EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30347

6030446I19Rik (6030446I19Rik)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_017771_01	euxassay_017771_02	euxassay_017771_03	euxassay_017771_04	euxassay_017771_05

euxassay_017771_06	euxassay_017771_07	euxassay_017771_08	euxassay_017771_09	euxassay_017771_10

euxassay_017771_11	euxassay_017771_12	euxassay_017771_13	euxassay_017771_14	euxassay_017771_15

euxassay_017771_16	euxassay_017771_17	euxassay_017771_18	euxassay_017771_19	euxassay_017771_20

euxassay_017771_21	euxassay_017771_22	euxassay_017771_23	euxassay_017771_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T63120
Entity Detected:	6030446I19Rik, (6030446I19Rik)
Sequence:	sense strand is shown >T63120 AACCTGTCTGCCTACCTCACATCACACGGCTATTCTGAGGATCCGAGAGACAAACAGGCAGAAAACTCCA GTCTGGTGGGAAGAACTGGCTGCCCTTGGGTGTTGGAAAGTGTCTTGCCCACCTAGAGTTGAAAAGGCTT CTTGGGACCCTAGCAAGAAGGCCAGAGAGGTGGAAGAACGGAGATGCTATTATCTTCTGTCCCTGACCCC TGCAAACTCACCCCTTTGCCTGCAGTTCTGTTGCCAAGGAAGGCTCTGTTGTGTGCCAAACAACAGTGTC CAGCAGATGGACATCAGGCAGCTTCTGCATTGGCGCTCAGGCTGCAGAAGCCGATTACATCCTGTGGACA CTCAGTGCAAGTGACTTCTGACAACTCCTCTCCCAGGGCATTGGCCTGAAGGTTTAGCCTCCCTTGAACT TTCCAGAGGCCTCTGACTTGGAGATGTGTCCTTTGTCATCTTTGTTTATCAGGCAGTGCCTTCTCACAGG AGTTCCCATGTGCAAACGCCAGGTGACTTTTACACAAGGAAGCTCGGTGTCTCCAGGTTTCAGCTGCCTC CCAAGGCACAGACTTGGGGGAACCAGGAGATCAGTAGAAGCTTTGTTAAGAGACCCTGGATGCTAAGCGA GAAGGCCTACTTGGTTCTTACTCTGTAGTGGTGAGGCCACTTTGCCCCGAGCTATCTCCATGCCCCTTGG GAGAGGGGAGAGGAAACAGGCTCTTGGGTGAACTGTTTCAGTTGCCAGCTCCTAAGATGATTCTTGTGGA ATGCCAACGGAACGAAACATCCCAGTTCCACGTCAGTGTCACCAATGTGGCCTACACAAGTACTCTCACT TGGGGGAGATGGTGTTCTTGCTGAGACAAGGGGGAGGAGTGGAGCTTGTTCCTTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 76467. Forward Primer - name:076467_F_cDNA_BC043114, sequence:AACCTGTCTGCCTACCTCACAT; Reverse Primer - name:076467_N_SP6_cDNA_BC043114, sequence:AAAGGAACAAGCTCCACTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_017771 same experiment
	EMAGE:30390 same embryo
	EMAGE:30373 same embryo
	EMAGE:30388 same embryo
	EMAGE:30366 same embryo
	EMAGE:30363 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	BrainStars same gene
	ViBrism same gene
Data Source