EMAGE mouse gene expression spatial database

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EMAGE:30324

2010011I20Rik RIKEN cDNA 2010011I20 gene ( MGI:1914267)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004003_01	euxassay_004003_02	euxassay_004003_03	euxassay_004003_04	euxassay_004003_05

euxassay_004003_06	euxassay_004003_07	euxassay_004003_08	euxassay_004003_09	euxassay_004003_10

euxassay_004003_11	euxassay_004003_12	euxassay_004003_13	euxassay_004003_14	euxassay_004003_15

euxassay_004003_16	euxassay_004003_17	euxassay_004003_18	euxassay_004003_19	euxassay_004003_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4427
Entity Detected:	2010011I20Rik, RIKEN cDNA 2010011I20 gene ( MGI:1914267)
Sequence:	sense strand is shown >T4427 CCCACGCGTCCGAGCTGGTTCCCGAGTGGGCACACTGGAGCTGCGCTCGCGGCGCGCCATGGCCGGGCTG TTGACGCTGCTTGGCCCGGCAGGAAGAGTGAGCACCCGGCTGCGGCCCCTGGCCCCTTGGCTCCTGGGAA CCGCCACCTCCTGTGCTCCACCGCTCTGGGCCTTAGCCTTGTCCCATCCGGTTCCGGACGCCCGACTGCT GCGCACGGCCCGCGGGGACTGTCTCAGCCGCCAGGAGCCCAACAGGACCCCCGAGCCAGGAGGCGGTGTC ACCGGCACAGAGAAAAAGCTAAGCAGAACACAGCAGCTGAAGAAGGTTTTCCAAGAGTACGGGGCCGTGG GCGTGTCAATGCACATTGGGATCTCCCTGGTCTCCTTGGGAATATTTTACACAGTTGTTTCCAGTGGCAT AGACATGTCTGCAATCCTGCTTAAACTTGGCTTTAAAGAGTCCCTGGTGCAGTCCAAAATGGCAGCAGGC ACCAGCACGTTCGTGGTGGCCTATGCCATTCATAAGCTGTTTGCTCCCGTTCGGATCAGCATCACCTTGG TCTCTGTGCCCTTTGTCGTCAGATACTTCCGCAGCGTTGGACTCTTTAAACCTCCAGCCACCAAGCCTTG ACGCCAGTCTCTTCTGATCTGCAAGATTTGGGCTGTGTGCAAGGTGGCTTTCTTTCCTGGTGGGGGAAGG GAGGGGCCGAGGCTAATTGCTACGTTCCCCGTGGGTTGGTTTTACTTTTATCATCAAACATCTGGTGTTT TAATTTACTTTTTTTTTTTAATGCTGTTCCTCATGTACATGACTTAAAAATGTCACCAAGGCATCAAAAT GAGTTGACAGCCAGGCCTGGTGGTACAGGCCAGTAAAGCCAGCTGCTCTGGAGGCGGAGGCAGGAGGATC TCAAATTCAAGCCTGCTTAAGCTACTTATAGTTCAGGGTCCGCTTAACAATTTATAAGACTGTGTCTTGT TCACCAGTGAGAGAGGGCTGAGGTCATTACTCACCTGGCACAGACGGCCTAAATGCAATCCCCAGCACAA AAAGAGACTGCCTACAAATTGTTCCTCTAGATCATCGGGGCAGAGGTGCTGTCTATAAAGCCCCCCGGGG GCTAACCTTTGCTCAGATTCTTAAAAAGCAGTGGTTAATTTGCTGGCTTTTCAAACCCTGCCCGCCCTGG TTTGCCTGCGGTTTAATATGCAATATTCAGCAGACAGCTGCTGGCGCTACAGTTAGTGAGGCGAAAATAA CCATGATCCACATTACCATCAAGCCCTTGACATTCGCAGCACTTGCTGTGCGGAAAGCAGGCCAAGAACT TGTCATGTGCCCCATCCACTTCGAGGCTGGATGGGATGGGGCTCCAAGTCTGAGATTTCATTGATACTGG GCAATATGGTTTGTAAAATACAGAAACACTGCCCAATTGTGATGTTGTCAATGGGCTCCTGATGCCCATA TTCTGGGGAACCTTAGATACTGCCATAGAAGCTAAAAGCACCCTAGCTTTCTTAGACAATCCACTTAGCT ATCTGAAACTTGAGGCATTCCAGACCTGACATGTTTACAATGTACCACTGAACTTTTTTAAATTGAATTA ATCAGATGTATATTTGTACCACTTTGGGGGTGGGGGGAGGTTGAGAGGAAAATATTGCTCGTTCTAACTG TAAAGAAACCAAGAAATAATACAAGCTGGGGAAAAGAGATGTCTTAATTAGGGTTCTATTGCTGTGATGA AACAAAATGACCAAACTCACGGTTCCATACAACAGTTCATCAAACACAGGGAGGGCAGGCACCTGGAGGC AGGAGCTGACAGCAGAGGCCAGAGAGGAGAGCCACTTACTGGCTTGTTCAGCTGCTTTCTTATAGAACAT AGCCCGCCAGCTCATGGGTGCCAGTGGTTCTCAACTTGTGAGTTGCAACTCCTTTGCCAAACCTCTAGCT CAACTCAAAACAGTAGCAAAATTATGAAGTAGCAATGAAAATAACCTTCTGGTTGGGGGCCACCATGAGG AACTGTACTAAAGGGTCGCAGCATGAGGAAGGTTGGGTACTGCTGGTGTAGAGGGAGGTTGGAATAGAAA GTCAAGCAGAGACTCAAACTGAGACTTCCTCCTCCACGAATTCAGGAGCAGACAGAGCAAGAATTGGCTC CACCGCTCAAGCCTGCCCTCTGGGCAGGCTGACGTGGGGACACTTGTTCTGTTCTGTCATATCCAAAACA AACTCCATCCCCCCCAAGGCAGCCCAAATATCCAGAAACGGGCTCACCTGGACTATAGAATTTCTGCTGG TCAGATAAAGACAGCATTCCTCAGGAACCCGTGAAACTGGTTCTCTCTCTACCAAATGGAGTCATTCTCC TTACTTCTGGCAGAAGGGTCATAGCTCTCCCAGTAATAGACCTGTGGTACCTAACGGCGTATCAGGGCAT GCTGGTATCACAAGACCCTGATACACATTTCAGCGTTCCTAGCCCTTCTTGTTCCCCCTACCCTAAACTG CCCCCAACTCACAGGCTTCCCGTCCCCCAGCTGCCTGTCCTTATAACCAGCTATTTTGTCCTCTTGGCTA CTGTTTCTCTCTGTGCTCTATTCTCTATCCTGTGCCAGTTTCCTCATCCCCTGGCCATGTTCAGTCCACC CCTCTCTCTCTCTCAACTTCAGATGCCTCTGGCTGTTCTCATGTCTATAATAAGAACCTCCCCACAACCA TACGAAGTATAACCATACGTCTATAGTTCTCCCCCGGGGAGCTAGTGTTTGAACTTTGAAGTCTGCTCAG CTGCATGGTGGAAAGGACTGTGTTCTTTTTTTTCTTGCTCACAATTCCTTTGAAATTACATTGACAGCCA GGAGACAAAGGAGAGAACTGTTAACTAGCAGAATGCATTTTATAGAGCTCTGGCTAACCGCCTCTTAGCC CACGGTTACCCAGTCATTTGTCTCAGTTAACATTTAAAGGAGTTTTTGTGAGACAAATTCTCCATTCTGA GATCTCTGAAGTGGGATTGTCTTATTTTCCTTAGTACAGCCACCACTGCCAAAAAGCTTCCTCTGATGTC AAATCAGGATGTATTACCGAAACTGATTCCAAGGACATCCACATAGCTGAGCCAAGGTGCCCCAGGCCAG TGGCAGGCGGACTGGAAGGGGCCTCTCCACCGAGCCTGTGTTCTAACAGCTTCAGGCTCTGGATCATCAC CCCCGCTGCTCTCCCATTCCGGTACCTAAGTAACATCTATGCAAAGAAACACATTAAAAGCAGCAATAAA ACTGCTCCTACCCACAGCCACGGATCTTCCAAACCTAGCCCCGCTCTCAGGCCTCTACCCAGCAGCCAGA ACACAGCAAGAGTTGAGGTGAAGCAACTAAAGCACCGTATAAGCCAGCAAGGCGCACAGAGCTGCTCCTG CCTATGTGATCTTTAAGTAGACCAGGAGCCAGGAAACCACTTTAAGATGTAAGCCATTGTGTGTCTCTAG TTTCTTAAGCAGACCAAGTGAATGGCTTACAGTCTGAAACAAATAAAAGCCTTTGTAAAAGTCAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4924329 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4924329 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004003 same experiment
	EMAGE:30327 same embryo
	EMAGE:30325 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source