EMAGE mouse gene expression spatial database

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EMAGE:30331

Stxbp1 syntaxin binding protein 1 ( MGI:107363)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004017_01	euxassay_004017_02	euxassay_004017_03	euxassay_004017_04	euxassay_004017_05

euxassay_004017_06	euxassay_004017_07	euxassay_004017_08	euxassay_004017_09	euxassay_004017_10

euxassay_004017_11	euxassay_004017_12	euxassay_004017_13	euxassay_004017_14	euxassay_004017_15

euxassay_004017_16	euxassay_004017_17	euxassay_004017_18	euxassay_004017_19	euxassay_004017_20

euxassay_004017_21	euxassay_004017_22	euxassay_004017_23	euxassay_004017_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4131
Entity Detected:	Stxbp1, syntaxin binding protein 1 ( MGI:107363)
Sequence:	sense strand is shown >T4131 GCGGGGCGCGCGGCCCGGGGGAGGCGACGGTGTCGCGGGAGGAGCATCGGAGCCCGAAGACTCGAAGAAC GCCATGGCCCCCATTGGCCTCAAGGCGGTGGTCGGAGAGAAGATCATGCATGATGTGATCAAGAAGGTGA AGAAGAAGGGCGAGTGGAAGGTGCTGGTGGTGGACCAGTTAAGCATGAGGATGCTGTCCTCCTGCTGCAA GATGACAGACATCATGACCGAGGGCATCACAATTGTGGAGGATATCAACAAGCGCCGAGAGCCACTCCCC AGCCTGGAGGCCGTGTACCTCATCACCCCATCTGAGAAGTCTGTCCACTCTCTGATCAGTGATTTTAAGG ACCCGCCGACTGCTAAATATCGGGCTGCGCATGTGTTCTTCACAGACTCGTGTCCAGATGCCCTATTTAA CGAGCTGGTAAAATCCCGAGCAGCCAAAGTCATCAAGACGCTGACGGAAATCAACATTGCGTTTCTCCCC TATGAGTCCCAGGTGTATTCCCTGGACTCCGCTGACTCTTTCCAAAGCTTCTACAGCCCTCACAAGGCGC AGATGAAGAATCCGATACTGGAACGCCTGGCAGAGCAGATCGCAACCCTGTGTGCCACCCTGAAGGAGTA TCCAGCTGTGCGGTATCGGGGGGAGTACAAGGACAATGCCTTGCTGGCTCAGCTGATCCAGGACAAGCTG GATGCCTATAAAGCCGACGATCCAACAATGGGGGAGGGTCCCGACAAGGCACGGTCCCAGCTCCTGATCC TGGATCGTGGCTTTGACCCCAGCTCCCCTGTGCTCCATGAACTGACATTCCAGGCTATGAGTTATGACCT GCTGCCTATCGAAAATGATGTTTACAAGTATGAGACCAGCGGCATTGGAGAGGCGCGGGTGAAGGAGGTG CTACTGGATGAGGACGATGACCTGTGGATTGCGCTGCGACACAAGCACATCGCAGAGGTGTCCCAGGAAG TCACCCGGTCTCTGAAGGACTTTTCCTCTAGCAAGAGGATGAACACTGGCGAGAAGACCACCATGCGGGA CCTGTCCCAGATGCTGAAGAAAATGCCCCAGTACCAGAAGGAGCTCAGCAAGTATTCGACTCACCTGCAC CTTGCTGAAGACTGCATGAAGCATTACCAAGGCACTGTAGACAAACTCTGCCGCGTGGAGCAGGACCTGG CAATGGGCACAGATGCTGAGGGGGAAAAAATCAAGGACCCCATGAGAGCCATTGTCCCCATCCTGCTGGA TGCGAACGTCAGCACTTACGACAAAATCCGTATCATCCTTCTCTACATCTTCCTGAAGAACGGTATCACT GAGGAGAACCTAAACAAACTCATCCAGCACGCTCAGATACCCCCAGAGGACAGCGAGATCATCACCAACA TGGCTCACCTCGGCGTGCCCATCGTCACGGATTCCACACTACGCCGCCGAAGCAAACCGGAGCGGAAGGA GCGTATCAGTGAGCAGACCTACCAGCTCTCACGATGGACCCCGATCATTAAAGACATTATGGAGGACACT ATCGAAGACAAGCTGGATACAAAGCACTACCCATACATCTCTACCCGCTCGTCCGCGTCCTTCAGCACCA CTGCTGTGAGTGCCCGCTATGGACATTGGCACAAGAATAAGGCCCCCGGGGAGTACCGCAGCGGTCCCCG CCTCATTATTTTCATCCTTGGGGGTGTGAGCCTGAATGAGATGCGCTGTGCTTACGAAGTGACCCAGGCC AACGGCAAGTGGGAAGTGCTGATAGGTTCTACTCACATTCTCACTCCCACCAAATTCCTCATGGACCTGA GACACCCCGACTTCAGGGAGTCCTCTAGGGTATCTTTTGAGGATCAGGCTCCAACAATGGAGTGAGAGCC AAAGAGACAAAGATCCACGCACATTCTCACCCCACAGAAACTGCTGGACACGCTGAAGAAGCTGAATAAA ACAGATGAAGAAATAAGCAGTTAAAAAATAAGCTGCCCCCCAAAACCCCGGCTCCCTTCCCAAAATGCTC TGCAGCTCCCCCGTGCGCCACCTCGGTTACTCTGCTGCCTCCCCAGCCCTGCACGCCCTGGCCACCCCGT TGCCGTGCTGAGTTCTTCTCCTGTGCGATGACACCCCATCTTGTCCTCTGAAAAGCAAGAGAGTAATGTG TTGTTTTTTAAAAATGAGCATCTTCTGTATGTATCCCACAGTAAGTTCACATGCAAGCTCCACACTGCAG AAGCGTCAGAACTCCGGACCGAGTGAATTCTCCCTTATTTATGACCCCGTGACCTGTATATAGCCCTGTC CCGCGTGTGCACATTGCTTGAATATGGAAAGGTAGATGTGTGGGTGTCTCTCCAAGCTTGGTTGGATTCA TTTCTGTCCTTGTTGGTGTTTGTTCCCCGGATAGGACATGCTGAGGGAGTGATGTTCTCGCTAGCCCCTG CTCGCTCCCTGTTCTCAGCGATGAGCAGACACCTCTGGAGGCTGGCGTGGAACGAGCCTCCTCTTTGCAC CTATGGGGGAGGCTTAGGGTGTCCACAGGAAGCCAGTCTGAGTGCCGGCCAGTGTGGTCTCCAGAGCCTG GCACTGCTTTCCCTGATCTGTGTCCATACTGTTGTAACAAGTTAAGCCCTTCAGGCTAAATCAGCCTGCC TAGTGCCCTCGGAGCCTCCAGAGTTAGGTCTGACCAGCCCCCTGCTTGAACACAGTTTGGATAGAGGCCA AGGGTCAGGGGTGGGCTGGAAGCTGTGAGTTTGGCACTCTGGTCAAGGGTGCTTTGCTGTAGGAGCTAGG CCTGAAGAATGGGGGCCCTGCTGCTTAGTCAGAGTCCCCTCAGTTTAAGATACTTCATCAATCTTAAGTT TGTGTAGTGTACAGTCATGTGTCATTGTGGTTGTATGAAAAGGATCATTTTATTCTTTGTATTAGTCATC ACTGTATAAAACATAGCTAGCTATAAAGCAGAAATTCCAGAAGCCGATGCTGGAAGGATGGTCTCCACCC TCAGGACGCAGCAGCCCCCTGAGCATGCTGCTCACCCTGCTGCTGTAGTCGTGAAACAAAGACAGTGGAA GTCACAAAAATGTCCCCAGCCCAGTCCCCGCCCCTCCCTCCCGCAGATTTGTACGTATTACTGTGTCTCG TGCTGTCTTCGCAAACGTGGTGTACGCCTGCCGCAGGTGTCCTGTGCCCTTCTCCCTCCCTGACTCTAGA GTCTCTCTTCTCCTTAGTTCTCAGGCCTCTCCCCTGCTCCTCTCCAGTGAACCTTTTCCCTTAGGACTGA ACCACACTAGCACCGGTTGATTTCTTCTGTAGCGCTTCTCCCATCCCTTCCTCCGGTCAAGCAATGCTCA TGCTTCAGGATCTTGTTTGTCGAACATGTGGGGTTTCCTTTATGTTATTTATATAAATAATTTCTCAAAT GGATATTTAAAAAAAAGCTAGTCTGTCTTGAAACTTGTTAACTTGAAACTCTTGAATCTCAGTGTTTAAA GTATGGAAGCACAACCGTGTACCGCTCTGTACCGTCCTGTACTGCAGCATTTGAGTCTAATAAAGACGTC AGCTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4480978 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4480978 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004017 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source