S, Vassileva G, McMahon AP 1994 Epithelial transformation of metanephric mesenchyme in the developing kidney regulated by Wnt-4. Nature (372):679-83
 [ PMID:8275860] Parr BA, Shea MJ, Vassileva G, McMahon AP 1993 Mouse Wnt genes exhibit discrete domains of expression in the early embryonic CNS and limb buds. Development (119):247-61
Links:MGI:2150120 same experiment
 MGI:2149947 probe description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI