EMAGE mouse gene expression spatial database

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EMAGE:30246

Txnip thioredoxin interacting protein ( MGI:1889549)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004871_01	euxassay_004871_02	euxassay_004871_03	euxassay_004871_04	euxassay_004871_05

euxassay_004871_06	euxassay_004871_07	euxassay_004871_08	euxassay_004871_09	euxassay_004871_10

euxassay_004871_11	euxassay_004871_12	euxassay_004871_13	euxassay_004871_14	euxassay_004871_15

euxassay_004871_16	euxassay_004871_17	euxassay_004871_19	euxassay_004871_20	euxassay_004871_21

euxassay_004871_22	euxassay_004871_23	euxassay_004871_24	euxassay_004871_25	euxassay_004871_26

euxassay_004871_27	euxassay_004871_28

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6534
Entity Detected:	Txnip, thioredoxin interacting protein ( MGI:1889549)
Sequence:	sense strand is shown >T6534 GGGATTTCCAGAGTTTCTCCAGTTGCGGAAGACAGCTGTTATTTTTCTCCTGAAAGCTCTTGGCACAGCC GGCAGGTTGAAACTTTCAGGCACCTTTTGGAAAAGTTGTTAGGGTTTGTTTGAAGCTTTCTTTACATTTT CGTTTGGGTTTTCAAGCCCTGACTTTACGGAGGTGAGCCCTTCGTTTGCTTTGAAGGGTTCTTAAAGATT TTTTTCCTCTCCGGCTTTCGTTTTTCTTGAACCCACTCGGCTCAATCATGGTGATGTTCAAGAAGATCAA GTCTTTTGAGGTGGTCTTCAACGACCCCGAGAAGGTGTACGGCAGCGGGGAGAAGGTGGCCGGACGGGTG ATAGTGGAAGTGTGTGAAGTTACCCGAGTCAAAGCCGTCAGGATCCTGGCTTGCGGCGTGGCCAAGGTCC TGTGGATGCAAGGGTCTCAGCAGTGCAAACAGACTTTGGACTACTTGCGCTATGAAGACACACTTCTCCT AGAAGAGCAGCCTACAGGTGAGAACGAGATGGTGATCATGAGGCCTGGAAACAAATATGAGTACAAGTTC GGCTTCGAGCTTCCTCAAGGGCCCCTGGGAACATCCTTTAAAGGAAAATATGGTTGCGTAGACTACTGGG TGAAGGCTTTTCTCGATCGCCCCAGCCAGCCAACTCAAGAGGCAAAGAAAAACTTCGAAGTGATGGATCT AGTGGATGTCAATACCCCTGACTTAATGGCACCAGTGTCTGCCAAAAAGGAGAAGAAAGTTTCCTGCATG TTCATTCCTGATGGACGTGTGTCAGTCTCTGCTCGAATTGACAGAAAAGGATTCTGTGAAGGTGATGACA TCTCCATCCATGCCGACTTTGAGAACACGTGTTCCCGAATCGTGGTCCCCAAAGCGGCTATTGTGGCCCG ACACACTTACCTTGCCAATGGCCAGACCAAAGTGTTCACTCAGAAGCTGTCCTCGGTCAGAGGCAATCAC ATTATCTCAGGGACTTGCGCATCGTGGCGTGGCAAGAGCCTCAGAGTGCAGAAGATCAGACCATCCATCC TGGGCTGCAACATCCTCAAAGTCGAATACTCCTTGCTGATCTACGTCAGTGTCCCTGGCTCCAAGAAAGT CATCCTTGATCTGCCCCTAGTGATTGGCAGCAGGTCGGGTCTGAGCAGCCGGACATCCAGCATGGCCAGC CGGACGAGCTCTGAGATGAGCTGGATAGACCTAAACATCCCAGATACCCCAGAAGCTCCTCCTTGCTATA TGGACATCATTCCTGAAGATCACAGACTAGAGAGCCCCACCACCCCTCTGCTGGACGATGTGGACGACTC TCAAGACAGCCCTATCTTTATGTACGCCCCTGAGTTCCAGTTCATGCCCCCACCCACTTACACTGAGGTG GATCCGTGCGTCCTTAACAACAACAACAACAACAACAACAACGTGCAGTGAGCCTGCAGGAAATGAAGCA TCTGTATTAGCGCATTTCTTTCTGCCTCTCTGCTTGAACTCCAGTGTTTCAGAGACTCAGTCTCTACAGC GGGGAACGGGTACACCCCAGCCGCTGACTCCTCAAGATGGGTGGCAATCAGTAGGCGGATCTCCGGCTTC AAGTGGTGCAGACCAGTGCCCGCACTGTGGCATAGGAGTGTTTGCTGGGTGGATGTCAGAACACTCTAGA AAAATTGAGACCTGACCACTTTCTCGGATGTTGGAAATGAAGAACTTGTTTGTGTTGACTGAGTCAGGGC ACTGCTGACCTTCTGGCGTTGTCTTTCCAAGGTTTTTGTTTTAAAGGGAATTTTAAATTGTCTAAAATAT CAGTAGACCATCATCTGTGCCATGGGGGACAGAGCCAATTTCAAGTCATGGCCAAAATTTTGTAAGAGGA GTGTTTTTGTGTGTTTTTTAAAGTCAGTGTTCCTTTTTTATATCTTTACAAAGAAAAGACCTTCCACGGC TGGTGAGCACGCAGCCTGTGAAATTCGGGGCAGCTGCTCCAAGTTGACTTCACCCTGGGAGCAGTAGTAG CTGTGCCCACTGACGGCCATAAAAGCCATTTTACAGCCAGTTGCACTGTGTTCTCTTGTAAGCATAATCA GATGGGAGAATCTGTTATTTCCCTGTAACCCCTTGGAATTGATTCTAAGGTGATGTTCTTAGCACTTTAG CTTGTCAGTTTTGTTTTAGTCTCCGTTATAGATGTAAGCTCCACCAGTCTCTTAAGGGTTAAGCCCAGTG ACTTGGAGGGTGGGGGTTAGGGTCTCTATCCCTGAACATTGTAGACCCAGGCTGGCCTGAGAGATCCACC TGCCTCTGCCTCCTGAGTGCTGCGATCAAAGGCCCAGCTTGGTTATTGCTTTTGAGGCTTTCTCCCAACG CACAGACTTGTGTAATTCTAACACTAACCCTGTGAAGGGTTGTGGTTGACAGCTGGAGCCTGGGTGACAT TCTACATTGAGATGCCCCAGCACTGATCGGGGCACAGAAGCCCCCAGACCCCATTTCCTGTCCAGTGTTG GGAGAAAGTGCTGCTTTCACTGTGGCCTCAGCCCTGGCTCGGAAGTTCACTAAGCCTTAGCACTTTGTCC TGTGTCAGCTCCACCTGAGAACTGTGCAGCCAGAATGTCTGCGAGCTGATGGAGGTTTCGGTTTTGTTGT TTTTGTATTTTGTGTATCTTTTTGTATGATTAAAAACTATATTTTCTACTTATCCAAATATATTTTCACC CCAAAGTGGGGTTATCCTTTGTAAAAAAAAAATAAAGTTTTTTAATGACAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3591421 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3591421 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004871 same experiment
	EMAGE:30269 same embryo
	EMAGE:30214 same embryo
	EMAGE:31028 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source