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EMAGE:30302

2610002M06Rik RIKEN cDNA 2610002M06 gene ( MGI:1914278)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4133
Entity Detected:2610002M06Rik, RIKEN cDNA 2610002M06 gene ( MGI:1914278)
Sequence:sense strand is shown

>T4133
CTCGAAACCCGCTACAACCGCCACCCTCCGCGCGCCCAAGCGACCCACAACTCGCGGGTCCAGCCCCTGT
CCCGCGTGTTACCTGCCCCTCCCCCTCCTCCCGCCCGGTGAACTCGAGAGATCATGTCGAACATGGAGAA
ACACTTATTTAACTTGAAGTTTGCTGCAAAAGAACTTAATAGGAATGCCAAGAAGTGTGATAAAGAAGAA
AAAGCTGAAAAGGCCAAGATAAAAAAGGCCATTCAGAAGGGCAATACAGAAGTTGCAAGAATACATGCTG
AAAATGCAATCCGTCAGAAAAACCAAGCAATCAATTTCTTGAGAATGAGTGCTAGGGTTGATGCTGTGGC
AGCCAGAGTTCAAACTGCAGTAACAATGGGCAAGGTGACAAAATCCATGGCAGGTGTAGTTAAATCTATG
GATGCTACATTGAGGAGTATGAACCTTGAGAAGATTTCTGCCTTAATGGACAAATTTGAACATCAGTTTG
AAACATTGGATGTTCAGACACAGCAAATGGAAGATACAATGAGTAGCACTACTACCCTTACGACACCACA
GAACCAAGTGGATATGCTGCTCCAGGAAATGGCAGATGAAGCTGGCCTCGATCTCAATATGGAACTACCA
CAGGGTCAGACAGGGTCTGTTGGTGCAAGTGTTGCTTCTACAGAGCAGGATGAACTGTCACAGAGGCTTG
CTCGTCTTCGTGATCAAGTGTAACTTAAATAACTGGTGCTTCTGTTCCTCCACATATTTCTGAGCCATCA
TTTGTGTGTGTGTCTATAGATAAGGTGTATTCCAGTTACAAAAACATCATACATGACAGGATGTTTGGAC
ATCATTTCTATAAGATGCTTTCTTTTGCCTTGTGTATTTCACAGTGCATTGCGAAATTCCAAGGAATTTC
CACATTGGCAGATTTTATAGTTCTATATATTGTGTGTAAAAGTGTGAGGGACATATTTTTCTAGATGTCT
TTGGCAAAGCTAGCATTAGTTTTCTGTATATTATACATCTTCCGCAAATATTAGAGCAAACATCTAGCAC
CAAAGTAATGCTAAGTAAAGTAATTTTGTCAGAATAATGTATAAAGGCTTTGTTTTTACTTATTGAAAGT
AACTTTCAATTATTGTCTGTGATGAATTTTGATTTTGTAAAAATAAAAACAGGAAAGCATGTTCATTTAA
TATGTTCGCATAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4481241 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4481241 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_003986 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS